Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1008 and GFF1009 are separated by 465 nucleotides GFF1009 and GFF1010 are separated by 112 nucleotides GFF1010 and GFF1011 are separated by 61 nucleotides
GFF1008 - Carbon starvation protein A paralog, at 190,917 to 192,968
GFF1008
GFF1009 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 193,434 to 195,095
GFF1009
GFF1010 - FIG01048313: hypothetical protein, at 195,208 to 195,357
GFF1010
GFF1011 - Transcriptional regulatory protein levR, at 195,419 to 198,184
GFF1011
Position (kb)
193
194
195
196 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 192.437 kb on - strand, within GFF1008 at 192.437 kb on - strand, within GFF1008 at 192.555 kb on + strand, within GFF1008 at 192.555 kb on + strand, within GFF1008 at 192.555 kb on + strand, within GFF1008 at 192.898 kb on + strand at 192.899 kb on - strand at 192.907 kb on + strand at 192.908 kb on - strand at 193.039 kb on - strand at 193.071 kb on - strand at 193.089 kb on + strand at 193.138 kb on - strand at 193.149 kb on + strand at 193.149 kb on + strand at 193.150 kb on - strand at 193.150 kb on - strand at 193.191 kb on - strand at 193.194 kb on + strand at 193.223 kb on - strand at 193.297 kb on + strand at 193.331 kb on + strand at 193.349 kb on - strand at 193.358 kb on - strand at 193.409 kb on + strand at 193.409 kb on + strand at 193.409 kb on + strand at 193.755 kb on + strand, within GFF1009 at 193.818 kb on + strand, within GFF1009 at 193.818 kb on + strand, within GFF1009 at 193.818 kb on + strand, within GFF1009 at 193.818 kb on + strand, within GFF1009 at 193.819 kb on - strand, within GFF1009 at 193.819 kb on - strand, within GFF1009 at 193.819 kb on - strand, within GFF1009 at 193.823 kb on + strand, within GFF1009 at 193.823 kb on + strand, within GFF1009 at 193.908 kb on + strand, within GFF1009 at 193.932 kb on + strand, within GFF1009 at 193.932 kb on + strand, within GFF1009 at 193.932 kb on + strand, within GFF1009 at 193.932 kb on + strand, within GFF1009 at 193.933 kb on - strand, within GFF1009 at 193.941 kb on + strand, within GFF1009 at 193.942 kb on - strand, within GFF1009 at 193.943 kb on + strand, within GFF1009 at 193.943 kb on + strand, within GFF1009 at 193.943 kb on + strand, within GFF1009 at 193.965 kb on + strand, within GFF1009 at 193.965 kb on + strand, within GFF1009 at 193.998 kb on + strand, within GFF1009 at 193.998 kb on + strand, within GFF1009 at 194.584 kb on + strand, within GFF1009 at 194.722 kb on + strand, within GFF1009 at 194.723 kb on - strand, within GFF1009 at 194.723 kb on - strand, within GFF1009 at 195.121 kb on - strand at 195.210 kb on - strand at 195.246 kb on - strand, within GFF1010 at 195.386 kb on + strand at 195.386 kb on + strand at 195.386 kb on + strand at 195.387 kb on - strand at 195.523 kb on + strand at 195.523 kb on + strand at 195.524 kb on - strand at 195.917 kb on - strand, within GFF1011 at 195.917 kb on - strand, within GFF1011 at 196.053 kb on + strand, within GFF1011 at 196.053 kb on + strand, within GFF1011 at 196.054 kb on - strand, within GFF1011
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 192,437 - GFF1008 0.74 -0.1 192,437 - GFF1008 0.74 +0.4 192,555 + GFF1008 0.80 +1.4 192,555 + GFF1008 0.80 -0.1 192,555 + GFF1008 0.80 +0.3 192,898 + -0.5 192,899 - -0.5 192,907 + +0.3 192,908 - -0.8 193,039 - -0.2 193,071 - -0.5 193,089 + -0.8 193,138 - +0.0 193,149 + +2.1 193,149 + +0.3 193,150 - -0.6 193,150 - -0.7 193,191 - +0.8 193,194 + +1.1 193,223 - -0.5 193,297 + -0.9 193,331 + +0.3 193,349 - -0.7 193,358 - -0.6 193,409 + +0.0 193,409 + -0.5 193,409 + -0.1 193,755 + GFF1009 0.19 -0.3 193,818 + GFF1009 0.23 -1.2 193,818 + GFF1009 0.23 +1.1 193,818 + GFF1009 0.23 -1.6 193,818 + GFF1009 0.23 -0.1 193,819 - GFF1009 0.23 -0.2 193,819 - GFF1009 0.23 -0.8 193,819 - GFF1009 0.23 -0.2 193,823 + GFF1009 0.23 -1.9 193,823 + GFF1009 0.23 -0.1 193,908 + GFF1009 0.29 +0.2 193,932 + GFF1009 0.30 +0.2 193,932 + GFF1009 0.30 -0.3 193,932 + GFF1009 0.30 -0.7 193,932 + GFF1009 0.30 -0.2 193,933 - GFF1009 0.30 -0.1 193,941 + GFF1009 0.31 -1.3 193,942 - GFF1009 0.31 -0.7 193,943 + GFF1009 0.31 -0.5 193,943 + GFF1009 0.31 +0.2 193,943 + GFF1009 0.31 -0.5 193,965 + GFF1009 0.32 +0.8 193,965 + GFF1009 0.32 -0.4 193,998 + GFF1009 0.34 +0.1 193,998 + GFF1009 0.34 -0.6 194,584 + GFF1009 0.69 -0.1 194,722 + GFF1009 0.77 -0.0 194,723 - GFF1009 0.78 +0.5 194,723 - GFF1009 0.78 +1.0 195,121 - +0.4 195,210 - -0.6 195,246 - GFF1010 0.25 +1.0 195,386 + -0.5 195,386 + +0.3 195,386 + -0.6 195,387 - +0.4 195,523 + -0.2 195,523 + -0.4 195,524 - -0.2 195,917 - GFF1011 0.18 -0.1 195,917 - GFF1011 0.18 -0.7 196,053 + GFF1011 0.23 +0.1 196,053 + GFF1011 0.23 +0.4 196,054 - GFF1011 0.23 -0.9
Or see this region's nucleotide sequence