Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1500 and GFF1501 are separated by 150 nucleotides GFF1501 and GFF1502 are separated by 211 nucleotides GFF1502 and GFF1503 are separated by 109 nucleotides
GFF1500 - tRNA pseudouridine synthase B (EC 4.2.1.70), at 5,570 to 6,514
GFF1500
GFF1501 - SSU ribosomal protein S15p (S13e), at 6,665 to 6,934
GFF1501
GFF1502 - Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), at 7,146 to 9,311
GFF1502
GFF1503 - Lipoprotein nlpI precursor, at 9,421 to 10,305
GFF1503
Position (kb)
7
8
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6.159 kb on + strand, within GFF1500 at 6.159 kb on + strand, within GFF1500 at 6.160 kb on - strand, within GFF1500 at 6.164 kb on + strand, within GFF1500 at 6.289 kb on - strand, within GFF1500 at 6.332 kb on - strand, within GFF1500 at 6.332 kb on - strand, within GFF1500 at 6.354 kb on + strand, within GFF1500 at 6.357 kb on - strand, within GFF1500 at 6.382 kb on + strand, within GFF1500 at 6.495 kb on + strand at 6.498 kb on + strand at 6.498 kb on + strand at 6.499 kb on - strand at 6.512 kb on + strand at 6.512 kb on + strand at 6.513 kb on - strand at 6.580 kb on + strand at 6.933 kb on - strand at 7.010 kb on - strand at 7.014 kb on + strand at 7.015 kb on - strand at 7.015 kb on - strand at 7.098 kb on + strand at 7.197 kb on - strand at 7.273 kb on + strand at 7.362 kb on + strand at 7.363 kb on - strand, within GFF1502 at 7.373 kb on + strand, within GFF1502 at 7.641 kb on + strand, within GFF1502 at 7.656 kb on + strand, within GFF1502 at 7.776 kb on - strand, within GFF1502 at 7.776 kb on - strand, within GFF1502 at 8.492 kb on + strand, within GFF1502 at 8.546 kb on + strand, within GFF1502 at 8.598 kb on + strand, within GFF1502 at 8.598 kb on + strand, within GFF1502 at 8.599 kb on - strand, within GFF1502 at 8.679 kb on - strand, within GFF1502 at 8.679 kb on - strand, within GFF1502 at 8.714 kb on + strand, within GFF1502 at 8.816 kb on + strand, within GFF1502 at 8.816 kb on + strand, within GFF1502 at 8.843 kb on + strand, within GFF1502 at 8.843 kb on + strand, within GFF1502 at 8.994 kb on + strand, within GFF1502 at 9.049 kb on - strand, within GFF1502 at 9.049 kb on - strand, within GFF1502 at 9.056 kb on + strand, within GFF1502 at 9.057 kb on - strand, within GFF1502 at 9.229 kb on + strand at 9.242 kb on + strand at 9.309 kb on + strand at 9.346 kb on + strand at 9.462 kb on + strand at 9.462 kb on + strand at 9.462 kb on + strand at 9.463 kb on - strand at 9.463 kb on - strand at 9.463 kb on - strand at 9.480 kb on + strand at 9.480 kb on + strand at 9.640 kb on - strand, within GFF1503 at 9.725 kb on + strand, within GFF1503 at 9.805 kb on + strand, within GFF1503 at 9.841 kb on + strand, within GFF1503 at 10.135 kb on + strand, within GFF1503 at 10.135 kb on + strand, within GFF1503 at 10.135 kb on + strand, within GFF1503 at 10.139 kb on + strand, within GFF1503 at 10.154 kb on + strand, within GFF1503
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 6,159 + GFF1500 0.62 +0.8 6,159 + GFF1500 0.62 +1.7 6,160 - GFF1500 0.62 -0.8 6,164 + GFF1500 0.63 +0.4 6,289 - GFF1500 0.76 -0.3 6,332 - GFF1500 0.81 +0.4 6,332 - GFF1500 0.81 -0.4 6,354 + GFF1500 0.83 +0.2 6,357 - GFF1500 0.83 +0.3 6,382 + GFF1500 0.86 +0.2 6,495 + -0.3 6,498 + +0.9 6,498 + +0.8 6,499 - -0.6 6,512 + -0.0 6,512 + +0.2 6,513 - +0.1 6,580 + -0.5 6,933 - -1.9 7,010 - -0.2 7,014 + -0.3 7,015 - -0.8 7,015 - -2.4 7,098 + -1.1 7,197 - -2.0 7,273 + -0.3 7,362 + +0.1 7,363 - GFF1502 0.10 -1.2 7,373 + GFF1502 0.10 -1.3 7,641 + GFF1502 0.23 -2.8 7,656 + GFF1502 0.24 -1.5 7,776 - GFF1502 0.29 -2.9 7,776 - GFF1502 0.29 -2.2 8,492 + GFF1502 0.62 -1.4 8,546 + GFF1502 0.65 -3.7 8,598 + GFF1502 0.67 -1.0 8,598 + GFF1502 0.67 -2.1 8,599 - GFF1502 0.67 -1.9 8,679 - GFF1502 0.71 -0.9 8,679 - GFF1502 0.71 -1.6 8,714 + GFF1502 0.72 -0.9 8,816 + GFF1502 0.77 +0.2 8,816 + GFF1502 0.77 -0.7 8,843 + GFF1502 0.78 -0.6 8,843 + GFF1502 0.78 -3.2 8,994 + GFF1502 0.85 +2.8 9,049 - GFF1502 0.88 -1.6 9,049 - GFF1502 0.88 -1.0 9,056 + GFF1502 0.88 +1.1 9,057 - GFF1502 0.88 -0.6 9,229 + +1.4 9,242 + -1.2 9,309 + +0.2 9,346 + -0.1 9,462 + -1.0 9,462 + -1.0 9,462 + -0.7 9,463 - -1.5 9,463 - -1.9 9,463 - -1.3 9,480 + +0.4 9,480 + -1.0 9,640 - GFF1503 0.25 -1.2 9,725 + GFF1503 0.34 -1.2 9,805 + GFF1503 0.43 +0.4 9,841 + GFF1503 0.47 -0.1 10,135 + GFF1503 0.81 +0.4 10,135 + GFF1503 0.81 -1.5 10,135 + GFF1503 0.81 -1.0 10,139 + GFF1503 0.81 -3.0 10,154 + GFF1503 0.83 -3.3
Or see this region's nucleotide sequence