Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1860 and GFF1861 overlap by 4 nucleotides GFF1861 and GFF1862 overlap by 4 nucleotides
GFF1860 - UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305) / UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13), at 52,486 to 54,468
GFF1860
GFF1861 - Polymyxin resistance protein PmrJ, predicted deacetylase, at 54,465 to 55,364
GFF1861
GFF1862 - Polymyxin resistance protein ArnT, undecaprenyl phosphate-alpha-L-Ara4N transferase; Melittin resistance protein PqaB, at 55,361 to 57,007
GFF1862
Position (kb) 54
55
56 Strain fitness (log2 ratio) -1
0
1 at 53.516 kb on - strand, within GFF1860 at 53.589 kb on + strand, within GFF1860 at 53.757 kb on + strand, within GFF1860 at 53.791 kb on + strand, within GFF1860 at 53.792 kb on - strand, within GFF1860 at 54.174 kb on + strand, within GFF1860 at 54.204 kb on + strand, within GFF1860 at 54.243 kb on + strand, within GFF1860 at 54.304 kb on - strand at 54.307 kb on + strand at 54.308 kb on - strand at 54.497 kb on + strand at 54.649 kb on + strand, within GFF1861 at 54.778 kb on + strand, within GFF1861 at 54.969 kb on + strand, within GFF1861 at 54.970 kb on - strand, within GFF1861 at 54.971 kb on + strand, within GFF1861 at 55.054 kb on + strand, within GFF1861 at 55.153 kb on - strand, within GFF1861 at 55.155 kb on - strand, within GFF1861 at 55.155 kb on - strand, within GFF1861 at 55.155 kb on - strand, within GFF1861 at 55.301 kb on - strand at 55.412 kb on + strand at 55.413 kb on - strand at 55.415 kb on + strand at 55.415 kb on + strand at 55.416 kb on - strand at 55.687 kb on + strand, within GFF1862 at 55.739 kb on + strand, within GFF1862 at 55.755 kb on - strand, within GFF1862 at 55.791 kb on - strand, within GFF1862 at 55.893 kb on + strand, within GFF1862 at 55.920 kb on + strand, within GFF1862 at 56.141 kb on + strand, within GFF1862 at 56.300 kb on + strand, within GFF1862 at 56.300 kb on + strand, within GFF1862 at 56.300 kb on + strand, within GFF1862 at 56.300 kb on + strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.362 kb on - strand, within GFF1862
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 53,516 - GFF1860 0.52 -0.5 53,589 + GFF1860 0.56 -0.5 53,757 + GFF1860 0.64 +0.1 53,791 + GFF1860 0.66 -0.4 53,792 - GFF1860 0.66 -0.0 54,174 + GFF1860 0.85 +0.2 54,204 + GFF1860 0.87 -1.0 54,243 + GFF1860 0.89 +0.4 54,304 - +0.6 54,307 + -1.3 54,308 - -0.3 54,497 + +1.0 54,649 + GFF1861 0.20 +0.5 54,778 + GFF1861 0.35 +0.0 54,969 + GFF1861 0.56 +0.8 54,970 - GFF1861 0.56 +0.4 54,971 + GFF1861 0.56 +1.0 55,054 + GFF1861 0.65 +0.6 55,153 - GFF1861 0.76 +0.5 55,155 - GFF1861 0.77 -0.4 55,155 - GFF1861 0.77 -0.4 55,155 - GFF1861 0.77 +0.9 55,301 - +0.0 55,412 + +0.6 55,413 - +0.1 55,415 + -0.2 55,415 + +0.3 55,416 - +0.2 55,687 + GFF1862 0.20 -0.7 55,739 + GFF1862 0.23 +0.5 55,755 - GFF1862 0.24 +0.0 55,791 - GFF1862 0.26 +0.0 55,893 + GFF1862 0.32 -0.0 55,920 + GFF1862 0.34 +1.0 56,141 + GFF1862 0.47 -0.7 56,300 + GFF1862 0.57 +0.2 56,300 + GFF1862 0.57 +0.6 56,300 + GFF1862 0.57 +0.5 56,300 + GFF1862 0.57 -0.5 56,301 - GFF1862 0.57 +1.0 56,301 - GFF1862 0.57 -0.4 56,301 - GFF1862 0.57 +0.4 56,301 - GFF1862 0.57 +0.5 56,301 - GFF1862 0.57 +1.0 56,362 - GFF1862 0.61 +1.0
Or see this region's nucleotide sequence