Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4749 and GFF4750 are separated by 166 nucleotides GFF4750 and GFF4751 are separated by 27 nucleotides
GFF4749 - Type III secretion host injection protein (YopB), at 56,538 to 57,473
GFF4749
GFF4750 - Type III secretion negative modulator of injection (YopK,YopQ,controls size of translocator pore), at 57,640 to 58,869
GFF4750
GFF4751 - cell invasion protein SipB, at 58,897 to 60,678
GFF4751
Position (kb) 57
58
59 Strain fitness (log2 ratio) -1
0
1 at 56.787 kb on + strand, within GFF4749 at 57.036 kb on + strand, within GFF4749 at 57.037 kb on - strand, within GFF4749 at 57.125 kb on - strand, within GFF4749 at 57.144 kb on + strand, within GFF4749 at 57.146 kb on + strand, within GFF4749 at 57.222 kb on + strand, within GFF4749 at 57.570 kb on - strand at 57.572 kb on + strand at 57.624 kb on + strand at 57.624 kb on + strand at 57.625 kb on - strand at 57.641 kb on + strand at 57.694 kb on - strand at 57.742 kb on - strand at 57.933 kb on + strand, within GFF4750 at 57.934 kb on - strand, within GFF4750 at 57.934 kb on - strand, within GFF4750 at 58.078 kb on - strand, within GFF4750 at 58.128 kb on + strand, within GFF4750 at 58.129 kb on - strand, within GFF4750 at 58.177 kb on - strand, within GFF4750 at 58.177 kb on - strand, within GFF4750 at 58.200 kb on + strand, within GFF4750 at 58.203 kb on + strand, within GFF4750 at 58.228 kb on - strand, within GFF4750 at 58.281 kb on + strand, within GFF4750 at 58.412 kb on - strand, within GFF4750 at 58.468 kb on - strand, within GFF4750 at 58.681 kb on + strand, within GFF4750 at 59.169 kb on + strand, within GFF4751 at 59.182 kb on - strand, within GFF4751 at 59.182 kb on - strand, within GFF4751 at 59.185 kb on + strand, within GFF4751 at 59.197 kb on - strand, within GFF4751 at 59.251 kb on - strand, within GFF4751 at 59.704 kb on + strand, within GFF4751 at 59.739 kb on + strand, within GFF4751
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 56,787 + GFF4749 0.27 -1.0 57,036 + GFF4749 0.53 -0.3 57,037 - GFF4749 0.53 +0.7 57,125 - GFF4749 0.63 +1.3 57,144 + GFF4749 0.65 +0.3 57,146 + GFF4749 0.65 -0.2 57,222 + GFF4749 0.73 -1.7 57,570 - -0.1 57,572 + -1.5 57,624 + -0.3 57,624 + +0.3 57,625 - +0.0 57,641 + +0.0 57,694 - -0.0 57,742 - +0.1 57,933 + GFF4750 0.24 +0.0 57,934 - GFF4750 0.24 +0.4 57,934 - GFF4750 0.24 -0.5 58,078 - GFF4750 0.36 +0.1 58,128 + GFF4750 0.40 -0.4 58,129 - GFF4750 0.40 -0.1 58,177 - GFF4750 0.44 -1.7 58,177 - GFF4750 0.44 -0.4 58,200 + GFF4750 0.46 -0.2 58,203 + GFF4750 0.46 -0.6 58,228 - GFF4750 0.48 -0.0 58,281 + GFF4750 0.52 -0.3 58,412 - GFF4750 0.63 -0.3 58,468 - GFF4750 0.67 -0.0 58,681 + GFF4750 0.85 +0.0 59,169 + GFF4751 0.15 +0.2 59,182 - GFF4751 0.16 +0.6 59,182 - GFF4751 0.16 -0.1 59,185 + GFF4751 0.16 -0.2 59,197 - GFF4751 0.17 -1.2 59,251 - GFF4751 0.20 +0.0 59,704 + GFF4751 0.45 -0.7 59,739 + GFF4751 0.47 +0.7
Or see this region's nucleotide sequence