Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF890 and GFF891 are separated by 18 nucleotides GFF891 and GFF892 are separated by 96 nucleotides
GFF890 - Inosose dehydratase (EC 4.2.1.44), at 70,334 to 71,239
GFF890
GFF891 - Myo-inositol 2-dehydrogenase 1 (EC 1.1.1.18), at 71,258 to 72,268
GFF891
GFF892 - lysosomal glucosyl ceramidase-like protein, at 72,365 to 73,708
GFF892
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 70.317 kb on - strand at 70.337 kb on + strand at 70.358 kb on - strand at 70.583 kb on - strand, within GFF890 at 70.622 kb on + strand, within GFF890 at 70.817 kb on - strand, within GFF890 at 70.911 kb on + strand, within GFF890 at 70.912 kb on - strand, within GFF890 at 71.275 kb on + strand at 71.280 kb on + strand at 71.310 kb on + strand at 71.340 kb on + strand at 71.340 kb on + strand at 71.396 kb on - strand, within GFF891 at 71.400 kb on - strand, within GFF891 at 71.450 kb on - strand, within GFF891 at 71.525 kb on - strand, within GFF891 at 71.765 kb on - strand, within GFF891 at 71.765 kb on - strand, within GFF891 at 71.768 kb on + strand, within GFF891 at 71.769 kb on - strand, within GFF891 at 72.047 kb on - strand, within GFF891 at 72.082 kb on + strand, within GFF891 at 72.083 kb on - strand, within GFF891 at 72.170 kb on + strand at 72.462 kb on + strand at 72.463 kb on - strand at 72.663 kb on - strand, within GFF892 at 72.663 kb on - strand, within GFF892 at 72.811 kb on + strand, within GFF892 at 72.820 kb on + strand, within GFF892 at 72.893 kb on + strand, within GFF892 at 72.894 kb on - strand, within GFF892 at 72.986 kb on + strand, within GFF892 at 72.986 kb on + strand, within GFF892 at 72.987 kb on - strand, within GFF892 at 72.988 kb on + strand, within GFF892 at 72.989 kb on - strand, within GFF892 at 73.070 kb on + strand, within GFF892 at 73.172 kb on + strand, within GFF892 at 73.197 kb on + strand, within GFF892
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 70,317 - -0.0 70,337 + +0.4 70,358 - +0.1 70,583 - GFF890 0.27 -0.0 70,622 + GFF890 0.32 -1.1 70,817 - GFF890 0.53 +0.2 70,911 + GFF890 0.64 +0.5 70,912 - GFF890 0.64 -1.3 71,275 + -1.4 71,280 + +0.6 71,310 + -0.1 71,340 + +0.3 71,340 + +0.5 71,396 - GFF891 0.14 -0.3 71,400 - GFF891 0.14 +1.0 71,450 - GFF891 0.19 -0.6 71,525 - GFF891 0.26 -0.1 71,765 - GFF891 0.50 -0.5 71,765 - GFF891 0.50 +0.2 71,768 + GFF891 0.50 -1.0 71,769 - GFF891 0.51 -0.4 72,047 - GFF891 0.78 -1.3 72,082 + GFF891 0.82 +0.1 72,083 - GFF891 0.82 -0.8 72,170 + +0.1 72,462 + +3.3 72,463 - -0.5 72,663 - GFF892 0.22 -0.1 72,663 - GFF892 0.22 -0.3 72,811 + GFF892 0.33 +0.5 72,820 + GFF892 0.34 -0.1 72,893 + GFF892 0.39 -0.3 72,894 - GFF892 0.39 -0.2 72,986 + GFF892 0.46 +3.5 72,986 + GFF892 0.46 -1.9 72,987 - GFF892 0.46 -0.9 72,988 + GFF892 0.46 +0.2 72,989 - GFF892 0.46 -0.8 73,070 + GFF892 0.52 +0.7 73,172 + GFF892 0.60 -0.6 73,197 + GFF892 0.62 +0.4
Or see this region's nucleotide sequence