Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_2022

Experiment: L-Leucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_2020 and PfGW456L13_2021 are separated by 197 nucleotidesPfGW456L13_2021 and PfGW456L13_2022 are separated by 100 nucleotidesPfGW456L13_2022 and PfGW456L13_2023 are separated by 1 nucleotidesPfGW456L13_2023 and PfGW456L13_2024 overlap by 4 nucleotides PfGW456L13_2020 - tRNA(Cytosine32)-2-thiocytidine synthetase, at 2,223,929 to 2,224,753 _2020 PfGW456L13_2021 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 2,224,951 to 2,225,625 _2021 PfGW456L13_2022 - FIG00956341: hypothetical protein, at 2,225,726 to 2,226,151 _2022 PfGW456L13_2023 - Flavoprotein WrbA, at 2,226,153 to 2,226,749 _2023 PfGW456L13_2024 - Arsenate reductase (EC 1.20.4.1), at 2,226,746 to 2,227,099 _2024 Position (kb) 2225 2226 2227Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2224.761 kb on + strandat 2224.761 kb on + strandat 2224.761 kb on + strandat 2224.761 kb on + strandat 2224.761 kb on + strandat 2224.762 kb on - strandat 2224.762 kb on - strandat 2224.762 kb on - strandat 2224.762 kb on - strandat 2224.762 kb on - strandat 2224.762 kb on - strandat 2224.762 kb on - strandat 2224.762 kb on - strandat 2224.762 kb on - strandat 2224.826 kb on + strandat 2224.826 kb on + strandat 2224.826 kb on + strandat 2224.826 kb on + strandat 2224.827 kb on - strandat 2224.915 kb on + strandat 2224.915 kb on + strandat 2224.915 kb on + strandat 2224.915 kb on + strandat 2224.915 kb on + strandat 2224.916 kb on - strandat 2224.916 kb on - strandat 2224.916 kb on - strandat 2224.923 kb on + strandat 2224.923 kb on + strandat 2224.923 kb on + strandat 2224.924 kb on - strandat 2224.924 kb on - strandat 2224.924 kb on - strandat 2224.924 kb on - strandat 2224.924 kb on - strandat 2224.960 kb on + strandat 2224.960 kb on + strandat 2224.960 kb on + strandat 2224.960 kb on + strandat 2224.960 kb on + strandat 2224.961 kb on - strandat 2224.961 kb on - strandat 2224.979 kb on - strandat 2224.979 kb on - strandat 2225.075 kb on - strandat 2225.075 kb on - strand, within PfGW456L13_2021at 2225.289 kb on - strand, within PfGW456L13_2021at 2225.289 kb on - strand, within PfGW456L13_2021at 2225.450 kb on + strand, within PfGW456L13_2021at 2225.458 kb on + strand, within PfGW456L13_2021at 2225.458 kb on + strand, within PfGW456L13_2021at 2225.458 kb on + strand, within PfGW456L13_2021at 2225.459 kb on - strand, within PfGW456L13_2021at 2225.459 kb on - strand, within PfGW456L13_2021at 2225.459 kb on - strand, within PfGW456L13_2021at 2225.459 kb on - strand, within PfGW456L13_2021at 2225.465 kb on + strand, within PfGW456L13_2021at 2225.465 kb on + strand, within PfGW456L13_2021at 2225.465 kb on + strand, within PfGW456L13_2021at 2225.466 kb on - strand, within PfGW456L13_2021at 2225.466 kb on - strand, within PfGW456L13_2021at 2225.466 kb on - strand, within PfGW456L13_2021at 2225.608 kb on + strandat 2225.608 kb on + strandat 2225.609 kb on - strandat 2225.609 kb on - strandat 2225.716 kb on + strandat 2225.717 kb on - strandat 2225.790 kb on + strand, within PfGW456L13_2022at 2225.790 kb on + strand, within PfGW456L13_2022at 2225.790 kb on + strand, within PfGW456L13_2022at 2225.790 kb on + strand, within PfGW456L13_2022at 2225.791 kb on - strand, within PfGW456L13_2022at 2225.791 kb on - strand, within PfGW456L13_2022at 2225.791 kb on - strand, within PfGW456L13_2022at 2225.791 kb on - strand, within PfGW456L13_2022at 2225.791 kb on - strand, within PfGW456L13_2022at 2225.791 kb on - strand, within PfGW456L13_2022at 2225.791 kb on - strand, within PfGW456L13_2022at 2225.791 kb on - strandat 2225.865 kb on + strand, within PfGW456L13_2022at 2225.866 kb on - strand, within PfGW456L13_2022at 2225.892 kb on + strand, within PfGW456L13_2022at 2225.892 kb on + strand, within PfGW456L13_2022at 2225.892 kb on + strand, within PfGW456L13_2022at 2225.893 kb on - strand, within PfGW456L13_2022at 2226.033 kb on + strand, within PfGW456L13_2022at 2226.033 kb on + strand, within PfGW456L13_2022at 2226.033 kb on + strand, within PfGW456L13_2022at 2226.033 kb on + strand, within PfGW456L13_2022at 2226.033 kb on + strand, within PfGW456L13_2022at 2226.034 kb on - strand, within PfGW456L13_2022at 2226.034 kb on - strand, within PfGW456L13_2022at 2226.036 kb on + strand, within PfGW456L13_2022at 2226.036 kb on + strand, within PfGW456L13_2022at 2226.036 kb on + strand, within PfGW456L13_2022at 2226.317 kb on - strand, within PfGW456L13_2023at 2226.481 kb on + strand, within PfGW456L13_2023at 2226.481 kb on + strand, within PfGW456L13_2023at 2226.481 kb on + strand, within PfGW456L13_2023at 2226.568 kb on + strand, within PfGW456L13_2023at 2226.568 kb on + strandat 2226.568 kb on + strandat 2226.718 kb on + strandat 2226.719 kb on - strandat 2226.719 kb on - strandat 2226.719 kb on - strandat 2226.721 kb on + strandat 2226.721 kb on + strandat 2226.722 kb on - strandat 2226.722 kb on - strandat 2226.736 kb on + strandat 2226.736 kb on + strandat 2226.736 kb on + strandat 2226.737 kb on - strandat 2226.737 kb on - strandat 2226.737 kb on - strandat 2226.737 kb on - strandat 2226.903 kb on + strand, within PfGW456L13_2024at 2226.904 kb on - strand, within PfGW456L13_2024at 2226.904 kb on - strand, within PfGW456L13_2024at 2226.904 kb on - strand, within PfGW456L13_2024at 2226.904 kb on - strand, within PfGW456L13_2024at 2227.080 kb on + strandat 2227.080 kb on + strandat 2227.080 kb on + strandat 2227.081 kb on - strandat 2227.119 kb on + strandat 2227.132 kb on - strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.137 kb on - strandat 2227.137 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (N)
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2,224,761 + -0.1
2,224,761 + -0.2
2,224,761 + -0.7
2,224,761 + +0.1
2,224,761 + +0.1
2,224,762 - -0.0
2,224,762 - +0.9
2,224,762 - -0.7
2,224,762 - +0.2
2,224,762 - +0.4
2,224,762 - -0.8
2,224,762 - -0.9
2,224,762 - -0.8
2,224,762 - +1.6
2,224,826 + +1.8
2,224,826 + +0.1
2,224,826 + +0.1
2,224,826 + -0.8
2,224,827 - -1.2
2,224,915 + -0.6
2,224,915 + -0.9
2,224,915 + -0.7
2,224,915 + +0.0
2,224,915 + +0.8
2,224,916 - +0.6
2,224,916 - -1.9
2,224,916 - -1.5
2,224,923 + -0.1
2,224,923 + -0.0
2,224,923 + +0.9
2,224,924 - -0.9
2,224,924 - -0.4
2,224,924 - -0.2
2,224,924 - +1.2
2,224,924 - -1.4
2,224,960 + -1.0
2,224,960 + +0.7
2,224,960 + +1.1
2,224,960 + +0.2
2,224,960 + +0.2
2,224,961 - -0.3
2,224,961 - +0.1
2,224,979 - -1.1
2,224,979 - +0.3
2,225,075 - +0.9
2,225,075 - PfGW456L13_2021 0.18 +0.7
2,225,289 - PfGW456L13_2021 0.50 +0.3
2,225,289 - PfGW456L13_2021 0.50 +0.2
2,225,450 + PfGW456L13_2021 0.74 +0.1
2,225,458 + PfGW456L13_2021 0.75 +0.3
2,225,458 + PfGW456L13_2021 0.75 +0.4
2,225,458 + PfGW456L13_2021 0.75 -0.1
2,225,459 - PfGW456L13_2021 0.75 -1.2
2,225,459 - PfGW456L13_2021 0.75 -1.8
2,225,459 - PfGW456L13_2021 0.75 +0.9
2,225,459 - PfGW456L13_2021 0.75 +1.9
2,225,465 + PfGW456L13_2021 0.76 -0.8
2,225,465 + PfGW456L13_2021 0.76 +0.1
2,225,465 + PfGW456L13_2021 0.76 +1.0
2,225,466 - PfGW456L13_2021 0.76 -0.5
2,225,466 - PfGW456L13_2021 0.76 -0.2
2,225,466 - PfGW456L13_2021 0.76 -0.1
2,225,608 + +0.3
2,225,608 + -0.7
2,225,609 - +0.3
2,225,609 - +0.7
2,225,716 + -1.1
2,225,717 - -0.2
2,225,790 + PfGW456L13_2022 0.15 +0.0
2,225,790 + PfGW456L13_2022 0.15 +1.1
2,225,790 + PfGW456L13_2022 0.15 -0.5
2,225,790 + PfGW456L13_2022 0.15 -1.1
2,225,791 - PfGW456L13_2022 0.15 +0.3
2,225,791 - PfGW456L13_2022 0.15 -0.6
2,225,791 - PfGW456L13_2022 0.15 -0.8
2,225,791 - PfGW456L13_2022 0.15 +0.3
2,225,791 - PfGW456L13_2022 0.15 +0.3
2,225,791 - PfGW456L13_2022 0.15 -0.4
2,225,791 - PfGW456L13_2022 0.15 -1.0
2,225,791 - +1.3
2,225,865 + PfGW456L13_2022 0.33 -1.1
2,225,866 - PfGW456L13_2022 0.33 -0.9
2,225,892 + PfGW456L13_2022 0.39 -1.8
2,225,892 + PfGW456L13_2022 0.39 +1.0
2,225,892 + PfGW456L13_2022 0.39 -0.9
2,225,893 - PfGW456L13_2022 0.39 -0.8
2,226,033 + PfGW456L13_2022 0.72 +0.1
2,226,033 + PfGW456L13_2022 0.72 -0.5
2,226,033 + PfGW456L13_2022 0.72 +0.7
2,226,033 + PfGW456L13_2022 0.72 -1.2
2,226,033 + PfGW456L13_2022 0.72 +0.2
2,226,034 - PfGW456L13_2022 0.72 +0.5
2,226,034 - PfGW456L13_2022 0.72 +0.2
2,226,036 + PfGW456L13_2022 0.73 +0.4
2,226,036 + PfGW456L13_2022 0.73 -0.8
2,226,036 + PfGW456L13_2022 0.73 +0.3
2,226,317 - PfGW456L13_2023 0.27 -0.9
2,226,481 + PfGW456L13_2023 0.55 -0.5
2,226,481 + PfGW456L13_2023 0.55 -0.2
2,226,481 + PfGW456L13_2023 0.55 +1.1
2,226,568 + PfGW456L13_2023 0.70 +0.4
2,226,568 + -0.4
2,226,568 + -0.4
2,226,718 + -0.4
2,226,719 - -0.8
2,226,719 - +0.0
2,226,719 - +0.4
2,226,721 + -1.1
2,226,721 + +0.5
2,226,722 - +0.3
2,226,722 - +0.4
2,226,736 + +1.4
2,226,736 + -1.4
2,226,736 + +0.3
2,226,737 - +0.7
2,226,737 - -0.8
2,226,737 - -0.3
2,226,737 - +0.7
2,226,903 + PfGW456L13_2024 0.44 -1.1
2,226,904 - PfGW456L13_2024 0.45 -0.2
2,226,904 - PfGW456L13_2024 0.45 -0.5
2,226,904 - PfGW456L13_2024 0.45 +0.5
2,226,904 - PfGW456L13_2024 0.45 -0.5
2,227,080 + +1.0
2,227,080 + -0.4
2,227,080 + -1.3
2,227,081 - +0.3
2,227,119 + -0.1
2,227,132 - -0.4
2,227,136 + +0.0
2,227,136 + -0.2
2,227,136 + -1.2
2,227,136 + +0.5
2,227,136 + -3.3
2,227,136 + +0.9
2,227,137 - +0.9
2,227,137 - +0.1

Or see this region's nucleotide sequence