Experiment: Isobutyl alcohol 0.6 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3473 and PP_3474 overlap by 4 nucleotides PP_3474 and PP_3475 overlap by 4 nucleotides PP_3475 and PP_3476 overlap by 29 nucleotides PP_3476 and PP_3477 overlap by 7 nucleotides PP_3477 and PP_3478 overlap by 4 nucleotides
PP_3473: PP_3473 - curli secretion and assembly outer membrane associated component, at 3,939,861 to 3,940,274
_3473
PP_3474: PP_3474 - putative Curli fiber protein CsgE, at 3,940,271 to 3,940,672
_3474
PP_3475: PP_3475 - putative type II secretory pathway, pseudopilin pulG-related signal peptide protein, at 3,940,669 to 3,941,199
_3475
PP_3476: PP_3476 - Type II secretion system protein G, at 3,941,171 to 3,941,557
_3476
PP_3477: PP_3477 - Putative secretion type II protein related to protein G, at 3,941,551 to 3,942,030
_3477
PP_3478: PP_3478 - putative Secretion protein, at 3,942,027 to 3,943,892
_3478
Position (kb)
3941
3942 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3940.195 kb on + strand, within PP_3473 at 3940.195 kb on + strand, within PP_3473 at 3940.195 kb on + strand, within PP_3473 at 3940.195 kb on + strand, within PP_3473 at 3940.196 kb on - strand, within PP_3473 at 3940.196 kb on - strand, within PP_3473 at 3940.196 kb on - strand, within PP_3473 at 3940.274 kb on + strand at 3940.274 kb on + strand at 3940.275 kb on - strand at 3940.275 kb on - strand at 3940.275 kb on - strand at 3940.275 kb on - strand at 3940.275 kb on - strand at 3940.275 kb on - strand at 3940.307 kb on + strand at 3940.390 kb on + strand, within PP_3474 at 3940.391 kb on - strand, within PP_3474 at 3940.391 kb on - strand, within PP_3474 at 3940.479 kb on - strand, within PP_3474 at 3940.531 kb on - strand, within PP_3474 at 3940.531 kb on - strand, within PP_3474 at 3940.531 kb on - strand, within PP_3474 at 3940.531 kb on - strand, within PP_3474 at 3940.536 kb on + strand, within PP_3474 at 3940.758 kb on - strand, within PP_3475 at 3940.835 kb on + strand, within PP_3475 at 3940.907 kb on + strand, within PP_3475 at 3940.954 kb on + strand, within PP_3475 at 3940.980 kb on - strand, within PP_3475 at 3941.007 kb on - strand, within PP_3475 at 3941.007 kb on - strand, within PP_3475 at 3941.009 kb on + strand, within PP_3475 at 3941.009 kb on + strand, within PP_3475 at 3941.009 kb on + strand, within PP_3475 at 3941.031 kb on - strand, within PP_3475 at 3941.150 kb on + strand at 3941.150 kb on + strand at 3941.150 kb on + strand at 3941.151 kb on - strand at 3941.151 kb on - strand at 3941.154 kb on + strand at 3941.172 kb on + strand at 3941.172 kb on + strand at 3941.172 kb on + strand at 3941.173 kb on - strand at 3941.173 kb on - strand at 3941.322 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.377 kb on - strand, within PP_3476 at 3941.380 kb on - strand, within PP_3476 at 3941.458 kb on - strand at 3941.603 kb on + strand, within PP_3477 at 3941.603 kb on + strand, within PP_3477 at 3941.733 kb on - strand, within PP_3477 at 3941.733 kb on - strand, within PP_3477 at 3941.776 kb on + strand, within PP_3477 at 3941.811 kb on + strand, within PP_3477 at 3941.855 kb on + strand, within PP_3477 at 3941.882 kb on + strand, within PP_3477 at 3941.883 kb on - strand, within PP_3477 at 3941.920 kb on + strand, within PP_3477 at 3941.921 kb on - strand, within PP_3477 at 3942.104 kb on + strand at 3942.532 kb on - strand, within PP_3478 at 3942.553 kb on + strand, within PP_3478 at 3942.554 kb on - strand, within PP_3478
Per-strain Table
Position Strand Gene LocusTag Fraction Isobutyl alcohol 0.6 vol% remove 3,940,195 + PP_3473 0.81 -2.6 3,940,195 + PP_3473 0.81 -0.4 3,940,195 + PP_3473 0.81 +1.5 3,940,195 + PP_3473 0.81 -0.3 3,940,196 - PP_3473 0.81 -0.3 3,940,196 - PP_3473 0.81 -1.7 3,940,196 - PP_3473 0.81 -0.3 3,940,274 + -0.0 3,940,274 + -0.2 3,940,275 - -0.3 3,940,275 - -0.6 3,940,275 - +1.1 3,940,275 - +0.7 3,940,275 - -1.6 3,940,275 - -0.4 3,940,307 + -1.4 3,940,390 + PP_3474 0.30 -3.8 3,940,391 - PP_3474 0.30 -1.0 3,940,391 - PP_3474 0.30 +0.7 3,940,479 - PP_3474 0.52 +0.3 3,940,531 - PP_3474 0.65 +0.6 3,940,531 - PP_3474 0.65 -0.5 3,940,531 - PP_3474 0.65 +0.1 3,940,531 - PP_3474 0.65 -0.1 3,940,536 + PP_3474 0.66 +0.7 3,940,758 - PP_3475 0.17 +0.2 3,940,835 + PP_3475 0.31 +0.0 3,940,907 + PP_3475 0.45 +0.6 3,940,954 + PP_3475 0.54 -0.6 3,940,980 - PP_3475 0.59 -0.7 3,941,007 - PP_3475 0.64 +1.4 3,941,007 - PP_3475 0.64 -0.5 3,941,009 + PP_3475 0.64 +0.2 3,941,009 + PP_3475 0.64 -1.0 3,941,009 + PP_3475 0.64 -0.5 3,941,031 - PP_3475 0.68 -0.7 3,941,150 + -0.7 3,941,150 + -1.4 3,941,150 + +0.6 3,941,151 - -0.5 3,941,151 - -0.4 3,941,154 + +0.5 3,941,172 + -0.5 3,941,172 + +1.1 3,941,172 + +1.0 3,941,173 - -0.4 3,941,173 - +0.6 3,941,322 + PP_3476 0.39 -0.5 3,941,376 + PP_3476 0.53 -1.0 3,941,376 + PP_3476 0.53 -0.7 3,941,376 + PP_3476 0.53 -0.4 3,941,376 + PP_3476 0.53 -0.9 3,941,376 + PP_3476 0.53 -0.5 3,941,377 - PP_3476 0.53 -0.2 3,941,380 - PP_3476 0.54 -0.5 3,941,458 - -0.2 3,941,603 + PP_3477 0.11 +0.0 3,941,603 + PP_3477 0.11 -1.0 3,941,733 - PP_3477 0.38 +0.2 3,941,733 - PP_3477 0.38 -0.4 3,941,776 + PP_3477 0.47 -0.1 3,941,811 + PP_3477 0.54 -0.1 3,941,855 + PP_3477 0.63 -0.1 3,941,882 + PP_3477 0.69 -0.3 3,941,883 - PP_3477 0.69 -0.8 3,941,920 + PP_3477 0.77 -0.1 3,941,921 - PP_3477 0.77 -1.2 3,942,104 + +0.5 3,942,532 - PP_3478 0.27 -0.3 3,942,553 + PP_3478 0.28 +0.3 3,942,554 - PP_3478 0.28 +0.2
Or see this region's nucleotide sequence