Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_3425 and PfGW456L13_3426 are separated by 97 nucleotides PfGW456L13_3426 and PfGW456L13_3427 are separated by 33 nucleotides PfGW456L13_3427 and PfGW456L13_3428 overlap by 4 nucleotides
PfGW456L13_3425 - Beta-carotene ketolase (EC 1.14.-.-), at 3,841,785 to 3,843,386
_3425
PfGW456L13_3426 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 3,843,484 to 3,844,305
_3426
PfGW456L13_3427 - Enoyl-CoA hydratase (EC 4.2.1.17), at 3,844,339 to 3,845,157
_3427
PfGW456L13_3428 - Lactoylglutathione lyase and related lyases, at 3,845,154 to 3,846,125
_3428
Position (kb)
3844
3845
3846 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3843.360 kb on - strand at 3843.404 kb on + strand at 3843.405 kb on - strand at 3843.445 kb on + strand at 3843.485 kb on + strand at 3843.485 kb on + strand at 3843.485 kb on + strand at 3843.485 kb on + strand at 3843.485 kb on + strand at 3843.486 kb on - strand at 3843.486 kb on - strand at 3843.486 kb on - strand at 3843.576 kb on + strand, within PfGW456L13_3426 at 3843.577 kb on - strand, within PfGW456L13_3426 at 3843.765 kb on - strand, within PfGW456L13_3426 at 3843.819 kb on - strand, within PfGW456L13_3426 at 3843.834 kb on + strand, within PfGW456L13_3426 at 3843.835 kb on - strand, within PfGW456L13_3426 at 3843.835 kb on - strand, within PfGW456L13_3426 at 3844.270 kb on - strand at 3844.270 kb on - strand at 3844.270 kb on - strand at 3844.270 kb on - strand at 3844.300 kb on - strand at 3844.300 kb on - strand at 3844.300 kb on - strand at 3844.326 kb on - strand at 3844.340 kb on + strand at 3844.341 kb on - strand at 3844.864 kb on + strand, within PfGW456L13_3427 at 3844.865 kb on - strand, within PfGW456L13_3427 at 3845.072 kb on - strand, within PfGW456L13_3427 at 3845.072 kb on - strand, within PfGW456L13_3427 at 3845.151 kb on + strand at 3845.151 kb on + strand at 3845.151 kb on + strand at 3845.151 kb on + strand at 3845.151 kb on + strand at 3845.151 kb on + strand at 3845.152 kb on - strand at 3845.326 kb on + strand, within PfGW456L13_3428 at 3845.326 kb on + strand, within PfGW456L13_3428 at 3845.326 kb on + strand, within PfGW456L13_3428 at 3845.327 kb on - strand, within PfGW456L13_3428 at 3845.327 kb on - strand, within PfGW456L13_3428 at 3845.327 kb on - strand, within PfGW456L13_3428 at 3845.327 kb on - strand, within PfGW456L13_3428 at 3845.327 kb on - strand, within PfGW456L13_3428 at 3845.330 kb on - strand, within PfGW456L13_3428 at 3845.349 kb on - strand, within PfGW456L13_3428 at 3845.356 kb on + strand, within PfGW456L13_3428 at 3845.356 kb on + strand, within PfGW456L13_3428 at 3845.357 kb on - strand, within PfGW456L13_3428 at 3845.357 kb on - strand, within PfGW456L13_3428 at 3845.357 kb on - strand, within PfGW456L13_3428 at 3845.357 kb on - strand, within PfGW456L13_3428 at 3845.380 kb on + strand, within PfGW456L13_3428 at 3845.380 kb on + strand, within PfGW456L13_3428 at 3845.506 kb on - strand, within PfGW456L13_3428 at 3845.506 kb on - strand, within PfGW456L13_3428 at 3845.611 kb on + strand, within PfGW456L13_3428 at 3845.686 kb on + strand, within PfGW456L13_3428 at 3845.706 kb on - strand, within PfGW456L13_3428 at 3845.706 kb on - strand, within PfGW456L13_3428 at 3845.977 kb on - strand, within PfGW456L13_3428 at 3845.998 kb on + strand, within PfGW456L13_3428 at 3845.999 kb on - strand, within PfGW456L13_3428 at 3846.040 kb on + strand at 3846.041 kb on - strand at 3846.041 kb on - strand at 3846.041 kb on - strand at 3846.041 kb on - strand at 3846.125 kb on + strand at 3846.125 kb on + strand at 3846.125 kb on + strand at 3846.125 kb on + strand at 3846.125 kb on + strand at 3846.125 kb on + strand at 3846.125 kb on + strand at 3846.125 kb on + strand at 3846.125 kb on + strand at 3846.126 kb on - strand at 3846.126 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,843,360 - -0.4 3,843,404 + +0.2 3,843,405 - +0.8 3,843,445 + +1.8 3,843,485 + +1.0 3,843,485 + -0.6 3,843,485 + +0.3 3,843,485 + +0.5 3,843,485 + -0.2 3,843,486 - +1.0 3,843,486 - +0.2 3,843,486 - +0.0 3,843,576 + PfGW456L13_3426 0.11 -0.2 3,843,577 - PfGW456L13_3426 0.11 +0.4 3,843,765 - PfGW456L13_3426 0.34 +0.8 3,843,819 - PfGW456L13_3426 0.41 +0.3 3,843,834 + PfGW456L13_3426 0.43 -1.0 3,843,835 - PfGW456L13_3426 0.43 -0.5 3,843,835 - PfGW456L13_3426 0.43 -0.4 3,844,270 - +1.3 3,844,270 - -0.0 3,844,270 - +0.3 3,844,270 - +1.0 3,844,300 - +0.7 3,844,300 - -0.0 3,844,300 - +0.0 3,844,326 - -0.3 3,844,340 + -0.6 3,844,341 - +0.4 3,844,864 + PfGW456L13_3427 0.64 -0.8 3,844,865 - PfGW456L13_3427 0.64 -1.4 3,845,072 - PfGW456L13_3427 0.89 -0.8 3,845,072 - PfGW456L13_3427 0.89 -0.2 3,845,151 + -0.2 3,845,151 + +0.7 3,845,151 + -0.5 3,845,151 + -1.3 3,845,151 + -0.2 3,845,151 + +0.9 3,845,152 - +0.5 3,845,326 + PfGW456L13_3428 0.18 +0.8 3,845,326 + PfGW456L13_3428 0.18 +0.1 3,845,326 + PfGW456L13_3428 0.18 -1.6 3,845,327 - PfGW456L13_3428 0.18 +2.4 3,845,327 - PfGW456L13_3428 0.18 -0.1 3,845,327 - PfGW456L13_3428 0.18 -0.1 3,845,327 - PfGW456L13_3428 0.18 +0.4 3,845,327 - PfGW456L13_3428 0.18 +0.7 3,845,330 - PfGW456L13_3428 0.18 +0.3 3,845,349 - PfGW456L13_3428 0.20 -0.8 3,845,356 + PfGW456L13_3428 0.21 -1.6 3,845,356 + PfGW456L13_3428 0.21 -0.5 3,845,357 - PfGW456L13_3428 0.21 -1.2 3,845,357 - PfGW456L13_3428 0.21 +0.3 3,845,357 - PfGW456L13_3428 0.21 +0.0 3,845,357 - PfGW456L13_3428 0.21 +0.3 3,845,380 + PfGW456L13_3428 0.23 -0.8 3,845,380 + PfGW456L13_3428 0.23 +0.1 3,845,506 - PfGW456L13_3428 0.36 +1.0 3,845,506 - PfGW456L13_3428 0.36 -0.0 3,845,611 + PfGW456L13_3428 0.47 +1.4 3,845,686 + PfGW456L13_3428 0.55 -0.7 3,845,706 - PfGW456L13_3428 0.57 +0.4 3,845,706 - PfGW456L13_3428 0.57 +0.2 3,845,977 - PfGW456L13_3428 0.85 -0.5 3,845,998 + PfGW456L13_3428 0.87 -0.8 3,845,999 - PfGW456L13_3428 0.87 -0.0 3,846,040 + -0.4 3,846,041 - +0.0 3,846,041 - +2.3 3,846,041 - +0.2 3,846,041 - -1.3 3,846,125 + -0.3 3,846,125 + +0.4 3,846,125 + +0.2 3,846,125 + +0.0 3,846,125 + +0.1 3,846,125 + +0.4 3,846,125 + -0.1 3,846,125 + +0.4 3,846,125 + +0.5 3,846,126 - -0.0 3,846,126 - -1.9
Or see this region's nucleotide sequence