Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1870 and GFF1871 are separated by 85 nucleotides GFF1871 and GFF1872 are separated by 100 nucleotides GFF1872 and GFF1873 are separated by 54 nucleotides
GFF1870 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase (EC 2.2.1.9), at 62,019 to 63,689
GFF1870
GFF1871 - Menaquinone-specific isochorismate synthase (EC 5.4.4.2), at 63,775 to 65,070
GFF1871
GFF1872 - ElaB protein, at 65,171 to 65,476
GFF1872
GFF1873 - ElaA protein, at 65,531 to 65,992
GFF1873
Position (kb) 63
64
65
66 Strain fitness (log2 ratio) -1
0
1
2
3 at 62.828 kb on + strand, within GFF1870 at 62.935 kb on - strand, within GFF1870 at 63.167 kb on - strand, within GFF1870 at 63.548 kb on + strand at 63.745 kb on + strand at 63.799 kb on - strand at 63.824 kb on - strand at 63.916 kb on + strand, within GFF1871 at 63.917 kb on - strand, within GFF1871 at 63.953 kb on + strand, within GFF1871 at 63.954 kb on - strand, within GFF1871 at 63.971 kb on + strand, within GFF1871 at 63.971 kb on + strand, within GFF1871 at 64.107 kb on + strand, within GFF1871 at 64.219 kb on - strand, within GFF1871 at 64.275 kb on + strand, within GFF1871 at 64.276 kb on - strand, within GFF1871 at 64.478 kb on - strand, within GFF1871 at 64.623 kb on + strand, within GFF1871 at 64.624 kb on - strand, within GFF1871 at 64.699 kb on + strand, within GFF1871 at 64.726 kb on - strand, within GFF1871 at 64.753 kb on + strand, within GFF1871 at 64.754 kb on - strand, within GFF1871 at 64.754 kb on - strand, within GFF1871 at 64.757 kb on + strand, within GFF1871 at 64.855 kb on + strand, within GFF1871 at 64.910 kb on + strand, within GFF1871 at 65.061 kb on + strand at 65.138 kb on - strand at 65.228 kb on - strand, within GFF1872 at 65.289 kb on + strand, within GFF1872 at 65.296 kb on - strand, within GFF1872 at 65.371 kb on - strand, within GFF1872 at 65.403 kb on - strand, within GFF1872 at 65.470 kb on - strand at 65.508 kb on + strand at 65.509 kb on - strand at 65.512 kb on + strand at 65.513 kb on - strand at 65.513 kb on - strand at 65.655 kb on + strand, within GFF1873 at 65.655 kb on + strand, within GFF1873 at 65.657 kb on + strand, within GFF1873 at 65.843 kb on - strand, within GFF1873 at 65.862 kb on - strand, within GFF1873
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 62,828 + GFF1870 0.48 +0.3 62,935 - GFF1870 0.55 -0.0 63,167 - GFF1870 0.69 -0.6 63,548 + -0.1 63,745 + -0.1 63,799 - -0.1 63,824 - +0.2 63,916 + GFF1871 0.11 +2.4 63,917 - GFF1871 0.11 -0.3 63,953 + GFF1871 0.14 -1.5 63,954 - GFF1871 0.14 +0.9 63,971 + GFF1871 0.15 +1.0 63,971 + GFF1871 0.15 +0.9 64,107 + GFF1871 0.26 +3.2 64,219 - GFF1871 0.34 -0.1 64,275 + GFF1871 0.39 +1.0 64,276 - GFF1871 0.39 +1.2 64,478 - GFF1871 0.54 +0.0 64,623 + GFF1871 0.65 +0.9 64,624 - GFF1871 0.66 +0.2 64,699 + GFF1871 0.71 +0.3 64,726 - GFF1871 0.73 -0.3 64,753 + GFF1871 0.75 +0.5 64,754 - GFF1871 0.76 +0.9 64,754 - GFF1871 0.76 -0.3 64,757 + GFF1871 0.76 -0.3 64,855 + GFF1871 0.83 -0.8 64,910 + GFF1871 0.88 +0.4 65,061 + -0.1 65,138 - +0.3 65,228 - GFF1872 0.19 -0.5 65,289 + GFF1872 0.39 +0.3 65,296 - GFF1872 0.41 +0.2 65,371 - GFF1872 0.65 -0.1 65,403 - GFF1872 0.76 -0.4 65,470 - +1.2 65,508 + -0.1 65,509 - +0.8 65,512 + -0.1 65,513 - +0.1 65,513 - -0.1 65,655 + GFF1873 0.27 +0.3 65,655 + GFF1873 0.27 +0.2 65,657 + GFF1873 0.27 +0.3 65,843 - GFF1873 0.68 +1.1 65,862 - GFF1873 0.72 +0.3
Or see this region's nucleotide sequence