Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2349 and GFF2350 are separated by 15 nucleotides GFF2350 and GFF2351 are separated by 46 nucleotides GFF2351 and GFF2352 are separated by 373 nucleotides
GFF2349 - Unsaturated fatty acid biosythesis repressor FabR, TetR family, at 144,506 to 145,138
GFF2349
GFF2350 - Putative inner membrane protein, at 145,154 to 145,513
GFF2350
GFF2351 - tRNA (uracil(54)-C5)-methyltransferase (EC 2.1.1.35), at 145,560 to 146,660
GFF2351
GFF2352 - Outer membrane vitamin B12 receptor BtuB, at 147,034 to 148,878
GFF2352
Position (kb)
145
146
147 Strain fitness (log2 ratio)
-2
-1
0
1 at 144.800 kb on - strand, within GFF2349 at 144.917 kb on + strand, within GFF2349 at 144.978 kb on + strand, within GFF2349 at 144.978 kb on + strand, within GFF2349 at 144.979 kb on - strand, within GFF2349 at 144.982 kb on + strand, within GFF2349 at 144.982 kb on + strand, within GFF2349 at 144.982 kb on + strand, within GFF2349 at 145.089 kb on - strand at 145.091 kb on + strand at 145.119 kb on + strand at 145.152 kb on + strand at 145.181 kb on - strand at 145.217 kb on - strand, within GFF2350 at 145.274 kb on - strand, within GFF2350 at 145.293 kb on + strand, within GFF2350 at 145.294 kb on - strand, within GFF2350 at 145.295 kb on + strand, within GFF2350 at 145.295 kb on + strand, within GFF2350 at 145.295 kb on + strand, within GFF2350 at 145.296 kb on - strand, within GFF2350 at 145.325 kb on - strand, within GFF2350 at 145.327 kb on + strand, within GFF2350 at 145.328 kb on - strand, within GFF2350 at 145.328 kb on - strand, within GFF2350 at 145.328 kb on - strand, within GFF2350 at 145.380 kb on + strand, within GFF2350 at 145.385 kb on + strand, within GFF2350 at 145.385 kb on + strand, within GFF2350 at 145.385 kb on + strand, within GFF2350 at 145.386 kb on - strand, within GFF2350 at 145.386 kb on - strand, within GFF2350 at 145.387 kb on + strand, within GFF2350 at 145.387 kb on + strand, within GFF2350 at 145.388 kb on - strand, within GFF2350 at 145.477 kb on + strand, within GFF2350 at 145.535 kb on + strand at 145.535 kb on + strand at 145.554 kb on + strand at 145.555 kb on - strand at 145.593 kb on - strand at 145.601 kb on + strand at 145.601 kb on + strand at 145.603 kb on + strand at 145.706 kb on + strand, within GFF2351 at 145.912 kb on + strand, within GFF2351 at 146.008 kb on + strand, within GFF2351 at 146.009 kb on - strand, within GFF2351 at 146.072 kb on + strand, within GFF2351 at 146.120 kb on - strand, within GFF2351 at 146.160 kb on - strand, within GFF2351 at 146.160 kb on - strand, within GFF2351 at 146.285 kb on + strand, within GFF2351 at 146.290 kb on + strand, within GFF2351 at 146.332 kb on + strand, within GFF2351 at 146.352 kb on - strand, within GFF2351 at 146.405 kb on + strand, within GFF2351 at 146.474 kb on + strand, within GFF2351 at 146.474 kb on + strand, within GFF2351 at 146.474 kb on + strand, within GFF2351 at 146.475 kb on - strand, within GFF2351 at 146.482 kb on + strand, within GFF2351 at 146.526 kb on - strand, within GFF2351 at 146.526 kb on - strand, within GFF2351 at 146.554 kb on - strand at 146.588 kb on + strand at 146.589 kb on - strand at 146.629 kb on + strand at 146.629 kb on + strand at 146.630 kb on - strand at 146.630 kb on - strand at 146.630 kb on - strand at 146.630 kb on - strand at 146.630 kb on - strand at 146.723 kb on - strand at 146.805 kb on + strand at 146.805 kb on + strand at 146.806 kb on - strand at 146.806 kb on - strand at 146.871 kb on - strand at 146.871 kb on - strand at 146.985 kb on - strand at 147.015 kb on - strand at 147.015 kb on - strand at 147.016 kb on + strand at 147.016 kb on + strand at 147.320 kb on + strand, within GFF2352 at 147.320 kb on + strand, within GFF2352 at 147.321 kb on - strand, within GFF2352 at 147.321 kb on - strand, within GFF2352 at 147.418 kb on + strand, within GFF2352 at 147.418 kb on + strand, within GFF2352 at 147.418 kb on + strand, within GFF2352 at 147.477 kb on + strand, within GFF2352 at 147.483 kb on + strand, within GFF2352 at 147.534 kb on + strand, within GFF2352 at 147.534 kb on + strand, within GFF2352
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 144,800 - GFF2349 0.46 +0.2 144,917 + GFF2349 0.65 +0.3 144,978 + GFF2349 0.75 -0.2 144,978 + GFF2349 0.75 -0.1 144,979 - GFF2349 0.75 +0.7 144,982 + GFF2349 0.75 +0.7 144,982 + GFF2349 0.75 +1.3 144,982 + GFF2349 0.75 +0.3 145,089 - -0.1 145,091 + +0.1 145,119 + +0.1 145,152 + -0.5 145,181 - -0.5 145,217 - GFF2350 0.17 -1.1 145,274 - GFF2350 0.33 -0.6 145,293 + GFF2350 0.39 -0.4 145,294 - GFF2350 0.39 -0.6 145,295 + GFF2350 0.39 -0.4 145,295 + GFF2350 0.39 -0.1 145,295 + GFF2350 0.39 -0.6 145,296 - GFF2350 0.39 -1.8 145,325 - GFF2350 0.47 -0.3 145,327 + GFF2350 0.48 -1.1 145,328 - GFF2350 0.48 +0.0 145,328 - GFF2350 0.48 -0.3 145,328 - GFF2350 0.48 -0.2 145,380 + GFF2350 0.63 -0.8 145,385 + GFF2350 0.64 -0.5 145,385 + GFF2350 0.64 -0.7 145,385 + GFF2350 0.64 -0.1 145,386 - GFF2350 0.64 -0.1 145,386 - GFF2350 0.64 -0.5 145,387 + GFF2350 0.65 -0.4 145,387 + GFF2350 0.65 -0.3 145,388 - GFF2350 0.65 +0.0 145,477 + GFF2350 0.90 -0.5 145,535 + +0.7 145,535 + -1.4 145,554 + -0.6 145,555 - -0.9 145,593 - +0.1 145,601 + -1.2 145,601 + +0.6 145,603 + -0.2 145,706 + GFF2351 0.13 -0.3 145,912 + GFF2351 0.32 -0.6 146,008 + GFF2351 0.41 -2.5 146,009 - GFF2351 0.41 -0.5 146,072 + GFF2351 0.47 -0.2 146,120 - GFF2351 0.51 -0.1 146,160 - GFF2351 0.54 -0.3 146,160 - GFF2351 0.54 -0.9 146,285 + GFF2351 0.66 -0.9 146,290 + GFF2351 0.66 -0.9 146,332 + GFF2351 0.70 -0.7 146,352 - GFF2351 0.72 -1.4 146,405 + GFF2351 0.77 +0.0 146,474 + GFF2351 0.83 +0.7 146,474 + GFF2351 0.83 -0.5 146,474 + GFF2351 0.83 -0.3 146,475 - GFF2351 0.83 +0.0 146,482 + GFF2351 0.84 -0.1 146,526 - GFF2351 0.88 -0.4 146,526 - GFF2351 0.88 -1.5 146,554 - -0.3 146,588 + -1.0 146,589 - -1.3 146,629 + +0.0 146,629 + -0.4 146,630 - -0.5 146,630 - +0.3 146,630 - -0.5 146,630 - -0.2 146,630 - -0.0 146,723 - -0.2 146,805 + -1.1 146,805 + +0.0 146,806 - -0.7 146,806 - -0.3 146,871 - -0.2 146,871 - -0.1 146,985 - -0.5 147,015 - -0.5 147,015 - +0.3 147,016 + -1.7 147,016 + -0.3 147,320 + GFF2352 0.16 +0.5 147,320 + GFF2352 0.16 -0.3 147,321 - GFF2352 0.16 -0.3 147,321 - GFF2352 0.16 -1.6 147,418 + GFF2352 0.21 -1.4 147,418 + GFF2352 0.21 -0.3 147,418 + GFF2352 0.21 -1.7 147,477 + GFF2352 0.24 -1.8 147,483 + GFF2352 0.24 -0.2 147,534 + GFF2352 0.27 -1.1 147,534 + GFF2352 0.27 -1.0
Or see this region's nucleotide sequence