Experiment: Butanol 0.6 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1546 and PP_5473 are separated by 18 nucleotides PP_5473 and PP_5474 are separated by 530 nucleotides PP_5474 and PP_1548 overlap by 4 nucleotides PP_1548 and PP_5744 are separated by 331 nucleotides PP_5744 and PP_1549 are separated by 64 nucleotides
PP_1546: PP_1546 - conserved protein of unknown function, at 1,747,002 to 1,747,271
_1546
PP_5473: PP_5473 - putative transcriptional regulator, LuxR family, at 1,747,290 to 1,747,508
_5473
PP_5474: PP_5474 - conserved protein of unknown function of phage origin, at 1,748,039 to 1,748,233
_5474
PP_1548: PP_1548 - conserved protein of unknown function, at 1,748,230 to 1,748,667
_1548
PP_5744: PP_5744 - conserved protein of unknown function, phage origin, at 1,748,999 to 1,749,319
_5744
PP_1549: PP_1549 - Transcriptional regulator, Cro/CI family, at 1,749,384 to 1,750,235
_1549
Position (kb)
1748
1749 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1747.300 kb on + strand at 1747.456 kb on + strand, within PP_5473 at 1747.470 kb on + strand, within PP_5473 at 1747.577 kb on + strand at 1747.578 kb on - strand at 1747.578 kb on - strand at 1747.578 kb on - strand at 1747.578 kb on - strand at 1747.721 kb on + strand at 1747.721 kb on + strand at 1747.722 kb on - strand at 1747.771 kb on + strand at 1747.793 kb on + strand at 1747.803 kb on - strand at 1747.825 kb on - strand at 1747.825 kb on - strand at 1747.888 kb on + strand at 1747.889 kb on - strand at 1747.889 kb on - strand at 1747.919 kb on - strand at 1748.001 kb on + strand at 1748.001 kb on + strand at 1748.001 kb on + strand at 1748.002 kb on - strand at 1748.002 kb on - strand at 1748.002 kb on - strand at 1748.002 kb on - strand at 1748.002 kb on - strand at 1748.040 kb on + strand at 1748.041 kb on - strand at 1748.237 kb on + strand at 1748.237 kb on + strand at 1748.237 kb on + strand at 1748.237 kb on + strand at 1748.237 kb on + strand at 1748.238 kb on - strand at 1748.238 kb on - strand at 1748.238 kb on - strand at 1748.344 kb on - strand, within PP_1548 at 1748.565 kb on - strand, within PP_1548 at 1748.565 kb on - strand, within PP_1548 at 1748.782 kb on + strand at 1748.782 kb on + strand at 1748.782 kb on + strand at 1748.782 kb on + strand at 1748.783 kb on - strand at 1748.783 kb on - strand at 1748.855 kb on - strand at 1748.970 kb on - strand at 1749.025 kb on + strand at 1749.292 kb on + strand at 1749.318 kb on - strand at 1749.324 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Butanol 0.6 vol% remove 1,747,300 + -0.2 1,747,456 + PP_5473 0.76 +0.8 1,747,470 + PP_5473 0.82 +1.0 1,747,577 + +0.7 1,747,578 - +0.5 1,747,578 - -0.2 1,747,578 - -0.6 1,747,578 - -0.6 1,747,721 + +0.1 1,747,721 + -0.1 1,747,722 - +1.1 1,747,771 + -1.2 1,747,793 + -0.1 1,747,803 - -0.4 1,747,825 - -1.2 1,747,825 - +0.3 1,747,888 + +0.4 1,747,889 - -1.0 1,747,889 - -0.2 1,747,919 - +0.5 1,748,001 + -1.0 1,748,001 + -0.7 1,748,001 + -0.1 1,748,002 - -1.6 1,748,002 - -0.1 1,748,002 - +1.1 1,748,002 - +1.5 1,748,002 - +0.1 1,748,040 + -1.2 1,748,041 - +0.6 1,748,237 + +0.1 1,748,237 + +1.1 1,748,237 + +0.5 1,748,237 + +0.9 1,748,237 + -0.9 1,748,238 - -0.5 1,748,238 - +0.1 1,748,238 - +0.3 1,748,344 - PP_1548 0.26 +0.1 1,748,565 - PP_1548 0.76 -0.8 1,748,565 - PP_1548 0.76 -2.7 1,748,782 + +0.9 1,748,782 + +2.1 1,748,782 + +0.7 1,748,782 + -0.2 1,748,783 - -0.2 1,748,783 - -0.4 1,748,855 - +0.5 1,748,970 - +0.1 1,749,025 + -0.4 1,749,292 + -1.0 1,749,318 - -1.2 1,749,324 + -3.7
Or see this region's nucleotide sequence