Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5529 and Atu5530 are separated by 12 nucleotides Atu5530 and Atu5531 are separated by 247 nucleotides Atu5531 and Atu5532 are separated by 238 nucleotides
Atu5529: Atu5529 - two component sensor kinase, at 522,963 to 524,114
Atu5529
Atu5530: Atu5530 - two component response regulator, at 524,127 to 524,735
Atu5530
Atu5531: Atu5531 - ABC transporter substrate binding protein, at 524,983 to 526,233
Atu5531
Atu5532: Atu5532 - ABC transporter membrane spanning protein, at 526,472 to 527,281
Atu5532
Position (kb)
524
525
526
527 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 523.998 kb on - strand, within Atu5529 at 523.998 kb on - strand, within Atu5529 at 524.098 kb on + strand at 524.103 kb on + strand at 524.116 kb on - strand at 524.116 kb on - strand at 524.129 kb on - strand at 524.141 kb on + strand at 524.165 kb on + strand at 524.188 kb on - strand, within Atu5530 at 524.254 kb on - strand, within Atu5530 at 524.254 kb on - strand, within Atu5530 at 524.254 kb on - strand, within Atu5530 at 524.254 kb on - strand, within Atu5530 at 524.276 kb on + strand, within Atu5530 at 524.278 kb on + strand, within Atu5530 at 524.348 kb on + strand, within Atu5530 at 524.348 kb on + strand, within Atu5530 at 524.348 kb on + strand, within Atu5530 at 524.393 kb on + strand, within Atu5530 at 524.400 kb on + strand, within Atu5530 at 524.401 kb on - strand, within Atu5530 at 524.401 kb on - strand, within Atu5530 at 524.412 kb on + strand, within Atu5530 at 524.412 kb on + strand, within Atu5530 at 524.412 kb on + strand, within Atu5530 at 524.412 kb on + strand, within Atu5530 at 524.440 kb on - strand, within Atu5530 at 524.551 kb on - strand, within Atu5530 at 524.634 kb on - strand, within Atu5530 at 524.665 kb on - strand, within Atu5530 at 524.853 kb on + strand at 524.886 kb on + strand at 524.930 kb on + strand at 524.930 kb on + strand at 524.930 kb on + strand at 524.930 kb on + strand at 524.938 kb on - strand at 524.938 kb on - strand at 524.945 kb on + strand at 524.970 kb on - strand at 524.988 kb on + strand at 524.994 kb on - strand at 525.136 kb on + strand, within Atu5531 at 525.144 kb on - strand, within Atu5531 at 525.212 kb on - strand, within Atu5531 at 525.231 kb on - strand, within Atu5531 at 525.246 kb on - strand, within Atu5531 at 525.305 kb on + strand, within Atu5531 at 525.341 kb on + strand, within Atu5531 at 525.351 kb on + strand, within Atu5531 at 525.356 kb on + strand, within Atu5531 at 525.356 kb on + strand, within Atu5531 at 525.361 kb on + strand, within Atu5531 at 525.364 kb on - strand, within Atu5531 at 525.366 kb on + strand, within Atu5531 at 525.396 kb on - strand, within Atu5531 at 525.436 kb on - strand, within Atu5531 at 525.560 kb on - strand, within Atu5531 at 525.744 kb on - strand, within Atu5531 at 525.824 kb on + strand, within Atu5531 at 525.824 kb on + strand, within Atu5531 at 525.824 kb on + strand, within Atu5531 at 525.870 kb on + strand, within Atu5531 at 525.870 kb on + strand, within Atu5531 at 525.870 kb on + strand, within Atu5531 at 525.878 kb on - strand, within Atu5531 at 525.878 kb on - strand, within Atu5531 at 525.880 kb on + strand, within Atu5531 at 526.022 kb on - strand, within Atu5531 at 526.022 kb on - strand, within Atu5531 at 526.038 kb on + strand, within Atu5531 at 526.038 kb on + strand, within Atu5531 at 526.046 kb on - strand, within Atu5531 at 526.078 kb on - strand, within Atu5531 at 526.116 kb on + strand at 526.196 kb on - strand at 526.227 kb on + strand at 526.229 kb on - strand at 526.229 kb on - strand at 526.233 kb on + strand at 526.285 kb on - strand at 526.402 kb on + strand at 526.416 kb on + strand at 526.422 kb on - strand at 526.493 kb on - strand at 526.531 kb on + strand at 526.569 kb on + strand, within Atu5532 at 526.670 kb on + strand, within Atu5532 at 526.707 kb on + strand, within Atu5532 at 526.709 kb on - strand, within Atu5532 at 526.713 kb on + strand, within Atu5532 at 526.713 kb on + strand, within Atu5532 at 526.715 kb on - strand, within Atu5532 at 526.782 kb on + strand, within Atu5532 at 526.830 kb on - strand, within Atu5532 at 526.916 kb on - strand, within Atu5532 at 526.925 kb on + strand, within Atu5532 at 526.974 kb on + strand, within Atu5532 at 527.017 kb on - strand, within Atu5532 at 527.142 kb on - strand, within Atu5532 at 527.143 kb on - strand, within Atu5532
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 523,998 - Atu5529 0.90 +0.2 523,998 - Atu5529 0.90 +4.1 524,098 + +1.0 524,103 + -0.5 524,116 - +1.3 524,116 - +1.2 524,129 - +0.1 524,141 + +0.4 524,165 + +0.2 524,188 - Atu5530 0.10 +1.0 524,254 - Atu5530 0.21 -0.9 524,254 - Atu5530 0.21 -0.6 524,254 - Atu5530 0.21 -0.3 524,254 - Atu5530 0.21 +1.0 524,276 + Atu5530 0.24 +0.3 524,278 + Atu5530 0.25 +2.5 524,348 + Atu5530 0.36 +0.0 524,348 + Atu5530 0.36 -0.7 524,348 + Atu5530 0.36 +0.4 524,393 + Atu5530 0.44 +0.7 524,400 + Atu5530 0.45 +0.5 524,401 - Atu5530 0.45 -0.1 524,401 - Atu5530 0.45 +0.3 524,412 + Atu5530 0.47 -0.3 524,412 + Atu5530 0.47 +0.1 524,412 + Atu5530 0.47 +1.1 524,412 + Atu5530 0.47 -0.1 524,440 - Atu5530 0.51 +0.2 524,551 - Atu5530 0.70 +1.0 524,634 - Atu5530 0.83 +1.8 524,665 - Atu5530 0.88 +1.1 524,853 + +0.1 524,886 + -0.8 524,930 + +0.3 524,930 + -0.1 524,930 + +0.3 524,930 + -1.1 524,938 - +0.3 524,938 - -0.7 524,945 + +1.1 524,970 - -0.1 524,988 + -0.4 524,994 - -1.2 525,136 + Atu5531 0.12 -0.4 525,144 - Atu5531 0.13 -1.3 525,212 - Atu5531 0.18 +0.3 525,231 - Atu5531 0.20 +0.7 525,246 - Atu5531 0.21 +0.0 525,305 + Atu5531 0.26 -0.9 525,341 + Atu5531 0.29 +0.7 525,351 + Atu5531 0.29 +1.3 525,356 + Atu5531 0.30 +1.1 525,356 + Atu5531 0.30 +0.9 525,361 + Atu5531 0.30 +0.3 525,364 - Atu5531 0.30 +0.2 525,366 + Atu5531 0.31 -1.4 525,396 - Atu5531 0.33 -1.4 525,436 - Atu5531 0.36 +0.1 525,560 - Atu5531 0.46 +0.9 525,744 - Atu5531 0.61 -0.9 525,824 + Atu5531 0.67 +0.1 525,824 + Atu5531 0.67 +0.3 525,824 + Atu5531 0.67 +0.5 525,870 + Atu5531 0.71 -1.8 525,870 + Atu5531 0.71 -0.8 525,870 + Atu5531 0.71 +2.5 525,878 - Atu5531 0.72 +0.7 525,878 - Atu5531 0.72 -0.4 525,880 + Atu5531 0.72 +2.9 526,022 - Atu5531 0.83 -0.4 526,022 - Atu5531 0.83 +1.5 526,038 + Atu5531 0.84 -0.0 526,038 + Atu5531 0.84 -0.3 526,046 - Atu5531 0.85 +2.1 526,078 - Atu5531 0.88 -0.8 526,116 + +1.4 526,196 - +0.4 526,227 + +2.1 526,229 - +1.1 526,229 - +0.0 526,233 + +0.7 526,285 - -0.3 526,402 + +1.8 526,416 + +0.1 526,422 - +0.7 526,493 - -0.7 526,531 + +0.4 526,569 + Atu5532 0.12 -0.5 526,670 + Atu5532 0.24 +0.9 526,707 + Atu5532 0.29 -1.0 526,709 - Atu5532 0.29 +0.4 526,713 + Atu5532 0.30 +0.8 526,713 + Atu5532 0.30 -0.7 526,715 - Atu5532 0.30 -0.3 526,782 + Atu5532 0.38 -1.3 526,830 - Atu5532 0.44 -0.6 526,916 - Atu5532 0.55 +1.5 526,925 + Atu5532 0.56 +3.4 526,974 + Atu5532 0.62 +0.4 527,017 - Atu5532 0.67 +0.5 527,142 - Atu5532 0.83 -1.4 527,143 - Atu5532 0.83 +0.5
Or see this region's nucleotide sequence