Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2344 and GFF2345 are separated by 10 nucleotides GFF2345 and GFF2346 are separated by 117 nucleotides GFF2346 and GFF2347 are separated by 284 nucleotides
GFF2344 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 138,437 to 139,441
GFF2344
GFF2345 - Acetylglutamate kinase (EC 2.7.2.8), at 139,452 to 140,225
GFF2345
GFF2346 - Argininosuccinate lyase (EC 4.3.2.1), at 140,343 to 141,719
GFF2346
GFF2347 - Hydrogen peroxide-inducible genes activator, at 142,004 to 142,921
GFF2347
Position (kb)
140
141
142 Strain fitness (log2 ratio)
-2
-1
0
1 at 139.400 kb on - strand at 139.435 kb on + strand at 139.436 kb on - strand at 139.466 kb on + strand at 139.483 kb on + strand at 139.486 kb on + strand at 139.486 kb on + strand at 139.537 kb on - strand, within GFF2345 at 139.700 kb on + strand, within GFF2345 at 140.075 kb on + strand, within GFF2345 at 140.075 kb on + strand, within GFF2345 at 140.075 kb on + strand, within GFF2345 at 140.079 kb on - strand, within GFF2345 at 140.224 kb on - strand at 140.224 kb on - strand at 140.228 kb on - strand at 140.228 kb on - strand at 140.266 kb on - strand at 140.266 kb on - strand at 140.339 kb on + strand at 140.340 kb on - strand at 140.366 kb on + strand at 140.442 kb on - strand at 140.591 kb on + strand, within GFF2346 at 140.597 kb on + strand, within GFF2346 at 140.802 kb on + strand, within GFF2346 at 140.804 kb on + strand, within GFF2346 at 140.804 kb on + strand, within GFF2346 at 140.805 kb on - strand, within GFF2346 at 140.856 kb on + strand, within GFF2346 at 140.904 kb on - strand, within GFF2346 at 140.996 kb on + strand, within GFF2346 at 140.997 kb on - strand, within GFF2346 at 141.143 kb on - strand, within GFF2346 at 141.238 kb on + strand, within GFF2346 at 141.292 kb on - strand, within GFF2346 at 141.293 kb on + strand, within GFF2346 at 141.500 kb on + strand, within GFF2346 at 141.500 kb on + strand, within GFF2346 at 141.500 kb on + strand, within GFF2346 at 141.500 kb on + strand, within GFF2346 at 141.520 kb on + strand, within GFF2346 at 141.528 kb on - strand, within GFF2346 at 141.540 kb on - strand, within GFF2346 at 141.718 kb on - strand at 141.718 kb on - strand at 141.724 kb on + strand at 141.724 kb on + strand at 141.725 kb on - strand at 141.726 kb on + strand at 141.727 kb on - strand at 141.732 kb on + strand at 141.835 kb on - strand at 141.917 kb on - strand at 141.959 kb on - strand at 141.964 kb on - strand at 141.964 kb on - strand at 142.025 kb on + strand at 142.111 kb on - strand, within GFF2347 at 142.115 kb on - strand, within GFF2347 at 142.189 kb on - strand, within GFF2347 at 142.216 kb on - strand, within GFF2347 at 142.328 kb on - strand, within GFF2347 at 142.370 kb on + strand, within GFF2347 at 142.371 kb on - strand, within GFF2347 at 142.435 kb on + strand, within GFF2347 at 142.712 kb on - strand, within GFF2347 at 142.714 kb on + strand, within GFF2347 at 142.714 kb on + strand, within GFF2347
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 139,400 - -0.3 139,435 + -1.0 139,436 - -0.2 139,466 + -1.5 139,483 + -0.5 139,486 + -0.6 139,486 + -0.3 139,537 - GFF2345 0.11 -0.9 139,700 + GFF2345 0.32 +0.1 140,075 + GFF2345 0.80 +0.4 140,075 + GFF2345 0.80 -0.2 140,075 + GFF2345 0.80 -0.3 140,079 - GFF2345 0.81 +1.5 140,224 - -0.4 140,224 - -0.5 140,228 - -0.5 140,228 - -1.0 140,266 - +0.1 140,266 - +0.2 140,339 + -2.2 140,340 - -0.2 140,366 + -0.9 140,442 - -0.8 140,591 + GFF2346 0.18 -0.4 140,597 + GFF2346 0.18 -0.8 140,802 + GFF2346 0.33 -0.2 140,804 + GFF2346 0.33 -1.6 140,804 + GFF2346 0.33 -0.5 140,805 - GFF2346 0.34 -0.3 140,856 + GFF2346 0.37 -0.5 140,904 - GFF2346 0.41 -1.3 140,996 + GFF2346 0.47 -0.4 140,997 - GFF2346 0.47 -0.6 141,143 - GFF2346 0.58 -0.5 141,238 + GFF2346 0.65 +0.1 141,292 - GFF2346 0.69 +0.2 141,293 + GFF2346 0.69 +0.2 141,500 + GFF2346 0.84 +0.2 141,500 + GFF2346 0.84 -0.5 141,500 + GFF2346 0.84 +0.0 141,500 + GFF2346 0.84 -0.3 141,520 + GFF2346 0.85 -0.5 141,528 - GFF2346 0.86 -0.5 141,540 - GFF2346 0.87 -0.6 141,718 - -0.4 141,718 - +1.5 141,724 + -0.3 141,724 + -0.5 141,725 - +0.2 141,726 + -0.5 141,727 - -0.2 141,732 + -0.4 141,835 - +0.1 141,917 - -0.0 141,959 - +0.1 141,964 - -0.3 141,964 - +0.5 142,025 + -0.3 142,111 - GFF2347 0.12 -0.4 142,115 - GFF2347 0.12 -0.6 142,189 - GFF2347 0.20 +0.6 142,216 - GFF2347 0.23 -2.3 142,328 - GFF2347 0.35 -0.3 142,370 + GFF2347 0.40 -0.2 142,371 - GFF2347 0.40 -1.5 142,435 + GFF2347 0.47 +0.0 142,712 - GFF2347 0.77 +0.0 142,714 + GFF2347 0.77 -0.4 142,714 + GFF2347 0.77 +0.1
Or see this region's nucleotide sequence