Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2729 and GFF2730 are separated by 19 nucleotides GFF2730 and GFF2731 are separated by 81 nucleotides GFF2731 and GFF2732 are separated by 337 nucleotides GFF2732 and GFF2733 are separated by 25 nucleotides
GFF2729 - Protein YciE, at 252,025 to 252,531
GFF2729
GFF2730 - Manganese catalase (EC 1.11.1.6), at 252,551 to 253,429
GFF2730
GFF2731 - Outer membrane protein W precursor, at 253,511 to 254,149
GFF2731
GFF2732 - Ferredoxin, at 254,487 to 254,891
GFF2732
GFF2733 - Membrane protein YciC, linked to IspA, at 254,917 to 255,660
GFF2733
Position (kb) 253
254
255 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 252.578 kb on + strand at 252.580 kb on + strand at 252.602 kb on - strand at 252.871 kb on + strand, within GFF2730 at 252.947 kb on + strand, within GFF2730 at 252.950 kb on - strand, within GFF2730 at 252.955 kb on + strand, within GFF2730 at 253.178 kb on + strand, within GFF2730 at 253.193 kb on + strand, within GFF2730 at 253.193 kb on + strand, within GFF2730 at 253.441 kb on + strand at 253.770 kb on + strand, within GFF2731 at 253.825 kb on - strand, within GFF2731 at 253.954 kb on - strand, within GFF2731 at 254.004 kb on - strand, within GFF2731 at 254.035 kb on + strand, within GFF2731 at 254.066 kb on - strand, within GFF2731 at 254.320 kb on + strand at 254.451 kb on + strand at 254.461 kb on + strand at 254.533 kb on - strand, within GFF2732 at 254.533 kb on - strand, within GFF2732 at 254.620 kb on + strand, within GFF2732 at 254.620 kb on + strand, within GFF2732 at 254.628 kb on - strand, within GFF2732 at 254.628 kb on - strand, within GFF2732 at 254.822 kb on + strand, within GFF2732 at 254.835 kb on - strand, within GFF2732 at 254.890 kb on - strand at 255.012 kb on + strand, within GFF2733
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 252,578 + -2.9 252,580 + -0.6 252,602 - -0.0 252,871 + GFF2730 0.36 +1.1 252,947 + GFF2730 0.45 +0.7 252,950 - GFF2730 0.45 -0.1 252,955 + GFF2730 0.46 +0.7 253,178 + GFF2730 0.71 +0.0 253,193 + GFF2730 0.73 +1.2 253,193 + GFF2730 0.73 -0.8 253,441 + -0.0 253,770 + GFF2731 0.41 +1.4 253,825 - GFF2731 0.49 +0.7 253,954 - GFF2731 0.69 +0.4 254,004 - GFF2731 0.77 +1.0 254,035 + GFF2731 0.82 -1.0 254,066 - GFF2731 0.87 +1.3 254,320 + -0.0 254,451 + +0.3 254,461 + -0.5 254,533 - GFF2732 0.11 -0.5 254,533 - GFF2732 0.11 +1.0 254,620 + GFF2732 0.33 +2.3 254,620 + GFF2732 0.33 -0.5 254,628 - GFF2732 0.35 -0.5 254,628 - GFF2732 0.35 -0.5 254,822 + GFF2732 0.83 +1.3 254,835 - GFF2732 0.86 +0.0 254,890 - -3.5 255,012 + GFF2733 0.13 -0.7
Or see this region's nucleotide sequence