Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3472 and GFF3473 are separated by 122 nucleotides GFF3473 and GFF3474 are separated by 426 nucleotides
GFF3472 - UPF0229 protein YeaH, at 222,472 to 223,758
GFF3472
GFF3473 - Serine protein kinase (prkA protein), P-loop containing, at 223,881 to 225,815
GFF3473
GFF3474 - MltA-interacting protein MipA, at 226,242 to 226,988
GFF3474
Position (kb) 223
224
225
226 Strain fitness (log2 ratio) -1
0
1
2 at 222.885 kb on - strand, within GFF3472 at 222.905 kb on + strand, within GFF3472 at 223.311 kb on + strand, within GFF3472 at 223.350 kb on + strand, within GFF3472 at 223.581 kb on + strand, within GFF3472 at 223.585 kb on - strand, within GFF3472 at 223.680 kb on + strand at 223.681 kb on - strand at 223.759 kb on - strand at 223.838 kb on - strand at 223.942 kb on + strand at 224.012 kb on + strand at 224.181 kb on - strand, within GFF3473 at 224.291 kb on - strand, within GFF3473 at 224.417 kb on - strand, within GFF3473 at 224.540 kb on + strand, within GFF3473 at 224.540 kb on + strand, within GFF3473 at 224.590 kb on + strand, within GFF3473 at 224.622 kb on - strand, within GFF3473 at 224.842 kb on + strand, within GFF3473 at 224.843 kb on - strand, within GFF3473 at 224.930 kb on + strand, within GFF3473 at 224.965 kb on + strand, within GFF3473 at 225.008 kb on + strand, within GFF3473 at 225.012 kb on - strand, within GFF3473 at 225.053 kb on + strand, within GFF3473 at 225.120 kb on - strand, within GFF3473 at 225.290 kb on + strand, within GFF3473 at 225.370 kb on - strand, within GFF3473 at 225.544 kb on + strand, within GFF3473 at 225.545 kb on - strand, within GFF3473 at 225.545 kb on - strand, within GFF3473 at 225.665 kb on + strand at 225.688 kb on - strand at 225.809 kb on + strand at 225.809 kb on + strand at 225.862 kb on - strand at 225.868 kb on - strand at 225.889 kb on + strand at 225.987 kb on + strand at 225.993 kb on + strand at 226.019 kb on + strand at 226.153 kb on - strand at 226.358 kb on + strand, within GFF3474 at 226.402 kb on - strand, within GFF3474 at 226.446 kb on + strand, within GFF3474 at 226.447 kb on - strand, within GFF3474 at 226.447 kb on - strand, within GFF3474 at 226.634 kb on + strand, within GFF3474 at 226.655 kb on + strand, within GFF3474 at 226.758 kb on + strand, within GFF3474 at 226.768 kb on + strand, within GFF3474
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 222,885 - GFF3472 0.32 -0.0 222,905 + GFF3472 0.34 -0.6 223,311 + GFF3472 0.65 -0.3 223,350 + GFF3472 0.68 +2.2 223,581 + GFF3472 0.86 +0.2 223,585 - GFF3472 0.86 +0.2 223,680 + -0.1 223,681 - -0.3 223,759 - +0.2 223,838 - -0.0 223,942 + -1.6 224,012 + -0.0 224,181 - GFF3473 0.16 -0.2 224,291 - GFF3473 0.21 +0.1 224,417 - GFF3473 0.28 +0.3 224,540 + GFF3473 0.34 +0.5 224,540 + GFF3473 0.34 +0.2 224,590 + GFF3473 0.37 -0.3 224,622 - GFF3473 0.38 +0.2 224,842 + GFF3473 0.50 -0.4 224,843 - GFF3473 0.50 -0.4 224,930 + GFF3473 0.54 -0.1 224,965 + GFF3473 0.56 -0.1 225,008 + GFF3473 0.58 +1.1 225,012 - GFF3473 0.58 -0.9 225,053 + GFF3473 0.61 +1.2 225,120 - GFF3473 0.64 -0.2 225,290 + GFF3473 0.73 +0.6 225,370 - GFF3473 0.77 -0.4 225,544 + GFF3473 0.86 +0.6 225,545 - GFF3473 0.86 -0.3 225,545 - GFF3473 0.86 +0.5 225,665 + -0.2 225,688 - -0.2 225,809 + +0.0 225,809 + +0.2 225,862 - -0.9 225,868 - +1.0 225,889 + -0.3 225,987 + -0.4 225,993 + +0.2 226,019 + -0.2 226,153 - -0.2 226,358 + GFF3474 0.16 -0.3 226,402 - GFF3474 0.21 -0.5 226,446 + GFF3474 0.27 +0.2 226,447 - GFF3474 0.27 -0.2 226,447 - GFF3474 0.27 +0.0 226,634 + GFF3474 0.52 -0.4 226,655 + GFF3474 0.55 +0.9 226,758 + GFF3474 0.69 -0.3 226,768 + GFF3474 0.70 +0.7
Or see this region's nucleotide sequence