Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1922 and PfGW456L13_1923 are separated by 424 nucleotides PfGW456L13_1923 and PfGW456L13_1924 overlap by 2 nucleotides PfGW456L13_1924 and PfGW456L13_1925 are separated by 195 nucleotides PfGW456L13_1925 and PfGW456L13_1926 are separated by 197 nucleotides PfGW456L13_1926 and PfGW456L13_1927 are separated by 81 nucleotides
PfGW456L13_1922 - Uncharacterized glutathione S-transferase-like protein, at 2,116,533 to 2,117,171
_1922
PfGW456L13_1923 - hypothetical protein, at 2,117,596 to 2,117,736
_1923
PfGW456L13_1924 - hypothetical protein, at 2,117,735 to 2,117,863
_1924
PfGW456L13_1925 - Gluconate utilization system Gnt-I transcriptional repressor, at 2,118,059 to 2,119,108
_1925
PfGW456L13_1926 - Gluconokinase (EC 2.7.1.12), at 2,119,306 to 2,119,839
_1926
PfGW456L13_1927 - Gluconate permease, at 2,119,921 to 2,121,273
_1927
Position (kb)
2118
2119
2120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2117.211 kb on + strand at 2117.211 kb on + strand at 2117.211 kb on + strand at 2117.211 kb on + strand at 2117.211 kb on + strand at 2117.211 kb on + strand at 2117.211 kb on + strand at 2117.212 kb on - strand at 2117.212 kb on - strand at 2117.212 kb on - strand at 2117.212 kb on - strand at 2117.212 kb on - strand at 2117.212 kb on - strand at 2117.212 kb on - strand at 2117.265 kb on + strand at 2117.265 kb on + strand at 2117.265 kb on + strand at 2117.265 kb on + strand at 2117.265 kb on + strand at 2117.265 kb on + strand at 2117.266 kb on - strand at 2117.266 kb on - strand at 2117.266 kb on - strand at 2117.274 kb on - strand at 2117.287 kb on - strand at 2117.336 kb on + strand at 2117.336 kb on + strand at 2117.337 kb on - strand at 2117.340 kb on + strand at 2117.341 kb on - strand at 2117.341 kb on - strand at 2117.341 kb on - strand at 2117.347 kb on - strand at 2117.378 kb on - strand at 2117.389 kb on + strand at 2117.390 kb on - strand at 2117.390 kb on - strand at 2117.390 kb on - strand at 2117.390 kb on - strand at 2117.438 kb on + strand at 2117.438 kb on + strand at 2117.439 kb on - strand at 2117.443 kb on + strand at 2117.443 kb on + strand at 2117.444 kb on - strand at 2117.481 kb on + strand at 2117.514 kb on + strand at 2117.524 kb on + strand at 2117.525 kb on - strand at 2117.525 kb on - strand at 2117.564 kb on - strand at 2117.564 kb on - strand at 2117.564 kb on - strand at 2117.564 kb on - strand at 2117.602 kb on + strand at 2117.603 kb on - strand at 2117.603 kb on - strand at 2117.935 kb on - strand at 2117.953 kb on + strand at 2117.954 kb on - strand at 2117.954 kb on - strand at 2117.954 kb on - strand at 2117.959 kb on + strand at 2117.960 kb on - strand at 2118.060 kb on + strand at 2118.060 kb on + strand at 2118.060 kb on + strand at 2118.060 kb on + strand at 2118.061 kb on - strand at 2118.061 kb on - strand at 2118.061 kb on - strand at 2118.182 kb on - strand, within PfGW456L13_1925 at 2118.182 kb on - strand, within PfGW456L13_1925 at 2118.507 kb on + strand, within PfGW456L13_1925 at 2118.507 kb on + strand, within PfGW456L13_1925 at 2118.508 kb on - strand, within PfGW456L13_1925 at 2118.621 kb on + strand at 2118.621 kb on + strand, within PfGW456L13_1925 at 2118.621 kb on + strand, within PfGW456L13_1925 at 2118.621 kb on + strand, within PfGW456L13_1925 at 2118.621 kb on + strand, within PfGW456L13_1925 at 2118.621 kb on + strand, within PfGW456L13_1925 at 2118.621 kb on + strand, within PfGW456L13_1925 at 2118.622 kb on - strand, within PfGW456L13_1925 at 2118.622 kb on - strand, within PfGW456L13_1925 at 2118.634 kb on + strand, within PfGW456L13_1925 at 2118.634 kb on + strand, within PfGW456L13_1925 at 2118.634 kb on + strand, within PfGW456L13_1925 at 2118.634 kb on + strand, within PfGW456L13_1925 at 2118.635 kb on - strand, within PfGW456L13_1925 at 2118.693 kb on - strand, within PfGW456L13_1925 at 2118.693 kb on - strand, within PfGW456L13_1925 at 2118.693 kb on - strand, within PfGW456L13_1925 at 2118.693 kb on - strand, within PfGW456L13_1925 at 2118.709 kb on - strand, within PfGW456L13_1925 at 2118.717 kb on + strand, within PfGW456L13_1925 at 2118.717 kb on + strand, within PfGW456L13_1925 at 2118.717 kb on + strand, within PfGW456L13_1925 at 2118.717 kb on + strand, within PfGW456L13_1925 at 2118.717 kb on + strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.718 kb on - strand, within PfGW456L13_1925 at 2118.763 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.915 kb on + strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2118.916 kb on - strand, within PfGW456L13_1925 at 2119.064 kb on - strand at 2119.069 kb on + strand at 2119.070 kb on - strand at 2119.070 kb on - strand at 2119.070 kb on - strand at 2119.126 kb on + strand at 2119.199 kb on + strand at 2119.199 kb on + strand at 2119.199 kb on + strand at 2119.199 kb on + strand at 2119.200 kb on - strand at 2119.200 kb on - strand at 2119.216 kb on + strand at 2119.217 kb on - strand at 2119.265 kb on + strand at 2119.265 kb on + strand at 2119.265 kb on + strand at 2119.266 kb on - strand at 2119.303 kb on + strand at 2119.303 kb on + strand at 2119.303 kb on + strand at 2119.304 kb on - strand at 2119.304 kb on - strand at 2119.771 kb on - strand, within PfGW456L13_1926 at 2119.836 kb on - strand at 2119.889 kb on + strand at 2119.890 kb on - strand at 2119.893 kb on + strand at 2119.893 kb on + strand at 2119.893 kb on + strand at 2119.893 kb on + strand at 2119.894 kb on - strand at 2119.894 kb on - strand at 2119.894 kb on - strand at 2119.894 kb on - strand at 2119.984 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,117,211 + +0.3 2,117,211 + -0.6 2,117,211 + -0.3 2,117,211 + -0.9 2,117,211 + +0.4 2,117,211 + -0.6 2,117,211 + -0.4 2,117,212 - +0.6 2,117,212 - +0.8 2,117,212 - +0.7 2,117,212 - +0.4 2,117,212 - -0.4 2,117,212 - -0.6 2,117,212 - -0.7 2,117,265 + -1.3 2,117,265 + -0.1 2,117,265 + +0.3 2,117,265 + -0.1 2,117,265 + +1.8 2,117,265 + -0.3 2,117,266 - -0.2 2,117,266 - -0.9 2,117,266 - -0.1 2,117,274 - +0.3 2,117,287 - -0.2 2,117,336 + +0.6 2,117,336 + +0.4 2,117,337 - -0.5 2,117,340 + -1.2 2,117,341 - -0.7 2,117,341 - -1.1 2,117,341 - -0.1 2,117,347 - -1.5 2,117,378 - +1.3 2,117,389 + -0.3 2,117,390 - -0.4 2,117,390 - +0.6 2,117,390 - +0.4 2,117,390 - +0.8 2,117,438 + +1.0 2,117,438 + -1.2 2,117,439 - -0.1 2,117,443 + -0.7 2,117,443 + -0.3 2,117,444 - +0.9 2,117,481 + +0.4 2,117,514 + +1.4 2,117,524 + -0.5 2,117,525 - +0.2 2,117,525 - +1.3 2,117,564 - +0.2 2,117,564 - -0.8 2,117,564 - +0.1 2,117,564 - +0.3 2,117,602 + -0.4 2,117,603 - +0.6 2,117,603 - -1.2 2,117,935 - +0.7 2,117,953 + +0.6 2,117,954 - +0.5 2,117,954 - +0.2 2,117,954 - -0.6 2,117,959 + +0.2 2,117,960 - -0.2 2,118,060 + -1.8 2,118,060 + +0.1 2,118,060 + +0.8 2,118,060 + +1.2 2,118,061 - -0.5 2,118,061 - -0.9 2,118,061 - +0.4 2,118,182 - PfGW456L13_1925 0.12 +1.4 2,118,182 - PfGW456L13_1925 0.12 +1.4 2,118,507 + PfGW456L13_1925 0.43 -0.5 2,118,507 + PfGW456L13_1925 0.43 +0.7 2,118,508 - PfGW456L13_1925 0.43 +0.0 2,118,621 + +1.8 2,118,621 + PfGW456L13_1925 0.54 -0.6 2,118,621 + PfGW456L13_1925 0.54 -0.2 2,118,621 + PfGW456L13_1925 0.54 +0.3 2,118,621 + PfGW456L13_1925 0.54 +0.1 2,118,621 + PfGW456L13_1925 0.54 -0.8 2,118,621 + PfGW456L13_1925 0.54 +0.2 2,118,622 - PfGW456L13_1925 0.54 +1.3 2,118,622 - PfGW456L13_1925 0.54 +0.4 2,118,634 + PfGW456L13_1925 0.55 -0.2 2,118,634 + PfGW456L13_1925 0.55 +0.9 2,118,634 + PfGW456L13_1925 0.55 +0.5 2,118,634 + PfGW456L13_1925 0.55 +0.3 2,118,635 - PfGW456L13_1925 0.55 +0.6 2,118,693 - PfGW456L13_1925 0.60 +0.1 2,118,693 - PfGW456L13_1925 0.60 +1.1 2,118,693 - PfGW456L13_1925 0.60 +0.2 2,118,693 - PfGW456L13_1925 0.60 -0.8 2,118,709 - PfGW456L13_1925 0.62 +1.7 2,118,717 + PfGW456L13_1925 0.63 +0.5 2,118,717 + PfGW456L13_1925 0.63 +0.3 2,118,717 + PfGW456L13_1925 0.63 +0.3 2,118,717 + PfGW456L13_1925 0.63 +0.6 2,118,717 + PfGW456L13_1925 0.63 -0.4 2,118,718 - PfGW456L13_1925 0.63 +1.2 2,118,718 - PfGW456L13_1925 0.63 -0.2 2,118,718 - PfGW456L13_1925 0.63 +0.2 2,118,718 - PfGW456L13_1925 0.63 +0.7 2,118,718 - PfGW456L13_1925 0.63 +0.8 2,118,718 - PfGW456L13_1925 0.63 +0.1 2,118,718 - PfGW456L13_1925 0.63 +0.9 2,118,718 - PfGW456L13_1925 0.63 +0.3 2,118,718 - PfGW456L13_1925 0.63 +0.6 2,118,718 - PfGW456L13_1925 0.63 +0.4 2,118,718 - PfGW456L13_1925 0.63 +1.1 2,118,763 + PfGW456L13_1925 0.67 -0.3 2,118,915 + PfGW456L13_1925 0.82 +0.5 2,118,915 + PfGW456L13_1925 0.82 +0.7 2,118,915 + PfGW456L13_1925 0.82 +0.1 2,118,915 + PfGW456L13_1925 0.82 +0.8 2,118,915 + PfGW456L13_1925 0.82 +0.3 2,118,915 + PfGW456L13_1925 0.82 +0.4 2,118,915 + PfGW456L13_1925 0.82 +0.2 2,118,915 + PfGW456L13_1925 0.82 +0.6 2,118,915 + PfGW456L13_1925 0.82 -0.4 2,118,915 + PfGW456L13_1925 0.82 -0.3 2,118,915 + PfGW456L13_1925 0.82 +0.7 2,118,915 + PfGW456L13_1925 0.82 +0.7 2,118,915 + PfGW456L13_1925 0.82 +0.6 2,118,915 + PfGW456L13_1925 0.82 +1.3 2,118,916 - PfGW456L13_1925 0.82 -0.4 2,118,916 - PfGW456L13_1925 0.82 -0.1 2,118,916 - PfGW456L13_1925 0.82 +0.9 2,118,916 - PfGW456L13_1925 0.82 -0.6 2,118,916 - PfGW456L13_1925 0.82 +0.4 2,118,916 - PfGW456L13_1925 0.82 +0.9 2,118,916 - PfGW456L13_1925 0.82 +0.2 2,118,916 - PfGW456L13_1925 0.82 +0.5 2,118,916 - PfGW456L13_1925 0.82 +0.6 2,118,916 - PfGW456L13_1925 0.82 -1.4 2,118,916 - PfGW456L13_1925 0.82 +0.7 2,118,916 - PfGW456L13_1925 0.82 +0.7 2,118,916 - PfGW456L13_1925 0.82 +1.2 2,118,916 - PfGW456L13_1925 0.82 +0.2 2,118,916 - PfGW456L13_1925 0.82 -0.2 2,118,916 - PfGW456L13_1925 0.82 +0.3 2,118,916 - PfGW456L13_1925 0.82 +0.4 2,118,916 - PfGW456L13_1925 0.82 -0.0 2,118,916 - PfGW456L13_1925 0.82 -0.1 2,118,916 - PfGW456L13_1925 0.82 +0.6 2,119,064 - +0.3 2,119,069 + +0.5 2,119,070 - +0.8 2,119,070 - -0.3 2,119,070 - -1.3 2,119,126 + -0.4 2,119,199 + +0.8 2,119,199 + +0.9 2,119,199 + +0.5 2,119,199 + -0.7 2,119,200 - -1.3 2,119,200 - +0.6 2,119,216 + -1.8 2,119,217 - -1.7 2,119,265 + -0.9 2,119,265 + -0.4 2,119,265 + +0.1 2,119,266 - -0.6 2,119,303 + -1.7 2,119,303 + -0.9 2,119,303 + +0.8 2,119,304 - -1.6 2,119,304 - +0.2 2,119,771 - PfGW456L13_1926 0.87 -0.6 2,119,836 - -1.0 2,119,889 + +0.6 2,119,890 - -2.8 2,119,893 + +0.5 2,119,893 + +0.8 2,119,893 + +0.5 2,119,893 + -0.1 2,119,894 - -0.8 2,119,894 - -1.6 2,119,894 - -0.2 2,119,894 - -1.4 2,119,984 - -0.3
Or see this region's nucleotide sequence