Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2937 and GFF2938 are separated by 4 nucleotides GFF2938 and GFF2939 are separated by 7 nucleotides GFF2939 and GFF2940 are separated by 212 nucleotides
GFF2937 - Uncharacterized ABC transporter, periplasmic component YrbD, at 19,076 to 19,627
GFF2937
GFF2938 - Uncharacterized ABC transporter, permease component YrbE, at 19,632 to 20,414
GFF2938
GFF2939 - Uncharacterized ABC transporter, ATP-binding protein YrbF, at 20,422 to 21,234
GFF2939
GFF2940 - Inner membrane protein YrbG, predicted calcium/sodium:proton antiporter, at 21,447 to 22,424
GFF2940
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-1
0
1 at 19.529 kb on - strand, within GFF2937 at 19.751 kb on - strand, within GFF2938 at 19.827 kb on - strand, within GFF2938 at 19.934 kb on + strand, within GFF2938 at 19.934 kb on + strand, within GFF2938 at 19.944 kb on + strand, within GFF2938 at 20.174 kb on - strand, within GFF2938 at 20.243 kb on + strand, within GFF2938 at 20.423 kb on + strand at 20.423 kb on + strand at 20.424 kb on - strand at 20.605 kb on - strand, within GFF2939 at 20.700 kb on + strand, within GFF2939 at 20.873 kb on - strand, within GFF2939 at 20.913 kb on - strand, within GFF2939 at 20.976 kb on + strand, within GFF2939 at 20.976 kb on + strand, within GFF2939 at 20.977 kb on - strand, within GFF2939 at 21.037 kb on + strand, within GFF2939 at 21.038 kb on - strand, within GFF2939 at 21.097 kb on - strand, within GFF2939 at 21.156 kb on + strand at 21.250 kb on - strand at 21.250 kb on - strand at 21.300 kb on + strand at 21.301 kb on - strand at 21.304 kb on + strand at 21.304 kb on + strand at 21.311 kb on + strand at 21.401 kb on - strand at 21.401 kb on - strand at 21.424 kb on + strand at 21.426 kb on + strand at 21.539 kb on + strand at 21.539 kb on + strand at 21.674 kb on + strand, within GFF2940 at 21.674 kb on + strand, within GFF2940 at 21.674 kb on + strand, within GFF2940 at 21.675 kb on - strand, within GFF2940 at 21.686 kb on + strand, within GFF2940 at 21.700 kb on + strand, within GFF2940 at 21.748 kb on + strand, within GFF2940 at 21.748 kb on + strand, within GFF2940 at 21.749 kb on - strand, within GFF2940 at 21.772 kb on + strand, within GFF2940 at 21.772 kb on + strand, within GFF2940 at 21.833 kb on + strand, within GFF2940 at 21.834 kb on - strand, within GFF2940 at 21.859 kb on + strand, within GFF2940 at 21.860 kb on - strand, within GFF2940 at 21.868 kb on + strand, within GFF2940 at 22.053 kb on + strand, within GFF2940 at 22.053 kb on + strand, within GFF2940
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 19,529 - GFF2937 0.82 -0.9 19,751 - GFF2938 0.15 -0.1 19,827 - GFF2938 0.25 -0.6 19,934 + GFF2938 0.39 -0.7 19,934 + GFF2938 0.39 -1.0 19,944 + GFF2938 0.40 -0.3 20,174 - GFF2938 0.69 -1.1 20,243 + GFF2938 0.78 +0.8 20,423 + -0.5 20,423 + -0.4 20,424 - -0.4 20,605 - GFF2939 0.23 +0.1 20,700 + GFF2939 0.34 +0.0 20,873 - GFF2939 0.55 -0.3 20,913 - GFF2939 0.60 +0.1 20,976 + GFF2939 0.68 -0.7 20,976 + GFF2939 0.68 -0.3 20,977 - GFF2939 0.68 -1.0 21,037 + GFF2939 0.76 -0.0 21,038 - GFF2939 0.76 +0.1 21,097 - GFF2939 0.83 -0.2 21,156 + -0.7 21,250 - -0.7 21,250 - +0.1 21,300 + -0.5 21,301 - -0.2 21,304 + +0.1 21,304 + -0.3 21,311 + -0.2 21,401 - +0.8 21,401 - +0.8 21,424 + -0.4 21,426 + +0.4 21,539 + -1.2 21,539 + -0.3 21,674 + GFF2940 0.23 -0.7 21,674 + GFF2940 0.23 +0.2 21,674 + GFF2940 0.23 +0.0 21,675 - GFF2940 0.23 +1.1 21,686 + GFF2940 0.24 -0.2 21,700 + GFF2940 0.26 +0.4 21,748 + GFF2940 0.31 +0.6 21,748 + GFF2940 0.31 -0.0 21,749 - GFF2940 0.31 +0.3 21,772 + GFF2940 0.33 +0.2 21,772 + GFF2940 0.33 -1.2 21,833 + GFF2940 0.39 -0.4 21,834 - GFF2940 0.40 +0.8 21,859 + GFF2940 0.42 -0.3 21,860 - GFF2940 0.42 +0.1 21,868 + GFF2940 0.43 -0.8 22,053 + GFF2940 0.62 +0.1 22,053 + GFF2940 0.62 -0.0
Or see this region's nucleotide sequence