Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2634 and GFF2635 are separated by 265 nucleotides GFF2635 and GFF2636 are separated by 7 nucleotides GFF2636 and GFF2637 overlap by 1 nucleotides GFF2637 and GFF2638 are separated by 239 nucleotides
GFF2634 - Gfa-like protein, at 161,657 to 161,983
GFF2634
GFF2635 - Putative uncharacterized protein ydbL, may be related to amine metabolism, at 162,249 to 162,572
GFF2635
GFF2636 - Uncharacterized protein ynbE; probable lipoprotein STY1424, at 162,580 to 162,771
GFF2636
GFF2637 - Putative uncharacterized protein ydbH, at 162,771 to 165,407
GFF2637
GFF2638 - D-lactate dehydrogenase (EC 1.1.1.28), at 165,647 to 166,636
GFF2638
Position (kb) 162
163
164
165
166 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 161.864 kb on - strand, within GFF2634 at 161.864 kb on - strand, within GFF2634 at 161.866 kb on - strand, within GFF2634 at 162.029 kb on - strand at 162.114 kb on + strand at 162.115 kb on - strand at 162.126 kb on + strand at 162.370 kb on - strand, within GFF2635 at 162.553 kb on - strand at 162.553 kb on - strand at 162.636 kb on + strand, within GFF2636 at 162.705 kb on + strand, within GFF2636 at 162.729 kb on + strand, within GFF2636 at 162.777 kb on - strand at 162.824 kb on + strand at 162.930 kb on - strand at 162.987 kb on - strand at 163.027 kb on + strand at 163.027 kb on + strand at 163.345 kb on - strand, within GFF2637 at 163.425 kb on + strand, within GFF2637 at 163.830 kb on - strand, within GFF2637 at 163.896 kb on + strand, within GFF2637 at 163.896 kb on + strand, within GFF2637 at 163.931 kb on + strand, within GFF2637 at 163.931 kb on + strand, within GFF2637 at 164.027 kb on + strand, within GFF2637 at 164.079 kb on - strand, within GFF2637 at 164.099 kb on - strand, within GFF2637 at 164.199 kb on - strand, within GFF2637 at 164.237 kb on + strand, within GFF2637 at 164.386 kb on - strand, within GFF2637 at 164.419 kb on - strand, within GFF2637 at 164.436 kb on - strand, within GFF2637 at 164.508 kb on - strand, within GFF2637 at 164.631 kb on - strand, within GFF2637 at 164.905 kb on - strand, within GFF2637 at 164.905 kb on - strand, within GFF2637 at 164.943 kb on + strand, within GFF2637 at 164.944 kb on - strand, within GFF2637 at 165.027 kb on - strand, within GFF2637 at 165.035 kb on + strand, within GFF2637 at 165.096 kb on - strand, within GFF2637 at 165.140 kb on + strand, within GFF2637 at 165.141 kb on - strand, within GFF2637 at 165.188 kb on + strand at 165.342 kb on + strand at 165.369 kb on + strand at 165.393 kb on - strand at 165.485 kb on + strand at 165.665 kb on - strand at 165.693 kb on + strand at 166.337 kb on - strand, within GFF2638 at 166.339 kb on + strand, within GFF2638 at 166.339 kb on + strand, within GFF2638 at 166.340 kb on - strand, within GFF2638
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 161,864 - GFF2634 0.63 +0.2 161,864 - GFF2634 0.63 +0.3 161,866 - GFF2634 0.64 -0.4 162,029 - +0.6 162,114 + -0.1 162,115 - -0.3 162,126 + +0.2 162,370 - GFF2635 0.37 +0.1 162,553 - -0.2 162,553 - -0.7 162,636 + GFF2636 0.29 -0.7 162,705 + GFF2636 0.65 +0.4 162,729 + GFF2636 0.78 +0.8 162,777 - -0.7 162,824 + -0.8 162,930 - +0.1 162,987 - -0.1 163,027 + -0.7 163,027 + -0.9 163,345 - GFF2637 0.22 -0.1 163,425 + GFF2637 0.25 -0.7 163,830 - GFF2637 0.40 -1.8 163,896 + GFF2637 0.43 -0.1 163,896 + GFF2637 0.43 -0.7 163,931 + GFF2637 0.44 -2.1 163,931 + GFF2637 0.44 +0.1 164,027 + GFF2637 0.48 -0.4 164,079 - GFF2637 0.50 -0.1 164,099 - GFF2637 0.50 -0.3 164,199 - GFF2637 0.54 -0.8 164,237 + GFF2637 0.56 -2.6 164,386 - GFF2637 0.61 +0.2 164,419 - GFF2637 0.62 -0.1 164,436 - GFF2637 0.63 -0.3 164,508 - GFF2637 0.66 +0.2 164,631 - GFF2637 0.71 -0.4 164,905 - GFF2637 0.81 -0.6 164,905 - GFF2637 0.81 -0.5 164,943 + GFF2637 0.82 +0.6 164,944 - GFF2637 0.82 -0.2 165,027 - GFF2637 0.86 -1.2 165,035 + GFF2637 0.86 -1.8 165,096 - GFF2637 0.88 -0.9 165,140 + GFF2637 0.90 +0.3 165,141 - GFF2637 0.90 -0.2 165,188 + -0.2 165,342 + -0.8 165,369 + -0.1 165,393 - +0.1 165,485 + -1.3 165,665 - +0.5 165,693 + -0.8 166,337 - GFF2638 0.70 -3.3 166,339 + GFF2638 0.70 +0.6 166,339 + GFF2638 0.70 -0.7 166,340 - GFF2638 0.70 -0.5
Or see this region's nucleotide sequence