Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2982 and PfGW456L13_2983 are separated by 140 nucleotides PfGW456L13_2983 and PfGW456L13_2984 are separated by 13 nucleotides PfGW456L13_2984 and PfGW456L13_2985 are separated by 184 nucleotides
PfGW456L13_2982 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (from data) , at 3,325,752 to 3,326,945
_2982
PfGW456L13_2983 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 3,327,086 to 3,328,213
_2983
PfGW456L13_2984 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 3,328,227 to 3,329,000
_2984
PfGW456L13_2985 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 3,329,185 to 3,330,336
_2985
Position (kb)
3327
3328
3329 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3326.130 kb on + strand, within PfGW456L13_2982 at 3326.130 kb on + strand, within PfGW456L13_2982 at 3326.130 kb on + strand, within PfGW456L13_2982 at 3326.131 kb on - strand, within PfGW456L13_2982 at 3326.941 kb on - strand at 3327.000 kb on + strand at 3327.000 kb on + strand at 3327.001 kb on - strand at 3327.006 kb on + strand at 3327.006 kb on + strand at 3327.006 kb on + strand at 3327.006 kb on + strand at 3327.007 kb on - strand at 3327.007 kb on - strand at 3327.049 kb on - strand at 3327.049 kb on - strand at 3327.049 kb on - strand at 3327.064 kb on + strand at 3327.065 kb on - strand at 3327.074 kb on + strand at 3327.075 kb on - strand at 3327.075 kb on - strand at 3327.284 kb on + strand, within PfGW456L13_2983 at 3327.380 kb on + strand, within PfGW456L13_2983 at 3327.381 kb on - strand, within PfGW456L13_2983 at 3327.521 kb on + strand, within PfGW456L13_2983 at 3327.521 kb on + strand, within PfGW456L13_2983 at 3327.521 kb on + strand, within PfGW456L13_2983 at 3327.521 kb on + strand, within PfGW456L13_2983 at 3327.521 kb on + strand, within PfGW456L13_2983 at 3327.522 kb on - strand, within PfGW456L13_2983 at 3327.522 kb on - strand, within PfGW456L13_2983 at 3327.522 kb on - strand, within PfGW456L13_2983 at 3327.522 kb on - strand, within PfGW456L13_2983 at 3327.522 kb on - strand, within PfGW456L13_2983 at 3327.522 kb on - strand, within PfGW456L13_2983 at 3327.522 kb on - strand, within PfGW456L13_2983 at 3327.522 kb on - strand, within PfGW456L13_2983 at 3327.522 kb on - strand, within PfGW456L13_2983 at 3327.631 kb on + strand, within PfGW456L13_2983 at 3327.631 kb on + strand, within PfGW456L13_2983 at 3327.631 kb on + strand, within PfGW456L13_2983 at 3327.631 kb on + strand, within PfGW456L13_2983 at 3327.631 kb on + strand, within PfGW456L13_2983 at 3327.632 kb on - strand, within PfGW456L13_2983 at 3327.632 kb on - strand, within PfGW456L13_2983 at 3327.632 kb on - strand, within PfGW456L13_2983 at 3327.633 kb on + strand, within PfGW456L13_2983 at 3327.634 kb on - strand, within PfGW456L13_2983 at 3327.634 kb on - strand, within PfGW456L13_2983 at 3327.684 kb on + strand, within PfGW456L13_2983 at 3327.684 kb on + strand, within PfGW456L13_2983 at 3327.684 kb on + strand, within PfGW456L13_2983 at 3327.685 kb on - strand, within PfGW456L13_2983 at 3327.685 kb on - strand, within PfGW456L13_2983 at 3327.685 kb on - strand, within PfGW456L13_2983 at 3327.869 kb on + strand, within PfGW456L13_2983 at 3327.869 kb on + strand, within PfGW456L13_2983 at 3328.068 kb on + strand, within PfGW456L13_2983 at 3328.068 kb on + strand, within PfGW456L13_2983 at 3328.068 kb on + strand, within PfGW456L13_2983 at 3328.068 kb on + strand, within PfGW456L13_2983 at 3328.068 kb on + strand, within PfGW456L13_2983 at 3328.069 kb on - strand, within PfGW456L13_2983 at 3328.069 kb on - strand, within PfGW456L13_2983 at 3328.069 kb on - strand, within PfGW456L13_2983 at 3328.069 kb on - strand, within PfGW456L13_2983 at 3328.106 kb on + strand at 3328.137 kb on - strand at 3328.147 kb on - strand at 3328.163 kb on + strand at 3328.163 kb on + strand at 3328.164 kb on - strand at 3328.164 kb on - strand at 3328.164 kb on - strand at 3328.164 kb on - strand at 3328.164 kb on - strand at 3328.164 kb on - strand at 3328.209 kb on + strand at 3328.210 kb on - strand at 3328.210 kb on - strand at 3328.233 kb on + strand at 3328.461 kb on + strand, within PfGW456L13_2984 at 3328.461 kb on + strand, within PfGW456L13_2984 at 3328.461 kb on + strand, within PfGW456L13_2984 at 3328.461 kb on + strand, within PfGW456L13_2984 at 3328.462 kb on - strand, within PfGW456L13_2984 at 3329.003 kb on + strand at 3329.003 kb on + strand at 3329.003 kb on + strand at 3329.003 kb on + strand at 3329.003 kb on + strand at 3329.004 kb on - strand at 3329.004 kb on - strand at 3329.061 kb on + strand at 3329.076 kb on + strand at 3329.076 kb on + strand at 3329.170 kb on + strand at 3329.176 kb on + strand at 3329.177 kb on - strand at 3329.177 kb on - strand at 3329.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,326,130 + PfGW456L13_2982 0.32 -2.5 3,326,130 + PfGW456L13_2982 0.32 -1.9 3,326,130 + PfGW456L13_2982 0.32 -2.0 3,326,131 - PfGW456L13_2982 0.32 -2.7 3,326,941 - -0.9 3,327,000 + -0.8 3,327,000 + +0.8 3,327,001 - -1.1 3,327,006 + -1.0 3,327,006 + -1.7 3,327,006 + -0.2 3,327,006 + -0.7 3,327,007 - -0.0 3,327,007 - +0.2 3,327,049 - -1.3 3,327,049 - +0.5 3,327,049 - -1.0 3,327,064 + -0.0 3,327,065 - -2.4 3,327,074 + +1.2 3,327,075 - -1.7 3,327,075 - +1.3 3,327,284 + PfGW456L13_2983 0.18 -2.5 3,327,380 + PfGW456L13_2983 0.26 -1.6 3,327,381 - PfGW456L13_2983 0.26 -0.8 3,327,521 + PfGW456L13_2983 0.39 -3.1 3,327,521 + PfGW456L13_2983 0.39 -1.6 3,327,521 + PfGW456L13_2983 0.39 -1.0 3,327,521 + PfGW456L13_2983 0.39 -2.8 3,327,521 + PfGW456L13_2983 0.39 -0.9 3,327,522 - PfGW456L13_2983 0.39 -1.8 3,327,522 - PfGW456L13_2983 0.39 -0.5 3,327,522 - PfGW456L13_2983 0.39 -0.2 3,327,522 - PfGW456L13_2983 0.39 -1.3 3,327,522 - PfGW456L13_2983 0.39 -1.6 3,327,522 - PfGW456L13_2983 0.39 -0.5 3,327,522 - PfGW456L13_2983 0.39 -0.2 3,327,522 - PfGW456L13_2983 0.39 -2.7 3,327,522 - PfGW456L13_2983 0.39 -1.3 3,327,631 + PfGW456L13_2983 0.48 -2.1 3,327,631 + PfGW456L13_2983 0.48 -1.4 3,327,631 + PfGW456L13_2983 0.48 -2.3 3,327,631 + PfGW456L13_2983 0.48 -1.4 3,327,631 + PfGW456L13_2983 0.48 -0.2 3,327,632 - PfGW456L13_2983 0.48 +0.2 3,327,632 - PfGW456L13_2983 0.48 +0.0 3,327,632 - PfGW456L13_2983 0.48 -1.4 3,327,633 + PfGW456L13_2983 0.48 -2.4 3,327,634 - PfGW456L13_2983 0.49 -0.8 3,327,634 - PfGW456L13_2983 0.49 -1.6 3,327,684 + PfGW456L13_2983 0.53 -1.1 3,327,684 + PfGW456L13_2983 0.53 +0.0 3,327,684 + PfGW456L13_2983 0.53 -1.4 3,327,685 - PfGW456L13_2983 0.53 -1.6 3,327,685 - PfGW456L13_2983 0.53 -3.2 3,327,685 - PfGW456L13_2983 0.53 -0.7 3,327,869 + PfGW456L13_2983 0.69 -0.2 3,327,869 + PfGW456L13_2983 0.69 -1.7 3,328,068 + PfGW456L13_2983 0.87 -1.5 3,328,068 + PfGW456L13_2983 0.87 +1.0 3,328,068 + PfGW456L13_2983 0.87 -1.5 3,328,068 + PfGW456L13_2983 0.87 -1.8 3,328,068 + PfGW456L13_2983 0.87 -1.8 3,328,069 - PfGW456L13_2983 0.87 -1.9 3,328,069 - PfGW456L13_2983 0.87 -2.9 3,328,069 - PfGW456L13_2983 0.87 -0.3 3,328,069 - PfGW456L13_2983 0.87 -0.6 3,328,106 + -1.2 3,328,137 - -2.5 3,328,147 - -1.7 3,328,163 + -2.5 3,328,163 + -1.6 3,328,164 - -3.3 3,328,164 - -3.8 3,328,164 - -3.2 3,328,164 - -1.0 3,328,164 - -0.5 3,328,164 - -1.8 3,328,209 + -2.5 3,328,210 - -1.8 3,328,210 - -2.4 3,328,233 + -4.5 3,328,461 + PfGW456L13_2984 0.30 -2.7 3,328,461 + PfGW456L13_2984 0.30 -1.3 3,328,461 + PfGW456L13_2984 0.30 -3.9 3,328,461 + PfGW456L13_2984 0.30 -2.1 3,328,462 - PfGW456L13_2984 0.30 -1.8 3,329,003 + -0.3 3,329,003 + -1.8 3,329,003 + +0.9 3,329,003 + +0.6 3,329,003 + -1.0 3,329,004 - -0.3 3,329,004 - +2.1 3,329,061 + -1.0 3,329,076 + +1.9 3,329,076 + -0.8 3,329,170 + -0.6 3,329,176 + +0.4 3,329,177 - +0.1 3,329,177 - -0.9 3,329,177 - -0.9
Or see this region's nucleotide sequence