Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2567 and GFF2568 are separated by 91 nucleotides GFF2568 and GFF2569 overlap by 4 nucleotides
GFF2567 - Nitrate/nitrite transporter, at 84,975 to 86,213
GFF2567
GFF2568 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 86,305 to 90,045
GFF2568
GFF2569 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 90,042 to 91,586
GFF2569
Position (kb)
86
87
88
89
90
91 Strain fitness (log2 ratio)
-2
-1
0
1 at 85.682 kb on - strand, within GFF2567 at 85.822 kb on + strand, within GFF2567 at 85.823 kb on - strand, within GFF2567 at 85.869 kb on + strand, within GFF2567 at 86.219 kb on + strand at 86.220 kb on - strand at 86.220 kb on - strand at 86.220 kb on - strand at 86.220 kb on - strand at 86.310 kb on + strand at 86.310 kb on + strand at 86.600 kb on - strand at 86.614 kb on + strand at 86.615 kb on - strand at 86.661 kb on - strand at 86.779 kb on - strand, within GFF2568 at 86.846 kb on - strand, within GFF2568 at 86.920 kb on + strand, within GFF2568 at 87.038 kb on - strand, within GFF2568 at 87.039 kb on + strand, within GFF2568 at 87.040 kb on - strand, within GFF2568 at 87.172 kb on - strand, within GFF2568 at 87.281 kb on - strand, within GFF2568 at 87.355 kb on + strand, within GFF2568 at 87.355 kb on + strand, within GFF2568 at 87.356 kb on - strand, within GFF2568 at 87.369 kb on + strand, within GFF2568 at 87.745 kb on + strand, within GFF2568 at 87.786 kb on + strand, within GFF2568 at 87.787 kb on - strand, within GFF2568 at 87.790 kb on + strand, within GFF2568 at 87.864 kb on + strand, within GFF2568 at 87.895 kb on - strand, within GFF2568 at 87.946 kb on + strand, within GFF2568 at 87.964 kb on + strand, within GFF2568 at 88.258 kb on - strand, within GFF2568 at 88.351 kb on - strand, within GFF2568 at 88.480 kb on + strand, within GFF2568 at 88.551 kb on + strand, within GFF2568 at 88.551 kb on + strand, within GFF2568 at 88.636 kb on - strand, within GFF2568 at 88.636 kb on - strand, within GFF2568 at 88.679 kb on - strand, within GFF2568 at 88.698 kb on + strand, within GFF2568 at 88.698 kb on + strand, within GFF2568 at 88.780 kb on + strand, within GFF2568 at 88.850 kb on + strand, within GFF2568 at 88.899 kb on + strand, within GFF2568 at 88.899 kb on + strand, within GFF2568 at 88.933 kb on - strand, within GFF2568 at 88.933 kb on - strand, within GFF2568 at 88.957 kb on - strand, within GFF2568 at 88.991 kb on - strand, within GFF2568 at 89.026 kb on - strand, within GFF2568 at 89.486 kb on - strand, within GFF2568 at 89.809 kb on - strand at 89.857 kb on - strand at 89.892 kb on + strand at 90.049 kb on + strand at 90.089 kb on + strand at 90.154 kb on - strand at 90.252 kb on - strand, within GFF2569 at 90.296 kb on + strand, within GFF2569 at 90.297 kb on - strand, within GFF2569 at 90.530 kb on - strand, within GFF2569 at 90.544 kb on - strand, within GFF2569 at 90.573 kb on + strand, within GFF2569 at 90.643 kb on - strand, within GFF2569 at 90.684 kb on - strand, within GFF2569 at 90.916 kb on - strand, within GFF2569 at 90.977 kb on + strand, within GFF2569
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 85,682 - GFF2567 0.57 +0.2 85,822 + GFF2567 0.68 -0.4 85,823 - GFF2567 0.68 -0.8 85,869 + GFF2567 0.72 +0.2 86,219 + +0.1 86,220 - -0.4 86,220 - -0.4 86,220 - +0.3 86,220 - -0.5 86,310 + +0.1 86,310 + -0.3 86,600 - +0.9 86,614 + +0.5 86,615 - -0.1 86,661 - +0.2 86,779 - GFF2568 0.13 -0.5 86,846 - GFF2568 0.14 -0.5 86,920 + GFF2568 0.16 -0.1 87,038 - GFF2568 0.20 -0.4 87,039 + GFF2568 0.20 -0.7 87,040 - GFF2568 0.20 +1.1 87,172 - GFF2568 0.23 -0.1 87,281 - GFF2568 0.26 -0.6 87,355 + GFF2568 0.28 -0.3 87,355 + GFF2568 0.28 +0.0 87,356 - GFF2568 0.28 -0.8 87,369 + GFF2568 0.28 +0.2 87,745 + GFF2568 0.38 +0.7 87,786 + GFF2568 0.40 +0.1 87,787 - GFF2568 0.40 -0.2 87,790 + GFF2568 0.40 -1.8 87,864 + GFF2568 0.42 -0.3 87,895 - GFF2568 0.43 -0.7 87,946 + GFF2568 0.44 -0.3 87,964 + GFF2568 0.44 -0.3 88,258 - GFF2568 0.52 -0.5 88,351 - GFF2568 0.55 -1.4 88,480 + GFF2568 0.58 +0.2 88,551 + GFF2568 0.60 -2.3 88,551 + GFF2568 0.60 -0.4 88,636 - GFF2568 0.62 -0.8 88,636 - GFF2568 0.62 -0.3 88,679 - GFF2568 0.63 +0.2 88,698 + GFF2568 0.64 -0.5 88,698 + GFF2568 0.64 -0.2 88,780 + GFF2568 0.66 -1.2 88,850 + GFF2568 0.68 -1.3 88,899 + GFF2568 0.69 -0.1 88,899 + GFF2568 0.69 -0.4 88,933 - GFF2568 0.70 -1.2 88,933 - GFF2568 0.70 +0.7 88,957 - GFF2568 0.71 -0.0 88,991 - GFF2568 0.72 -0.0 89,026 - GFF2568 0.73 -1.6 89,486 - GFF2568 0.85 +1.4 89,809 - +1.3 89,857 - -0.8 89,892 + -0.3 90,049 + -0.8 90,089 + +0.4 90,154 - -0.1 90,252 - GFF2569 0.14 -0.5 90,296 + GFF2569 0.16 +0.1 90,297 - GFF2569 0.17 +0.2 90,530 - GFF2569 0.32 -0.1 90,544 - GFF2569 0.32 +0.1 90,573 + GFF2569 0.34 +0.2 90,643 - GFF2569 0.39 -0.4 90,684 - GFF2569 0.42 -1.8 90,916 - GFF2569 0.57 -1.3 90,977 + GFF2569 0.61 -1.3
Or see this region's nucleotide sequence