Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF206 and GFF207 are separated by 34 nucleotides GFF207 and GFF208 are separated by 57 nucleotides
GFF206 - L-carnitine/gamma-butyrobetaine antiporter, at 200,887 to 202,404
GFF206
GFF207 - Crotonobetainyl-CoA dehydrogenase (EC 1.3.99.-), at 202,439 to 203,581
GFF207
GFF208 - Crotonobetainyl-CoA:carnitine CoA-transferase (EC 2.8.3.-), at 203,639 to 204,910
GFF208
Position (kb) 202
203
204 Strain fitness (log2 ratio) -1
0
1
2 at 201.472 kb on + strand, within GFF206 at 201.473 kb on - strand, within GFF206 at 201.540 kb on + strand, within GFF206 at 201.541 kb on - strand, within GFF206 at 201.568 kb on - strand, within GFF206 at 201.673 kb on - strand, within GFF206 at 201.676 kb on + strand, within GFF206 at 201.676 kb on + strand, within GFF206 at 201.739 kb on + strand, within GFF206 at 201.739 kb on + strand, within GFF206 at 201.740 kb on - strand, within GFF206 at 201.740 kb on - strand, within GFF206 at 201.794 kb on - strand, within GFF206 at 201.918 kb on - strand, within GFF206 at 202.168 kb on + strand, within GFF206 at 202.227 kb on - strand, within GFF206 at 202.351 kb on - strand at 202.403 kb on - strand at 202.409 kb on + strand at 202.520 kb on + strand at 202.520 kb on + strand at 202.520 kb on + strand at 202.551 kb on - strand at 202.701 kb on - strand, within GFF207 at 202.807 kb on + strand, within GFF207 at 202.808 kb on - strand, within GFF207 at 202.841 kb on - strand, within GFF207 at 202.841 kb on - strand, within GFF207 at 202.857 kb on - strand, within GFF207 at 202.894 kb on + strand, within GFF207 at 202.904 kb on - strand, within GFF207 at 202.919 kb on + strand, within GFF207 at 202.919 kb on + strand, within GFF207 at 202.922 kb on - strand, within GFF207 at 202.928 kb on + strand, within GFF207 at 202.929 kb on - strand, within GFF207 at 203.183 kb on + strand, within GFF207 at 203.183 kb on + strand, within GFF207 at 203.184 kb on - strand, within GFF207 at 203.393 kb on + strand, within GFF207 at 203.602 kb on + strand at 203.603 kb on - strand at 203.662 kb on - strand at 203.865 kb on - strand, within GFF208 at 204.077 kb on + strand, within GFF208 at 204.110 kb on + strand, within GFF208 at 204.110 kb on + strand, within GFF208 at 204.111 kb on - strand, within GFF208 at 204.188 kb on + strand, within GFF208 at 204.200 kb on + strand, within GFF208 at 204.293 kb on + strand, within GFF208 at 204.321 kb on - strand, within GFF208 at 204.365 kb on - strand, within GFF208 at 204.390 kb on - strand, within GFF208 at 204.408 kb on - strand, within GFF208 at 204.425 kb on + strand, within GFF208
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 201,472 + GFF206 0.39 +0.2 201,473 - GFF206 0.39 -0.1 201,540 + GFF206 0.43 -0.3 201,541 - GFF206 0.43 -0.1 201,568 - GFF206 0.45 +2.6 201,673 - GFF206 0.52 +1.0 201,676 + GFF206 0.52 +0.2 201,676 + GFF206 0.52 -0.3 201,739 + GFF206 0.56 +0.8 201,739 + GFF206 0.56 +0.5 201,740 - GFF206 0.56 +0.5 201,740 - GFF206 0.56 +0.8 201,794 - GFF206 0.60 +0.4 201,918 - GFF206 0.68 -0.3 202,168 + GFF206 0.84 +0.5 202,227 - GFF206 0.88 +0.1 202,351 - +0.7 202,403 - -0.5 202,409 + +0.6 202,520 + +1.0 202,520 + -0.7 202,520 + -0.0 202,551 - +0.1 202,701 - GFF207 0.23 -0.2 202,807 + GFF207 0.32 -0.4 202,808 - GFF207 0.32 +0.1 202,841 - GFF207 0.35 +1.8 202,841 - GFF207 0.35 +0.6 202,857 - GFF207 0.37 +0.5 202,894 + GFF207 0.40 +0.3 202,904 - GFF207 0.41 -0.3 202,919 + GFF207 0.42 -0.5 202,919 + GFF207 0.42 -0.3 202,922 - GFF207 0.42 +0.6 202,928 + GFF207 0.43 +0.1 202,929 - GFF207 0.43 +0.1 203,183 + GFF207 0.65 +0.5 203,183 + GFF207 0.65 +1.1 203,184 - GFF207 0.65 +2.3 203,393 + GFF207 0.83 +0.0 203,602 + -0.2 203,603 - +0.6 203,662 - -0.7 203,865 - GFF208 0.18 -0.9 204,077 + GFF208 0.34 +0.2 204,110 + GFF208 0.37 +0.1 204,110 + GFF208 0.37 +0.2 204,111 - GFF208 0.37 +0.4 204,188 + GFF208 0.43 +0.3 204,200 + GFF208 0.44 +0.5 204,293 + GFF208 0.51 +1.0 204,321 - GFF208 0.54 +0.0 204,365 - GFF208 0.57 +0.2 204,390 - GFF208 0.59 +1.3 204,408 - GFF208 0.60 -0.0 204,425 + GFF208 0.62 +0.5
Or see this region's nucleotide sequence