Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1544 and GFF1545 are separated by 872 nucleotides GFF1545 and GFF1546 are separated by 80 nucleotides
GFF1543 - DNA-invertase, at 3,024 to 3,596
GFF1543
GFF1544 - hypothetical protein, at 3,549 to 3,686
GFF1544
GFF1545 - Glycosyltransferase IroB, at 4,559 to 5,674
GFF1545
GFF1546 - ABC transporter protein IroC, at 5,755 to 9,408
GFF1546
Position (kb) 4
5
6 Strain fitness (log2 ratio) -1
0
1
2 at 3.722 kb on + strand at 3.726 kb on + strand at 3.726 kb on + strand at 3.911 kb on - strand at 4.217 kb on + strand at 4.224 kb on - strand at 4.233 kb on + strand at 4.304 kb on - strand at 4.379 kb on - strand at 4.466 kb on - strand at 4.473 kb on - strand at 4.473 kb on - strand at 4.498 kb on - strand at 4.565 kb on - strand at 4.565 kb on - strand at 4.589 kb on + strand at 4.602 kb on - strand at 4.650 kb on + strand at 4.650 kb on + strand at 4.651 kb on - strand at 4.778 kb on - strand, within GFF1545 at 4.916 kb on - strand, within GFF1545 at 4.919 kb on + strand, within GFF1545 at 5.118 kb on - strand, within GFF1545 at 5.128 kb on + strand, within GFF1545 at 5.164 kb on + strand, within GFF1545 at 5.165 kb on - strand, within GFF1545 at 5.265 kb on + strand, within GFF1545 at 5.313 kb on + strand, within GFF1545 at 5.826 kb on + strand at 5.826 kb on + strand at 5.827 kb on - strand at 5.972 kb on + strand at 6.048 kb on + strand at 6.159 kb on - strand, within GFF1546 at 6.159 kb on - strand, within GFF1546 at 6.205 kb on + strand, within GFF1546 at 6.205 kb on + strand, within GFF1546 at 6.205 kb on + strand, within GFF1546 at 6.205 kb on + strand, within GFF1546 at 6.205 kb on + strand, within GFF1546 at 6.205 kb on + strand, within GFF1546 at 6.205 kb on + strand, within GFF1546 at 6.208 kb on + strand, within GFF1546 at 6.208 kb on + strand, within GFF1546 at 6.233 kb on + strand, within GFF1546 at 6.369 kb on + strand, within GFF1546 at 6.369 kb on + strand, within GFF1546 at 6.651 kb on - strand, within GFF1546
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 3,722 + +0.2 3,726 + -0.1 3,726 + -1.2 3,911 - -0.2 4,217 + -0.1 4,224 - +0.7 4,233 + -0.0 4,304 - -0.2 4,379 - +0.6 4,466 - -0.3 4,473 - -0.5 4,473 - -0.0 4,498 - +0.1 4,565 - +0.0 4,565 - -0.1 4,589 + -0.3 4,602 - +0.1 4,650 + -0.1 4,650 + +0.1 4,651 - -0.3 4,778 - GFF1545 0.20 +0.1 4,916 - GFF1545 0.32 -0.6 4,919 + GFF1545 0.32 +1.4 5,118 - GFF1545 0.50 +0.1 5,128 + GFF1545 0.51 -0.2 5,164 + GFF1545 0.54 +1.2 5,165 - GFF1545 0.54 +0.1 5,265 + GFF1545 0.63 +0.4 5,313 + GFF1545 0.68 +2.2 5,826 + -0.4 5,826 + +0.6 5,827 - -0.1 5,972 + +0.1 6,048 + +0.4 6,159 - GFF1546 0.11 -1.0 6,159 - GFF1546 0.11 -0.0 6,205 + GFF1546 0.12 -0.6 6,205 + GFF1546 0.12 -0.9 6,205 + GFF1546 0.12 +0.4 6,205 + GFF1546 0.12 -0.4 6,205 + GFF1546 0.12 -0.6 6,205 + GFF1546 0.12 +0.2 6,205 + GFF1546 0.12 +0.6 6,208 + GFF1546 0.12 -0.3 6,208 + GFF1546 0.12 -0.0 6,233 + GFF1546 0.13 -1.5 6,369 + GFF1546 0.17 -0.4 6,369 + GFF1546 0.17 +0.1 6,651 - GFF1546 0.25 +0.0
Or see this region's nucleotide sequence