Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_3252 and PfGW456L13_3253 are separated by 159 nucleotides PfGW456L13_3253 and PfGW456L13_3254 are separated by 2 nucleotides
PfGW456L13_3252 - Endonuclease/Exonuclease/phosphatase family protein, at 3,647,601 to 3,648,401
_3252
PfGW456L13_3253 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 3,648,561 to 3,650,792
_3253
PfGW456L13_3254 - Trehalose synthase (EC 5.4.99.16), at 3,650,795 to 3,654,136
_3254
Position (kb)
3648
3649
3650
3651 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3647.569 kb on + strand at 3647.569 kb on + strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.570 kb on - strand at 3647.573 kb on + strand at 3647.573 kb on + strand at 3647.573 kb on + strand at 3647.573 kb on + strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.574 kb on - strand at 3647.706 kb on - strand, within PfGW456L13_3252 at 3647.759 kb on + strand, within PfGW456L13_3252 at 3647.760 kb on - strand, within PfGW456L13_3252 at 3647.760 kb on - strand, within PfGW456L13_3252 at 3647.850 kb on + strand, within PfGW456L13_3252 at 3647.851 kb on - strand, within PfGW456L13_3252 at 3647.947 kb on - strand, within PfGW456L13_3252 at 3648.213 kb on + strand, within PfGW456L13_3252 at 3648.213 kb on + strand, within PfGW456L13_3252 at 3648.214 kb on - strand, within PfGW456L13_3252 at 3648.214 kb on - strand, within PfGW456L13_3252 at 3648.214 kb on - strand, within PfGW456L13_3252 at 3648.214 kb on - strand, within PfGW456L13_3252 at 3648.214 kb on - strand, within PfGW456L13_3252 at 3648.300 kb on + strand, within PfGW456L13_3252 at 3648.300 kb on + strand, within PfGW456L13_3252 at 3648.301 kb on - strand, within PfGW456L13_3252 at 3648.334 kb on + strand at 3648.539 kb on + strand at 3648.540 kb on - strand at 3648.540 kb on - strand at 3648.673 kb on + strand at 3648.688 kb on + strand at 3648.689 kb on - strand at 3648.689 kb on - strand at 3648.689 kb on - strand at 3648.751 kb on + strand at 3648.980 kb on - strand, within PfGW456L13_3253 at 3649.201 kb on + strand, within PfGW456L13_3253 at 3649.201 kb on + strand, within PfGW456L13_3253 at 3649.201 kb on + strand, within PfGW456L13_3253 at 3649.201 kb on + strand, within PfGW456L13_3253 at 3649.201 kb on + strand, within PfGW456L13_3253 at 3649.202 kb on - strand, within PfGW456L13_3253 at 3649.202 kb on - strand, within PfGW456L13_3253 at 3649.202 kb on - strand, within PfGW456L13_3253 at 3649.202 kb on - strand at 3649.202 kb on - strand, within PfGW456L13_3253 at 3649.202 kb on - strand, within PfGW456L13_3253 at 3649.202 kb on - strand, within PfGW456L13_3253 at 3649.202 kb on - strand, within PfGW456L13_3253 at 3649.264 kb on + strand, within PfGW456L13_3253 at 3649.264 kb on + strand, within PfGW456L13_3253 at 3649.264 kb on + strand, within PfGW456L13_3253 at 3649.264 kb on + strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.265 kb on - strand, within PfGW456L13_3253 at 3649.303 kb on + strand, within PfGW456L13_3253 at 3649.304 kb on - strand, within PfGW456L13_3253 at 3649.304 kb on - strand, within PfGW456L13_3253 at 3649.341 kb on + strand, within PfGW456L13_3253 at 3649.496 kb on - strand, within PfGW456L13_3253 at 3649.585 kb on + strand, within PfGW456L13_3253 at 3649.585 kb on + strand, within PfGW456L13_3253 at 3649.585 kb on + strand, within PfGW456L13_3253 at 3649.586 kb on - strand, within PfGW456L13_3253 at 3649.586 kb on - strand, within PfGW456L13_3253 at 3649.586 kb on - strand, within PfGW456L13_3253 at 3649.586 kb on - strand, within PfGW456L13_3253 at 3649.586 kb on - strand, within PfGW456L13_3253 at 3649.586 kb on - strand, within PfGW456L13_3253 at 3649.586 kb on - strand, within PfGW456L13_3253 at 3649.586 kb on - strand, within PfGW456L13_3253 at 3649.586 kb on - strand, within PfGW456L13_3253 at 3649.615 kb on + strand, within PfGW456L13_3253 at 3649.615 kb on + strand, within PfGW456L13_3253 at 3649.615 kb on + strand, within PfGW456L13_3253 at 3649.664 kb on - strand, within PfGW456L13_3253 at 3649.670 kb on - strand, within PfGW456L13_3253 at 3649.670 kb on - strand, within PfGW456L13_3253 at 3649.805 kb on - strand at 3649.915 kb on + strand, within PfGW456L13_3253 at 3649.916 kb on - strand, within PfGW456L13_3253 at 3649.951 kb on + strand, within PfGW456L13_3253 at 3649.952 kb on - strand, within PfGW456L13_3253 at 3650.194 kb on + strand, within PfGW456L13_3253 at 3650.194 kb on + strand, within PfGW456L13_3253 at 3650.195 kb on - strand, within PfGW456L13_3253 at 3650.195 kb on - strand, within PfGW456L13_3253 at 3650.200 kb on + strand, within PfGW456L13_3253 at 3650.201 kb on - strand, within PfGW456L13_3253 at 3650.201 kb on - strand, within PfGW456L13_3253 at 3650.431 kb on + strand, within PfGW456L13_3253 at 3650.431 kb on + strand, within PfGW456L13_3253 at 3650.431 kb on + strand, within PfGW456L13_3253 at 3650.431 kb on + strand, within PfGW456L13_3253 at 3650.432 kb on - strand, within PfGW456L13_3253 at 3650.432 kb on - strand, within PfGW456L13_3253 at 3650.432 kb on - strand, within PfGW456L13_3253 at 3650.518 kb on + strand, within PfGW456L13_3253 at 3650.518 kb on + strand, within PfGW456L13_3253 at 3650.518 kb on + strand, within PfGW456L13_3253 at 3650.519 kb on - strand, within PfGW456L13_3253 at 3650.519 kb on - strand, within PfGW456L13_3253 at 3650.639 kb on - strand at 3650.702 kb on + strand at 3650.797 kb on - strand at 3650.825 kb on - strand at 3650.845 kb on - strand at 3650.846 kb on + strand at 3650.846 kb on + strand at 3650.846 kb on + strand at 3650.847 kb on - strand at 3650.847 kb on - strand at 3650.847 kb on - strand at 3650.847 kb on - strand at 3650.895 kb on + strand at 3650.895 kb on + strand at 3650.895 kb on + strand at 3650.896 kb on - strand at 3650.908 kb on - strand at 3650.983 kb on + strand at 3650.983 kb on + strand at 3650.983 kb on + strand at 3650.983 kb on + strand at 3650.984 kb on - strand at 3650.984 kb on - strand at 3650.984 kb on - strand at 3651.037 kb on - strand at 3651.121 kb on - strand at 3651.156 kb on + strand, within PfGW456L13_3254 at 3651.156 kb on + strand at 3651.156 kb on + strand, within PfGW456L13_3254 at 3651.157 kb on - strand, within PfGW456L13_3254 at 3651.157 kb on - strand, within PfGW456L13_3254 at 3651.222 kb on + strand, within PfGW456L13_3254 at 3651.222 kb on + strand at 3651.223 kb on - strand, within PfGW456L13_3254 at 3651.223 kb on - strand, within PfGW456L13_3254 at 3651.317 kb on + strand, within PfGW456L13_3254 at 3651.317 kb on + strand, within PfGW456L13_3254 at 3651.317 kb on + strand, within PfGW456L13_3254 at 3651.317 kb on + strand, within PfGW456L13_3254 at 3651.318 kb on - strand, within PfGW456L13_3254 at 3651.318 kb on - strand, within PfGW456L13_3254 at 3651.318 kb on - strand, within PfGW456L13_3254 at 3651.350 kb on + strand, within PfGW456L13_3254 at 3651.394 kb on + strand, within PfGW456L13_3254 at 3651.394 kb on + strand, within PfGW456L13_3254 at 3651.394 kb on + strand at 3651.394 kb on + strand, within PfGW456L13_3254 at 3651.394 kb on + strand, within PfGW456L13_3254 at 3651.395 kb on - strand, within PfGW456L13_3254 at 3651.395 kb on - strand, within PfGW456L13_3254 at 3651.395 kb on - strand, within PfGW456L13_3254 at 3651.395 kb on - strand, within PfGW456L13_3254 at 3651.395 kb on - strand, within PfGW456L13_3254 at 3651.473 kb on - strand, within PfGW456L13_3254 at 3651.553 kb on + strand, within PfGW456L13_3254 at 3651.553 kb on + strand, within PfGW456L13_3254 at 3651.554 kb on - strand, within PfGW456L13_3254 at 3651.588 kb on + strand, within PfGW456L13_3254 at 3651.588 kb on + strand, within PfGW456L13_3254 at 3651.588 kb on + strand, within PfGW456L13_3254 at 3651.588 kb on + strand, within PfGW456L13_3254 at 3651.588 kb on + strand, within PfGW456L13_3254 at 3651.588 kb on + strand, within PfGW456L13_3254 at 3651.588 kb on + strand, within PfGW456L13_3254 at 3651.589 kb on - strand, within PfGW456L13_3254 at 3651.589 kb on - strand, within PfGW456L13_3254 at 3651.589 kb on - strand, within PfGW456L13_3254 at 3651.589 kb on - strand, within PfGW456L13_3254 at 3651.589 kb on - strand, within PfGW456L13_3254 at 3651.589 kb on - strand, within PfGW456L13_3254 at 3651.716 kb on - strand at 3651.766 kb on + strand, within PfGW456L13_3254 at 3651.766 kb on + strand, within PfGW456L13_3254 at 3651.766 kb on + strand, within PfGW456L13_3254 at 3651.767 kb on - strand, within PfGW456L13_3254 at 3651.792 kb on + strand, within PfGW456L13_3254
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,647,569 + -0.0 3,647,569 + +1.3 3,647,570 - +1.3 3,647,570 - -1.2 3,647,570 - -0.2 3,647,570 - +0.1 3,647,570 - -0.2 3,647,570 - -0.9 3,647,570 - -0.3 3,647,570 - +1.1 3,647,570 - -0.1 3,647,570 - -0.0 3,647,570 - +0.1 3,647,573 + +0.1 3,647,573 + +1.9 3,647,573 + -1.2 3,647,573 + +3.7 3,647,574 - +0.1 3,647,574 - +0.4 3,647,574 - -0.8 3,647,574 - -0.1 3,647,574 - +0.2 3,647,574 - +0.1 3,647,574 - +0.6 3,647,574 - -0.8 3,647,574 - -1.0 3,647,574 - +0.7 3,647,574 - -1.6 3,647,574 - +0.6 3,647,574 - +0.2 3,647,706 - PfGW456L13_3252 0.13 +0.1 3,647,759 + PfGW456L13_3252 0.20 +0.7 3,647,760 - PfGW456L13_3252 0.20 -0.3 3,647,760 - PfGW456L13_3252 0.20 -0.8 3,647,850 + PfGW456L13_3252 0.31 -0.3 3,647,851 - PfGW456L13_3252 0.31 -0.5 3,647,947 - PfGW456L13_3252 0.43 -1.7 3,648,213 + PfGW456L13_3252 0.76 +0.9 3,648,213 + PfGW456L13_3252 0.76 +0.4 3,648,214 - PfGW456L13_3252 0.77 -0.1 3,648,214 - PfGW456L13_3252 0.77 -1.9 3,648,214 - PfGW456L13_3252 0.77 -0.6 3,648,214 - PfGW456L13_3252 0.77 -0.3 3,648,214 - PfGW456L13_3252 0.77 -0.1 3,648,300 + PfGW456L13_3252 0.87 +0.1 3,648,300 + PfGW456L13_3252 0.87 -0.0 3,648,301 - PfGW456L13_3252 0.87 -0.4 3,648,334 + +0.7 3,648,539 + +0.9 3,648,540 - -1.9 3,648,540 - +0.9 3,648,673 + -1.7 3,648,688 + -3.1 3,648,689 - -1.9 3,648,689 - -4.0 3,648,689 - -3.5 3,648,751 + -2.1 3,648,980 - PfGW456L13_3253 0.19 -3.0 3,649,201 + PfGW456L13_3253 0.29 -2.9 3,649,201 + PfGW456L13_3253 0.29 -2.8 3,649,201 + PfGW456L13_3253 0.29 -2.3 3,649,201 + PfGW456L13_3253 0.29 -2.4 3,649,201 + PfGW456L13_3253 0.29 -3.4 3,649,202 - PfGW456L13_3253 0.29 -3.5 3,649,202 - PfGW456L13_3253 0.29 -3.4 3,649,202 - PfGW456L13_3253 0.29 -3.4 3,649,202 - -1.1 3,649,202 - PfGW456L13_3253 0.29 -1.8 3,649,202 - PfGW456L13_3253 0.29 -4.4 3,649,202 - PfGW456L13_3253 0.29 -3.3 3,649,202 - PfGW456L13_3253 0.29 -2.6 3,649,264 + PfGW456L13_3253 0.31 +0.2 3,649,264 + PfGW456L13_3253 0.31 -2.5 3,649,264 + PfGW456L13_3253 0.31 -3.3 3,649,264 + PfGW456L13_3253 0.31 -3.3 3,649,265 - PfGW456L13_3253 0.32 -1.6 3,649,265 - PfGW456L13_3253 0.32 -4.6 3,649,265 - PfGW456L13_3253 0.32 -3.1 3,649,265 - PfGW456L13_3253 0.32 -2.7 3,649,265 - PfGW456L13_3253 0.32 -1.0 3,649,265 - PfGW456L13_3253 0.32 -3.9 3,649,265 - PfGW456L13_3253 0.32 -1.9 3,649,265 - PfGW456L13_3253 0.32 -3.7 3,649,265 - PfGW456L13_3253 0.32 -1.9 3,649,265 - PfGW456L13_3253 0.32 -2.9 3,649,265 - PfGW456L13_3253 0.32 -2.2 3,649,303 + PfGW456L13_3253 0.33 -4.1 3,649,304 - PfGW456L13_3253 0.33 -1.7 3,649,304 - PfGW456L13_3253 0.33 -2.8 3,649,341 + PfGW456L13_3253 0.35 -2.6 3,649,496 - PfGW456L13_3253 0.42 -3.8 3,649,585 + PfGW456L13_3253 0.46 -3.8 3,649,585 + PfGW456L13_3253 0.46 -2.8 3,649,585 + PfGW456L13_3253 0.46 -4.1 3,649,586 - PfGW456L13_3253 0.46 -4.2 3,649,586 - PfGW456L13_3253 0.46 -2.2 3,649,586 - PfGW456L13_3253 0.46 -1.7 3,649,586 - PfGW456L13_3253 0.46 -1.0 3,649,586 - PfGW456L13_3253 0.46 -2.9 3,649,586 - PfGW456L13_3253 0.46 -3.0 3,649,586 - PfGW456L13_3253 0.46 -2.1 3,649,586 - PfGW456L13_3253 0.46 -3.4 3,649,586 - PfGW456L13_3253 0.46 -2.6 3,649,615 + PfGW456L13_3253 0.47 -3.6 3,649,615 + PfGW456L13_3253 0.47 -2.2 3,649,615 + PfGW456L13_3253 0.47 -1.7 3,649,664 - PfGW456L13_3253 0.49 -3.6 3,649,670 - PfGW456L13_3253 0.50 -2.4 3,649,670 - PfGW456L13_3253 0.50 -2.1 3,649,805 - -1.4 3,649,915 + PfGW456L13_3253 0.61 -0.8 3,649,916 - PfGW456L13_3253 0.61 -3.1 3,649,951 + PfGW456L13_3253 0.62 -2.8 3,649,952 - PfGW456L13_3253 0.62 -3.1 3,650,194 + PfGW456L13_3253 0.73 -3.1 3,650,194 + PfGW456L13_3253 0.73 -1.3 3,650,195 - PfGW456L13_3253 0.73 -1.6 3,650,195 - PfGW456L13_3253 0.73 -3.5 3,650,200 + PfGW456L13_3253 0.73 -1.9 3,650,201 - PfGW456L13_3253 0.73 -1.3 3,650,201 - PfGW456L13_3253 0.73 -1.4 3,650,431 + PfGW456L13_3253 0.84 +0.4 3,650,431 + PfGW456L13_3253 0.84 -2.6 3,650,431 + PfGW456L13_3253 0.84 -1.7 3,650,431 + PfGW456L13_3253 0.84 -1.2 3,650,432 - PfGW456L13_3253 0.84 -3.6 3,650,432 - PfGW456L13_3253 0.84 -4.4 3,650,432 - PfGW456L13_3253 0.84 -3.2 3,650,518 + PfGW456L13_3253 0.88 -2.1 3,650,518 + PfGW456L13_3253 0.88 -2.7 3,650,518 + PfGW456L13_3253 0.88 -2.4 3,650,519 - PfGW456L13_3253 0.88 -1.9 3,650,519 - PfGW456L13_3253 0.88 -2.5 3,650,639 - -3.8 3,650,702 + -1.1 3,650,797 - -0.8 3,650,825 - +0.0 3,650,845 - +1.2 3,650,846 + -3.0 3,650,846 + -2.6 3,650,846 + -3.6 3,650,847 - +0.7 3,650,847 - -2.1 3,650,847 - -0.4 3,650,847 - +0.2 3,650,895 + -2.1 3,650,895 + +0.1 3,650,895 + -2.2 3,650,896 - +0.3 3,650,908 - +1.2 3,650,983 + +0.7 3,650,983 + -0.0 3,650,983 + +0.0 3,650,983 + -1.9 3,650,984 - -0.1 3,650,984 - +1.1 3,650,984 - +0.9 3,651,037 - -0.3 3,651,121 - -0.0 3,651,156 + PfGW456L13_3254 0.11 -1.7 3,651,156 + +0.9 3,651,156 + PfGW456L13_3254 0.11 -1.1 3,651,157 - PfGW456L13_3254 0.11 +0.3 3,651,157 - PfGW456L13_3254 0.11 +1.3 3,651,222 + PfGW456L13_3254 0.13 +1.7 3,651,222 + -1.1 3,651,223 - PfGW456L13_3254 0.13 -0.4 3,651,223 - PfGW456L13_3254 0.13 -0.3 3,651,317 + PfGW456L13_3254 0.16 +1.1 3,651,317 + PfGW456L13_3254 0.16 +0.6 3,651,317 + PfGW456L13_3254 0.16 +0.1 3,651,317 + PfGW456L13_3254 0.16 +1.4 3,651,318 - PfGW456L13_3254 0.16 +0.1 3,651,318 - PfGW456L13_3254 0.16 -0.5 3,651,318 - PfGW456L13_3254 0.16 +0.7 3,651,350 + PfGW456L13_3254 0.17 -0.9 3,651,394 + PfGW456L13_3254 0.18 -0.9 3,651,394 + PfGW456L13_3254 0.18 +0.5 3,651,394 + +1.5 3,651,394 + PfGW456L13_3254 0.18 -1.1 3,651,394 + PfGW456L13_3254 0.18 +0.5 3,651,395 - PfGW456L13_3254 0.18 +1.3 3,651,395 - PfGW456L13_3254 0.18 +0.2 3,651,395 - PfGW456L13_3254 0.18 +0.9 3,651,395 - PfGW456L13_3254 0.18 +0.0 3,651,395 - PfGW456L13_3254 0.18 -0.3 3,651,473 - PfGW456L13_3254 0.20 +0.3 3,651,553 + PfGW456L13_3254 0.23 -0.7 3,651,553 + PfGW456L13_3254 0.23 +1.6 3,651,554 - PfGW456L13_3254 0.23 -0.1 3,651,588 + PfGW456L13_3254 0.24 -1.0 3,651,588 + PfGW456L13_3254 0.24 -2.4 3,651,588 + PfGW456L13_3254 0.24 +0.6 3,651,588 + PfGW456L13_3254 0.24 -1.0 3,651,588 + PfGW456L13_3254 0.24 -0.9 3,651,588 + PfGW456L13_3254 0.24 +0.8 3,651,588 + PfGW456L13_3254 0.24 -0.2 3,651,589 - PfGW456L13_3254 0.24 +1.4 3,651,589 - PfGW456L13_3254 0.24 +1.2 3,651,589 - PfGW456L13_3254 0.24 -1.8 3,651,589 - PfGW456L13_3254 0.24 +0.8 3,651,589 - PfGW456L13_3254 0.24 +0.3 3,651,589 - PfGW456L13_3254 0.24 +0.4 3,651,716 - +1.9 3,651,766 + PfGW456L13_3254 0.29 +0.9 3,651,766 + PfGW456L13_3254 0.29 +0.4 3,651,766 + PfGW456L13_3254 0.29 +0.6 3,651,767 - PfGW456L13_3254 0.29 +0.5 3,651,792 + PfGW456L13_3254 0.30 -0.5
Or see this region's nucleotide sequence