Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2020 and PfGW456L13_2021 are separated by 197 nucleotides PfGW456L13_2021 and PfGW456L13_2022 are separated by 100 nucleotides PfGW456L13_2022 and PfGW456L13_2023 are separated by 1 nucleotides PfGW456L13_2023 and PfGW456L13_2024 overlap by 4 nucleotides
PfGW456L13_2020 - tRNA(Cytosine32)-2-thiocytidine synthetase, at 2,223,929 to 2,224,753
_2020
PfGW456L13_2021 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 2,224,951 to 2,225,625
_2021
PfGW456L13_2022 - FIG00956341: hypothetical protein, at 2,225,726 to 2,226,151
_2022
PfGW456L13_2023 - Flavoprotein WrbA, at 2,226,153 to 2,226,749
_2023
PfGW456L13_2024 - Arsenate reductase (EC 1.20.4.1), at 2,226,746 to 2,227,099
_2024
Position (kb)
2225
2226
2227 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2224.761 kb on + strand at 2224.761 kb on + strand at 2224.761 kb on + strand at 2224.761 kb on + strand at 2224.761 kb on + strand at 2224.762 kb on - strand at 2224.762 kb on - strand at 2224.762 kb on - strand at 2224.762 kb on - strand at 2224.762 kb on - strand at 2224.762 kb on - strand at 2224.762 kb on - strand at 2224.762 kb on - strand at 2224.762 kb on - strand at 2224.826 kb on + strand at 2224.826 kb on + strand at 2224.826 kb on + strand at 2224.826 kb on + strand at 2224.827 kb on - strand at 2224.915 kb on + strand at 2224.915 kb on + strand at 2224.915 kb on + strand at 2224.915 kb on + strand at 2224.915 kb on + strand at 2224.916 kb on - strand at 2224.916 kb on - strand at 2224.916 kb on - strand at 2224.923 kb on + strand at 2224.923 kb on + strand at 2224.923 kb on + strand at 2224.924 kb on - strand at 2224.924 kb on - strand at 2224.924 kb on - strand at 2224.924 kb on - strand at 2224.924 kb on - strand at 2224.960 kb on + strand at 2224.960 kb on + strand at 2224.960 kb on + strand at 2224.960 kb on + strand at 2224.960 kb on + strand at 2224.961 kb on - strand at 2224.961 kb on - strand at 2224.979 kb on - strand at 2224.979 kb on - strand at 2225.075 kb on - strand at 2225.075 kb on - strand, within PfGW456L13_2021 at 2225.289 kb on - strand, within PfGW456L13_2021 at 2225.289 kb on - strand, within PfGW456L13_2021 at 2225.450 kb on + strand, within PfGW456L13_2021 at 2225.458 kb on + strand, within PfGW456L13_2021 at 2225.458 kb on + strand, within PfGW456L13_2021 at 2225.458 kb on + strand, within PfGW456L13_2021 at 2225.459 kb on - strand, within PfGW456L13_2021 at 2225.459 kb on - strand, within PfGW456L13_2021 at 2225.459 kb on - strand, within PfGW456L13_2021 at 2225.459 kb on - strand, within PfGW456L13_2021 at 2225.465 kb on + strand, within PfGW456L13_2021 at 2225.465 kb on + strand, within PfGW456L13_2021 at 2225.465 kb on + strand, within PfGW456L13_2021 at 2225.466 kb on - strand, within PfGW456L13_2021 at 2225.466 kb on - strand, within PfGW456L13_2021 at 2225.466 kb on - strand, within PfGW456L13_2021 at 2225.608 kb on + strand at 2225.608 kb on + strand at 2225.609 kb on - strand at 2225.609 kb on - strand at 2225.716 kb on + strand at 2225.717 kb on - strand at 2225.790 kb on + strand, within PfGW456L13_2022 at 2225.790 kb on + strand, within PfGW456L13_2022 at 2225.790 kb on + strand, within PfGW456L13_2022 at 2225.790 kb on + strand, within PfGW456L13_2022 at 2225.791 kb on - strand, within PfGW456L13_2022 at 2225.791 kb on - strand, within PfGW456L13_2022 at 2225.791 kb on - strand, within PfGW456L13_2022 at 2225.791 kb on - strand, within PfGW456L13_2022 at 2225.791 kb on - strand, within PfGW456L13_2022 at 2225.791 kb on - strand, within PfGW456L13_2022 at 2225.791 kb on - strand, within PfGW456L13_2022 at 2225.791 kb on - strand at 2225.865 kb on + strand, within PfGW456L13_2022 at 2225.866 kb on - strand, within PfGW456L13_2022 at 2225.892 kb on + strand, within PfGW456L13_2022 at 2225.892 kb on + strand, within PfGW456L13_2022 at 2225.892 kb on + strand, within PfGW456L13_2022 at 2225.893 kb on - strand, within PfGW456L13_2022 at 2226.033 kb on + strand, within PfGW456L13_2022 at 2226.033 kb on + strand, within PfGW456L13_2022 at 2226.033 kb on + strand, within PfGW456L13_2022 at 2226.033 kb on + strand, within PfGW456L13_2022 at 2226.033 kb on + strand, within PfGW456L13_2022 at 2226.034 kb on - strand, within PfGW456L13_2022 at 2226.034 kb on - strand, within PfGW456L13_2022 at 2226.036 kb on + strand, within PfGW456L13_2022 at 2226.036 kb on + strand, within PfGW456L13_2022 at 2226.036 kb on + strand, within PfGW456L13_2022 at 2226.317 kb on - strand, within PfGW456L13_2023 at 2226.481 kb on + strand, within PfGW456L13_2023 at 2226.481 kb on + strand, within PfGW456L13_2023 at 2226.481 kb on + strand, within PfGW456L13_2023 at 2226.568 kb on + strand, within PfGW456L13_2023 at 2226.568 kb on + strand at 2226.568 kb on + strand at 2226.718 kb on + strand at 2226.719 kb on - strand at 2226.719 kb on - strand at 2226.719 kb on - strand at 2226.721 kb on + strand at 2226.721 kb on + strand at 2226.722 kb on - strand at 2226.722 kb on - strand at 2226.736 kb on + strand at 2226.736 kb on + strand at 2226.736 kb on + strand at 2226.737 kb on - strand at 2226.737 kb on - strand at 2226.737 kb on - strand at 2226.737 kb on - strand at 2226.903 kb on + strand, within PfGW456L13_2024 at 2226.904 kb on - strand, within PfGW456L13_2024 at 2226.904 kb on - strand, within PfGW456L13_2024 at 2226.904 kb on - strand, within PfGW456L13_2024 at 2226.904 kb on - strand, within PfGW456L13_2024 at 2227.080 kb on + strand at 2227.080 kb on + strand at 2227.080 kb on + strand at 2227.081 kb on - strand at 2227.119 kb on + strand at 2227.132 kb on - strand at 2227.136 kb on + strand at 2227.136 kb on + strand at 2227.136 kb on + strand at 2227.136 kb on + strand at 2227.136 kb on + strand at 2227.136 kb on + strand at 2227.137 kb on - strand at 2227.137 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,224,761 + -0.1 2,224,761 + -0.2 2,224,761 + -0.7 2,224,761 + +0.1 2,224,761 + +0.1 2,224,762 - -0.0 2,224,762 - +0.9 2,224,762 - -0.7 2,224,762 - +0.2 2,224,762 - +0.4 2,224,762 - -0.8 2,224,762 - -0.9 2,224,762 - -0.8 2,224,762 - +1.6 2,224,826 + +1.8 2,224,826 + +0.1 2,224,826 + +0.1 2,224,826 + -0.8 2,224,827 - -1.2 2,224,915 + -0.6 2,224,915 + -0.9 2,224,915 + -0.7 2,224,915 + +0.0 2,224,915 + +0.8 2,224,916 - +0.6 2,224,916 - -1.9 2,224,916 - -1.5 2,224,923 + -0.1 2,224,923 + -0.0 2,224,923 + +0.9 2,224,924 - -0.9 2,224,924 - -0.4 2,224,924 - -0.2 2,224,924 - +1.2 2,224,924 - -1.4 2,224,960 + -1.0 2,224,960 + +0.7 2,224,960 + +1.1 2,224,960 + +0.2 2,224,960 + +0.2 2,224,961 - -0.3 2,224,961 - +0.1 2,224,979 - -1.1 2,224,979 - +0.3 2,225,075 - +0.9 2,225,075 - PfGW456L13_2021 0.18 +0.7 2,225,289 - PfGW456L13_2021 0.50 +0.3 2,225,289 - PfGW456L13_2021 0.50 +0.2 2,225,450 + PfGW456L13_2021 0.74 +0.1 2,225,458 + PfGW456L13_2021 0.75 +0.3 2,225,458 + PfGW456L13_2021 0.75 +0.4 2,225,458 + PfGW456L13_2021 0.75 -0.1 2,225,459 - PfGW456L13_2021 0.75 -1.2 2,225,459 - PfGW456L13_2021 0.75 -1.8 2,225,459 - PfGW456L13_2021 0.75 +0.9 2,225,459 - PfGW456L13_2021 0.75 +1.9 2,225,465 + PfGW456L13_2021 0.76 -0.8 2,225,465 + PfGW456L13_2021 0.76 +0.1 2,225,465 + PfGW456L13_2021 0.76 +1.0 2,225,466 - PfGW456L13_2021 0.76 -0.5 2,225,466 - PfGW456L13_2021 0.76 -0.2 2,225,466 - PfGW456L13_2021 0.76 -0.1 2,225,608 + +0.3 2,225,608 + -0.7 2,225,609 - +0.3 2,225,609 - +0.7 2,225,716 + -1.1 2,225,717 - -0.2 2,225,790 + PfGW456L13_2022 0.15 +0.0 2,225,790 + PfGW456L13_2022 0.15 +1.1 2,225,790 + PfGW456L13_2022 0.15 -0.5 2,225,790 + PfGW456L13_2022 0.15 -1.1 2,225,791 - PfGW456L13_2022 0.15 +0.3 2,225,791 - PfGW456L13_2022 0.15 -0.6 2,225,791 - PfGW456L13_2022 0.15 -0.8 2,225,791 - PfGW456L13_2022 0.15 +0.3 2,225,791 - PfGW456L13_2022 0.15 +0.3 2,225,791 - PfGW456L13_2022 0.15 -0.4 2,225,791 - PfGW456L13_2022 0.15 -1.0 2,225,791 - +1.3 2,225,865 + PfGW456L13_2022 0.33 -1.1 2,225,866 - PfGW456L13_2022 0.33 -0.9 2,225,892 + PfGW456L13_2022 0.39 -1.8 2,225,892 + PfGW456L13_2022 0.39 +1.0 2,225,892 + PfGW456L13_2022 0.39 -0.9 2,225,893 - PfGW456L13_2022 0.39 -0.8 2,226,033 + PfGW456L13_2022 0.72 +0.1 2,226,033 + PfGW456L13_2022 0.72 -0.5 2,226,033 + PfGW456L13_2022 0.72 +0.7 2,226,033 + PfGW456L13_2022 0.72 -1.2 2,226,033 + PfGW456L13_2022 0.72 +0.2 2,226,034 - PfGW456L13_2022 0.72 +0.5 2,226,034 - PfGW456L13_2022 0.72 +0.2 2,226,036 + PfGW456L13_2022 0.73 +0.4 2,226,036 + PfGW456L13_2022 0.73 -0.8 2,226,036 + PfGW456L13_2022 0.73 +0.3 2,226,317 - PfGW456L13_2023 0.27 -0.9 2,226,481 + PfGW456L13_2023 0.55 -0.5 2,226,481 + PfGW456L13_2023 0.55 -0.2 2,226,481 + PfGW456L13_2023 0.55 +1.1 2,226,568 + PfGW456L13_2023 0.70 +0.4 2,226,568 + -0.4 2,226,568 + -0.4 2,226,718 + -0.4 2,226,719 - -0.8 2,226,719 - +0.0 2,226,719 - +0.4 2,226,721 + -1.1 2,226,721 + +0.5 2,226,722 - +0.3 2,226,722 - +0.4 2,226,736 + +1.4 2,226,736 + -1.4 2,226,736 + +0.3 2,226,737 - +0.7 2,226,737 - -0.8 2,226,737 - -0.3 2,226,737 - +0.7 2,226,903 + PfGW456L13_2024 0.44 -1.1 2,226,904 - PfGW456L13_2024 0.45 -0.2 2,226,904 - PfGW456L13_2024 0.45 -0.5 2,226,904 - PfGW456L13_2024 0.45 +0.5 2,226,904 - PfGW456L13_2024 0.45 -0.5 2,227,080 + +1.0 2,227,080 + -0.4 2,227,080 + -1.3 2,227,081 - +0.3 2,227,119 + -0.1 2,227,132 - -0.4 2,227,136 + +0.0 2,227,136 + -0.2 2,227,136 + -1.2 2,227,136 + +0.5 2,227,136 + -3.3 2,227,136 + +0.9 2,227,137 - +0.9 2,227,137 - +0.1
Or see this region's nucleotide sequence