Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1887 and GFF1888 overlap by 4 nucleotides GFF1888 and GFF1889 are separated by 2 nucleotides GFF1889 and GFF1890 are separated by 86 nucleotides GFF1890 and GFF1891 are separated by 15 nucleotides
GFF1887 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 81,044 to 82,381
GFF1887
GFF1888 - NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3), at 82,378 to 82,878
GFF1888
GFF1889 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 82,881 to 84,683
GFF1889
GFF1890 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 84,770 to 85,432
GFF1890
GFF1891 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 85,448 to 85,891
GFF1891
Position (kb)
82
83
84
85 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 81.960 kb on - strand, within GFF1887 at 81.974 kb on - strand, within GFF1887 at 81.996 kb on + strand, within GFF1887 at 81.997 kb on - strand, within GFF1887 at 81.997 kb on - strand, within GFF1887 at 82.036 kb on + strand, within GFF1887 at 82.036 kb on + strand, within GFF1887 at 82.037 kb on - strand, within GFF1887 at 82.086 kb on + strand, within GFF1887 at 82.653 kb on - strand, within GFF1888 at 82.653 kb on - strand, within GFF1888 at 82.712 kb on + strand, within GFF1888 at 82.813 kb on + strand, within GFF1888 at 82.814 kb on - strand, within GFF1888 at 82.882 kb on + strand at 82.901 kb on + strand at 83.021 kb on + strand at 83.022 kb on - strand at 83.045 kb on - strand at 83.047 kb on + strand at 83.226 kb on - strand, within GFF1889 at 83.293 kb on + strand, within GFF1889 at 83.293 kb on + strand, within GFF1889 at 83.293 kb on + strand, within GFF1889 at 83.293 kb on + strand, within GFF1889 at 83.294 kb on - strand, within GFF1889 at 83.366 kb on + strand, within GFF1889 at 83.444 kb on - strand, within GFF1889 at 83.477 kb on + strand, within GFF1889 at 83.824 kb on + strand, within GFF1889 at 83.855 kb on - strand, within GFF1889 at 83.871 kb on - strand, within GFF1889 at 83.881 kb on + strand, within GFF1889 at 83.881 kb on + strand, within GFF1889 at 83.882 kb on - strand, within GFF1889 at 83.886 kb on - strand, within GFF1889 at 83.886 kb on - strand, within GFF1889 at 83.886 kb on - strand, within GFF1889 at 83.985 kb on - strand, within GFF1889 at 83.985 kb on - strand, within GFF1889 at 84.038 kb on + strand, within GFF1889 at 84.039 kb on - strand, within GFF1889 at 84.039 kb on - strand, within GFF1889 at 84.274 kb on + strand, within GFF1889 at 84.354 kb on - strand, within GFF1889 at 84.493 kb on - strand, within GFF1889 at 84.660 kb on + strand at 84.937 kb on + strand, within GFF1890 at 84.938 kb on - strand, within GFF1890 at 84.940 kb on - strand, within GFF1890 at 84.972 kb on + strand, within GFF1890 at 84.972 kb on + strand, within GFF1890 at 84.972 kb on + strand, within GFF1890 at 84.973 kb on - strand, within GFF1890 at 84.973 kb on - strand, within GFF1890 at 84.973 kb on - strand, within GFF1890 at 84.994 kb on + strand, within GFF1890 at 84.994 kb on + strand, within GFF1890 at 85.027 kb on - strand, within GFF1890 at 85.093 kb on - strand, within GFF1890 at 85.235 kb on + strand, within GFF1890 at 85.235 kb on + strand, within GFF1890 at 85.236 kb on - strand, within GFF1890 at 85.239 kb on + strand, within GFF1890 at 85.240 kb on - strand, within GFF1890 at 85.240 kb on - strand, within GFF1890 at 85.410 kb on - strand at 85.435 kb on + strand at 85.435 kb on + strand at 85.545 kb on + strand, within GFF1891 at 85.641 kb on + strand, within GFF1891
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 81,960 - GFF1887 0.68 -0.8 81,974 - GFF1887 0.70 +0.3 81,996 + GFF1887 0.71 +0.1 81,997 - GFF1887 0.71 -0.4 81,997 - GFF1887 0.71 +0.1 82,036 + GFF1887 0.74 -0.0 82,036 + GFF1887 0.74 -0.8 82,037 - GFF1887 0.74 -0.7 82,086 + GFF1887 0.78 -0.1 82,653 - GFF1888 0.55 -0.2 82,653 - GFF1888 0.55 -1.0 82,712 + GFF1888 0.67 +1.8 82,813 + GFF1888 0.87 -0.6 82,814 - GFF1888 0.87 +0.7 82,882 + +0.2 82,901 + +0.1 83,021 + -0.5 83,022 - +0.1 83,045 - -0.5 83,047 + -0.3 83,226 - GFF1889 0.19 -0.9 83,293 + GFF1889 0.23 -0.7 83,293 + GFF1889 0.23 +0.2 83,293 + GFF1889 0.23 -2.0 83,293 + GFF1889 0.23 -1.4 83,294 - GFF1889 0.23 -0.8 83,366 + GFF1889 0.27 -0.6 83,444 - GFF1889 0.31 +0.0 83,477 + GFF1889 0.33 +0.1 83,824 + GFF1889 0.52 -0.4 83,855 - GFF1889 0.54 -0.9 83,871 - GFF1889 0.55 -2.1 83,881 + GFF1889 0.55 -1.3 83,881 + GFF1889 0.55 -0.5 83,882 - GFF1889 0.56 -0.3 83,886 - GFF1889 0.56 +0.8 83,886 - GFF1889 0.56 -0.5 83,886 - GFF1889 0.56 -0.8 83,985 - GFF1889 0.61 -0.1 83,985 - GFF1889 0.61 -2.6 84,038 + GFF1889 0.64 -1.1 84,039 - GFF1889 0.64 -0.6 84,039 - GFF1889 0.64 -0.4 84,274 + GFF1889 0.77 -0.9 84,354 - GFF1889 0.82 -0.3 84,493 - GFF1889 0.89 +0.2 84,660 + -0.8 84,937 + GFF1890 0.25 -0.7 84,938 - GFF1890 0.25 -0.2 84,940 - GFF1890 0.26 +0.2 84,972 + GFF1890 0.30 -0.3 84,972 + GFF1890 0.30 -0.8 84,972 + GFF1890 0.30 -0.9 84,973 - GFF1890 0.31 -0.3 84,973 - GFF1890 0.31 -2.3 84,973 - GFF1890 0.31 +0.0 84,994 + GFF1890 0.34 +0.1 84,994 + GFF1890 0.34 -0.4 85,027 - GFF1890 0.39 +0.1 85,093 - GFF1890 0.49 -0.1 85,235 + GFF1890 0.70 -1.2 85,235 + GFF1890 0.70 +0.7 85,236 - GFF1890 0.70 -2.3 85,239 + GFF1890 0.71 -0.7 85,240 - GFF1890 0.71 -0.0 85,240 - GFF1890 0.71 -0.9 85,410 - -0.7 85,435 + +0.2 85,435 + -0.4 85,545 + GFF1891 0.22 -0.0 85,641 + GFF1891 0.43 -0.4
Or see this region's nucleotide sequence