Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2580 and GFF2581 are separated by 211 nucleotides GFF2581 and GFF2582 overlap by 1 nucleotides GFF2582 and GFF2583 are separated by 4 nucleotides GFF2583 and GFF2584 are separated by 223 nucleotides
GFF2580 - Putative oxidoreductase YncB, at 102,714 to 103,751
GFF2580
GFF2581 - Putative acetyltransferase, at 103,963 to 104,478
GFF2581
GFF2582 - Putative inner membrane protein, at 104,478 to 104,927
GFF2582
GFF2583 - FIG00638150: hypothetical protein, at 104,932 to 105,165
GFF2583
GFF2584 - Putative virulence effector protein, at 105,389 to 106,708
GFF2584
Position (kb) 103
104
105 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 102.994 kb on + strand, within GFF2580 at 103.035 kb on - strand, within GFF2580 at 103.087 kb on + strand, within GFF2580 at 103.087 kb on + strand, within GFF2580 at 103.087 kb on + strand, within GFF2580 at 103.092 kb on - strand, within GFF2580 at 103.340 kb on - strand, within GFF2580 at 103.561 kb on + strand, within GFF2580 at 103.592 kb on + strand, within GFF2580 at 103.593 kb on - strand, within GFF2580 at 103.593 kb on - strand, within GFF2580 at 103.593 kb on - strand, within GFF2580 at 103.593 kb on - strand, within GFF2580 at 103.593 kb on - strand, within GFF2580 at 103.594 kb on + strand, within GFF2580 at 103.594 kb on + strand, within GFF2580 at 103.595 kb on - strand, within GFF2580 at 103.627 kb on - strand, within GFF2580 at 103.785 kb on + strand at 103.785 kb on + strand at 103.848 kb on + strand at 103.925 kb on - strand at 103.942 kb on - strand at 104.016 kb on + strand, within GFF2581 at 104.030 kb on + strand, within GFF2581 at 104.030 kb on + strand, within GFF2581 at 104.030 kb on + strand, within GFF2581 at 104.030 kb on + strand, within GFF2581 at 104.031 kb on - strand, within GFF2581 at 104.035 kb on - strand, within GFF2581 at 104.105 kb on + strand, within GFF2581 at 104.197 kb on + strand, within GFF2581 at 104.351 kb on + strand, within GFF2581 at 104.371 kb on - strand, within GFF2581 at 104.544 kb on - strand, within GFF2582 at 104.546 kb on + strand, within GFF2582 at 104.556 kb on - strand, within GFF2582 at 104.588 kb on + strand, within GFF2582 at 104.620 kb on + strand, within GFF2582 at 104.642 kb on + strand, within GFF2582 at 104.643 kb on - strand, within GFF2582 at 104.926 kb on - strand at 105.041 kb on + strand, within GFF2583 at 105.166 kb on - strand at 105.210 kb on - strand at 105.210 kb on - strand at 105.211 kb on + strand at 105.211 kb on + strand at 105.266 kb on - strand at 105.292 kb on + strand at 105.293 kb on - strand at 105.428 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 102,994 + GFF2580 0.27 -0.2 103,035 - GFF2580 0.31 -0.5 103,087 + GFF2580 0.36 -0.8 103,087 + GFF2580 0.36 +0.2 103,087 + GFF2580 0.36 -0.5 103,092 - GFF2580 0.36 +0.1 103,340 - GFF2580 0.60 -0.3 103,561 + GFF2580 0.82 +0.5 103,592 + GFF2580 0.85 -0.2 103,593 - GFF2580 0.85 -0.0 103,593 - GFF2580 0.85 -0.2 103,593 - GFF2580 0.85 +0.1 103,593 - GFF2580 0.85 +0.6 103,593 - GFF2580 0.85 +0.3 103,594 + GFF2580 0.85 -1.6 103,594 + GFF2580 0.85 -1.4 103,595 - GFF2580 0.85 -1.2 103,627 - GFF2580 0.88 +0.2 103,785 + -0.6 103,785 + +0.2 103,848 + -0.7 103,925 - -0.3 103,942 - -0.4 104,016 + GFF2581 0.10 +0.2 104,030 + GFF2581 0.13 -0.9 104,030 + GFF2581 0.13 -0.5 104,030 + GFF2581 0.13 -1.9 104,030 + GFF2581 0.13 -1.1 104,031 - GFF2581 0.13 +0.1 104,035 - GFF2581 0.14 -0.9 104,105 + GFF2581 0.28 +0.6 104,197 + GFF2581 0.45 +0.0 104,351 + GFF2581 0.75 -0.6 104,371 - GFF2581 0.79 -1.3 104,544 - GFF2582 0.15 -0.8 104,546 + GFF2582 0.15 +0.2 104,556 - GFF2582 0.17 -1.2 104,588 + GFF2582 0.24 +0.1 104,620 + GFF2582 0.32 -0.5 104,642 + GFF2582 0.36 +0.2 104,643 - GFF2582 0.37 -0.8 104,926 - +0.5 105,041 + GFF2583 0.47 -0.3 105,166 - -1.3 105,210 - -0.5 105,210 - -0.4 105,211 + -2.8 105,211 + +0.4 105,266 - -0.3 105,292 + -0.5 105,293 - -0.8 105,428 + -2.8
Or see this region's nucleotide sequence