Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2503 and GFF2504 are separated by 227 nucleotides GFF2504 and GFF2505 are separated by 118 nucleotides GFF2505 and GFF2506 are separated by 28 nucleotides GFF2506 and GFF2507 are separated by 19 nucleotides
GFF2503 - Putative transport protein, at 20,031 to 21,218
GFF2503
GFF2504 - Permease of the drug/metabolite transporter (DMT) superfamily, at 21,446 to 22,348
GFF2504
GFF2505 - Multiple antibiotic resistance protein MarB, at 22,467 to 22,682
GFF2505
GFF2506 - Multiple antibiotic resistance protein MarA, at 22,711 to 23,094
GFF2506
GFF2507 - Multiple antibiotic resistance protein MarR, at 23,114 to 23,491
GFF2507
Position (kb) 21
22
23 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 20.534 kb on + strand, within GFF2503 at 20.595 kb on + strand, within GFF2503 at 20.596 kb on - strand, within GFF2503 at 20.733 kb on + strand, within GFF2503 at 20.807 kb on - strand, within GFF2503 at 20.807 kb on - strand, within GFF2503 at 21.121 kb on + strand at 21.121 kb on + strand at 21.122 kb on - strand at 21.131 kb on + strand at 21.165 kb on + strand at 21.166 kb on - strand at 21.290 kb on - strand at 21.297 kb on - strand at 21.298 kb on + strand at 21.298 kb on + strand at 21.304 kb on - strand at 21.310 kb on - strand at 21.314 kb on + strand at 21.638 kb on + strand, within GFF2504 at 21.638 kb on + strand, within GFF2504 at 21.823 kb on - strand, within GFF2504 at 21.823 kb on - strand, within GFF2504 at 21.865 kb on + strand, within GFF2504 at 21.865 kb on + strand, within GFF2504 at 21.865 kb on + strand, within GFF2504 at 21.865 kb on + strand, within GFF2504 at 21.866 kb on - strand, within GFF2504 at 21.866 kb on - strand, within GFF2504 at 21.866 kb on - strand, within GFF2504 at 21.868 kb on - strand, within GFF2504 at 21.883 kb on + strand, within GFF2504 at 22.067 kb on - strand, within GFF2504 at 22.070 kb on - strand, within GFF2504 at 22.079 kb on - strand, within GFF2504 at 22.089 kb on + strand, within GFF2504 at 22.296 kb on - strand at 22.297 kb on + strand at 22.327 kb on + strand at 22.355 kb on - strand at 22.362 kb on + strand at 22.560 kb on + strand, within GFF2505 at 22.712 kb on + strand at 22.713 kb on - strand at 22.715 kb on + strand at 22.741 kb on - strand at 22.741 kb on - strand at 22.762 kb on - strand, within GFF2506 at 22.844 kb on + strand, within GFF2506 at 22.845 kb on - strand, within GFF2506 at 22.925 kb on + strand, within GFF2506 at 23.064 kb on + strand at 23.064 kb on + strand at 23.065 kb on - strand at 23.065 kb on - strand at 23.065 kb on - strand at 23.071 kb on - strand at 23.116 kb on - strand at 23.116 kb on - strand at 23.139 kb on + strand at 23.180 kb on + strand, within GFF2507
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 20,534 + GFF2503 0.42 +0.7 20,595 + GFF2503 0.47 +0.3 20,596 - GFF2503 0.48 +0.9 20,733 + GFF2503 0.59 -1.0 20,807 - GFF2503 0.65 +0.6 20,807 - GFF2503 0.65 -1.1 21,121 + +1.6 21,121 + +0.2 21,122 - +1.5 21,131 + +0.3 21,165 + +2.1 21,166 - +0.1 21,290 - -0.3 21,297 - -0.8 21,298 + +1.1 21,298 + +0.0 21,304 - -0.1 21,310 - +0.2 21,314 + -0.3 21,638 + GFF2504 0.21 -0.2 21,638 + GFF2504 0.21 +0.7 21,823 - GFF2504 0.42 +2.8 21,823 - GFF2504 0.42 +0.4 21,865 + GFF2504 0.46 +0.3 21,865 + GFF2504 0.46 -0.2 21,865 + GFF2504 0.46 +0.3 21,865 + GFF2504 0.46 +1.5 21,866 - GFF2504 0.47 +0.8 21,866 - GFF2504 0.47 +0.3 21,866 - GFF2504 0.47 +0.2 21,868 - GFF2504 0.47 +0.3 21,883 + GFF2504 0.48 -0.1 22,067 - GFF2504 0.69 +0.2 22,070 - GFF2504 0.69 +0.7 22,079 - GFF2504 0.70 -1.3 22,089 + GFF2504 0.71 -0.1 22,296 - +1.7 22,297 + +0.2 22,327 + -0.3 22,355 - -0.8 22,362 + +0.2 22,560 + GFF2505 0.43 +0.2 22,712 + +1.6 22,713 - +0.7 22,715 + +0.4 22,741 - +1.4 22,741 - -0.8 22,762 - GFF2506 0.13 -0.3 22,844 + GFF2506 0.35 -2.2 22,845 - GFF2506 0.35 +1.2 22,925 + GFF2506 0.56 +0.1 23,064 + +0.7 23,064 + +0.6 23,065 - +0.5 23,065 - -0.1 23,065 - -0.4 23,071 - +0.4 23,116 - +0.7 23,116 - -0.0 23,139 + +0.7 23,180 + GFF2507 0.17 -0.1
Or see this region's nucleotide sequence