Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2722 and GFF2723 overlap by 1 nucleotides GFF2723 and GFF2724 are separated by 3 nucleotides
GFF2722 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 244,168 to 245,730
GFF2722
GFF2723 - Anthranilate synthase, amidotransferase component (EC 4.1.3.27) / Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 245,730 to 247,325
GFF2723
GFF2724 - Indole-3-glycerol phosphate synthase (EC 4.1.1.48) / Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 247,329 to 248,687
GFF2724
Position (kb) 245
246
247
248 Strain fitness (log2 ratio) -2
-1
0
1
2 at 244.812 kb on + strand, within GFF2722 at 244.823 kb on + strand, within GFF2722 at 244.914 kb on - strand, within GFF2722 at 244.950 kb on - strand, within GFF2722 at 245.009 kb on - strand, within GFF2722 at 245.012 kb on - strand, within GFF2722 at 245.012 kb on - strand, within GFF2722 at 245.012 kb on - strand, within GFF2722 at 245.042 kb on - strand, within GFF2722 at 245.042 kb on - strand, within GFF2722 at 245.043 kb on + strand, within GFF2722 at 245.044 kb on - strand, within GFF2722 at 245.182 kb on - strand, within GFF2722 at 245.236 kb on - strand, within GFF2722 at 245.314 kb on + strand, within GFF2722 at 245.314 kb on + strand, within GFF2722 at 245.315 kb on - strand, within GFF2722 at 245.531 kb on - strand, within GFF2722 at 245.562 kb on + strand, within GFF2722 at 245.563 kb on - strand, within GFF2722 at 245.563 kb on - strand, within GFF2722 at 245.807 kb on + strand at 245.831 kb on + strand at 245.831 kb on + strand at 245.885 kb on - strand at 245.928 kb on - strand, within GFF2723 at 246.010 kb on - strand, within GFF2723 at 246.199 kb on + strand, within GFF2723 at 246.541 kb on - strand, within GFF2723 at 246.581 kb on + strand, within GFF2723 at 246.582 kb on - strand, within GFF2723 at 246.594 kb on - strand, within GFF2723 at 246.710 kb on + strand, within GFF2723 at 246.787 kb on - strand, within GFF2723 at 246.976 kb on + strand, within GFF2723 at 247.049 kb on + strand, within GFF2723 at 247.153 kb on + strand, within GFF2723 at 247.324 kb on - strand at 247.856 kb on - strand, within GFF2724 at 247.979 kb on + strand, within GFF2724 at 247.986 kb on + strand, within GFF2724 at 247.987 kb on - strand, within GFF2724 at 247.987 kb on - strand, within GFF2724 at 247.987 kb on - strand, within GFF2724 at 247.987 kb on - strand, within GFF2724 at 248.064 kb on - strand, within GFF2724 at 248.158 kb on + strand, within GFF2724 at 248.158 kb on + strand, within GFF2724 at 248.158 kb on + strand, within GFF2724 at 248.160 kb on + strand, within GFF2724
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 244,812 + GFF2722 0.41 +0.4 244,823 + GFF2722 0.42 -0.7 244,914 - GFF2722 0.48 -0.2 244,950 - GFF2722 0.50 -0.6 245,009 - GFF2722 0.54 -0.2 245,012 - GFF2722 0.54 -0.5 245,012 - GFF2722 0.54 -0.3 245,012 - GFF2722 0.54 +0.9 245,042 - GFF2722 0.56 +0.9 245,042 - GFF2722 0.56 -0.1 245,043 + GFF2722 0.56 +0.3 245,044 - GFF2722 0.56 -0.4 245,182 - GFF2722 0.65 +0.3 245,236 - GFF2722 0.68 +1.4 245,314 + GFF2722 0.73 +0.3 245,314 + GFF2722 0.73 +0.1 245,315 - GFF2722 0.73 -1.9 245,531 - GFF2722 0.87 +0.9 245,562 + GFF2722 0.89 +0.7 245,563 - GFF2722 0.89 +0.4 245,563 - GFF2722 0.89 -0.8 245,807 + +1.3 245,831 + -0.7 245,831 + +0.1 245,885 - -0.2 245,928 - GFF2723 0.12 +1.1 246,010 - GFF2723 0.18 +0.6 246,199 + GFF2723 0.29 +1.1 246,541 - GFF2723 0.51 +0.7 246,581 + GFF2723 0.53 +0.3 246,582 - GFF2723 0.53 -0.1 246,594 - GFF2723 0.54 +0.4 246,710 + GFF2723 0.61 +0.3 246,787 - GFF2723 0.66 +1.0 246,976 + GFF2723 0.78 +1.9 247,049 + GFF2723 0.83 -0.3 247,153 + GFF2723 0.89 -0.0 247,324 - +0.5 247,856 - GFF2724 0.39 +0.1 247,979 + GFF2724 0.48 +0.9 247,986 + GFF2724 0.48 +0.1 247,987 - GFF2724 0.48 +0.6 247,987 - GFF2724 0.48 +0.7 247,987 - GFF2724 0.48 +0.2 247,987 - GFF2724 0.48 -0.0 248,064 - GFF2724 0.54 -0.6 248,158 + GFF2724 0.61 -1.3 248,158 + GFF2724 0.61 +0.3 248,158 + GFF2724 0.61 -0.9 248,160 + GFF2724 0.61 +0.2
Or see this region's nucleotide sequence