Experiment: Isobutyl alcohol 0.6 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3038 and PP_3039 overlap by 4 nucleotides PP_3039 and PP_3040 are separated by 511 nucleotides PP_3040 and PP_3041 are separated by 164 nucleotides PP_3041 and PP_3042 are separated by 4 nucleotides
PP_3038: PP_3038 - Pyocin R2_PP, traC domain protein, at 3,421,843 to 3,424,053
_3038
PP_3039: PP_3039 - conserved protein of unknown function, at 3,424,050 to 3,424,412
_3039
PP_3040: PP_3040 - Pyocin R2_PP, holin, at 3,424,924 to 3,425,271
_3040
PP_3041: PP_3041 - conserved protein of unknown function, at 3,425,436 to 3,426,047
_3041
PP_3042: PP_3042 - putative Terminase, large subunit, at 3,426,052 to 3,428,088
_3042
Position (kb)
3424
3425
3426 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3423.966 kb on + strand at 3423.967 kb on - strand at 3423.982 kb on + strand at 3424.063 kb on + strand at 3424.063 kb on + strand at 3424.063 kb on + strand at 3424.063 kb on + strand at 3424.063 kb on + strand at 3424.063 kb on + strand at 3424.063 kb on + strand at 3424.064 kb on - strand at 3424.064 kb on - strand at 3424.064 kb on - strand at 3424.137 kb on - strand, within PP_3039 at 3424.137 kb on - strand, within PP_3039 at 3424.137 kb on - strand, within PP_3039 at 3424.205 kb on - strand, within PP_3039 at 3424.205 kb on - strand, within PP_3039 at 3424.205 kb on - strand, within PP_3039 at 3424.205 kb on - strand, within PP_3039 at 3424.205 kb on - strand, within PP_3039 at 3424.241 kb on + strand, within PP_3039 at 3424.241 kb on + strand, within PP_3039 at 3424.242 kb on - strand, within PP_3039 at 3424.284 kb on + strand, within PP_3039 at 3424.285 kb on - strand, within PP_3039 at 3424.285 kb on - strand, within PP_3039 at 3424.318 kb on - strand, within PP_3039 at 3424.334 kb on + strand at 3424.343 kb on + strand, within PP_3039 at 3424.344 kb on - strand, within PP_3039 at 3424.344 kb on - strand, within PP_3039 at 3424.347 kb on + strand, within PP_3039 at 3424.347 kb on + strand, within PP_3039 at 3424.347 kb on + strand, within PP_3039 at 3424.347 kb on + strand, within PP_3039 at 3424.348 kb on - strand, within PP_3039 at 3424.350 kb on + strand, within PP_3039 at 3424.350 kb on + strand, within PP_3039 at 3424.350 kb on + strand, within PP_3039 at 3424.351 kb on - strand, within PP_3039 at 3424.351 kb on - strand, within PP_3039 at 3424.360 kb on + strand, within PP_3039 at 3424.361 kb on - strand, within PP_3039 at 3424.379 kb on + strand at 3424.431 kb on - strand at 3424.474 kb on - strand at 3424.518 kb on + strand at 3424.538 kb on + strand at 3424.539 kb on - strand at 3424.539 kb on - strand at 3424.622 kb on + strand at 3424.647 kb on + strand at 3424.691 kb on + strand at 3424.692 kb on - strand at 3424.715 kb on + strand at 3424.716 kb on - strand at 3424.716 kb on - strand at 3424.730 kb on + strand at 3424.731 kb on - strand at 3424.731 kb on - strand at 3424.767 kb on + strand at 3424.774 kb on + strand at 3424.775 kb on - strand at 3424.790 kb on + strand at 3424.791 kb on - strand at 3424.823 kb on + strand at 3424.946 kb on + strand at 3424.946 kb on + strand at 3424.946 kb on + strand at 3424.946 kb on + strand at 3424.947 kb on - strand at 3425.193 kb on - strand, within PP_3040 at 3425.200 kb on + strand, within PP_3040 at 3425.201 kb on - strand, within PP_3040 at 3425.201 kb on - strand at 3425.371 kb on + strand at 3425.415 kb on + strand at 3425.499 kb on + strand, within PP_3041 at 3425.499 kb on + strand, within PP_3041 at 3425.499 kb on + strand, within PP_3041 at 3425.500 kb on - strand, within PP_3041 at 3425.500 kb on - strand, within PP_3041 at 3425.763 kb on + strand, within PP_3041 at 3425.793 kb on + strand, within PP_3041 at 3425.897 kb on - strand, within PP_3041 at 3425.897 kb on - strand, within PP_3041 at 3426.035 kb on + strand at 3426.051 kb on + strand at 3426.051 kb on + strand at 3426.052 kb on - strand at 3426.129 kb on - strand at 3426.129 kb on - strand at 3426.129 kb on - strand at 3426.154 kb on + strand at 3426.154 kb on + strand at 3426.154 kb on + strand at 3426.155 kb on - strand at 3426.213 kb on + strand at 3426.213 kb on + strand at 3426.214 kb on - strand at 3426.220 kb on + strand at 3426.234 kb on + strand at 3426.235 kb on - strand at 3426.235 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Isobutyl alcohol 0.6 vol% remove 3,423,966 + -0.5 3,423,967 - +1.2 3,423,982 + -0.5 3,424,063 + +1.1 3,424,063 + +1.4 3,424,063 + -0.1 3,424,063 + -0.1 3,424,063 + -0.5 3,424,063 + +0.2 3,424,063 + -0.5 3,424,064 - -0.8 3,424,064 - +2.5 3,424,064 - -0.5 3,424,137 - PP_3039 0.24 +1.6 3,424,137 - PP_3039 0.24 +0.3 3,424,137 - PP_3039 0.24 +0.4 3,424,205 - PP_3039 0.43 +1.4 3,424,205 - PP_3039 0.43 -0.4 3,424,205 - PP_3039 0.43 +1.3 3,424,205 - PP_3039 0.43 -1.1 3,424,205 - PP_3039 0.43 -0.9 3,424,241 + PP_3039 0.53 +0.6 3,424,241 + PP_3039 0.53 +0.1 3,424,242 - PP_3039 0.53 -1.9 3,424,284 + PP_3039 0.64 -0.0 3,424,285 - PP_3039 0.65 -0.5 3,424,285 - PP_3039 0.65 -0.7 3,424,318 - PP_3039 0.74 +1.5 3,424,334 + +0.6 3,424,343 + PP_3039 0.81 -0.4 3,424,344 - PP_3039 0.81 +0.4 3,424,344 - PP_3039 0.81 +0.5 3,424,347 + PP_3039 0.82 -0.7 3,424,347 + PP_3039 0.82 -0.9 3,424,347 + PP_3039 0.82 -0.5 3,424,347 + PP_3039 0.82 -0.7 3,424,348 - PP_3039 0.82 -0.7 3,424,350 + PP_3039 0.83 -0.4 3,424,350 + PP_3039 0.83 +0.4 3,424,350 + PP_3039 0.83 -0.3 3,424,351 - PP_3039 0.83 -1.0 3,424,351 - PP_3039 0.83 -0.3 3,424,360 + PP_3039 0.85 -0.5 3,424,361 - PP_3039 0.86 -0.1 3,424,379 + -0.3 3,424,431 - -0.5 3,424,474 - -0.4 3,424,518 + +0.0 3,424,538 + -0.0 3,424,539 - -0.1 3,424,539 - -0.1 3,424,622 + +0.1 3,424,647 + +0.5 3,424,691 + +0.2 3,424,692 - +0.5 3,424,715 + -0.8 3,424,716 - +0.3 3,424,716 - +1.0 3,424,730 + -0.6 3,424,731 - -0.1 3,424,731 - +0.8 3,424,767 + -0.2 3,424,774 + -0.6 3,424,775 - -0.6 3,424,790 + -0.2 3,424,791 - +0.1 3,424,823 + -0.6 3,424,946 + -0.4 3,424,946 + -2.2 3,424,946 + +0.5 3,424,946 + +1.3 3,424,947 - -0.2 3,425,193 - PP_3040 0.77 -0.3 3,425,200 + PP_3040 0.79 -1.0 3,425,201 - PP_3040 0.80 -0.5 3,425,201 - -1.4 3,425,371 + +1.0 3,425,415 + +0.1 3,425,499 + PP_3041 0.10 +0.2 3,425,499 + PP_3041 0.10 +0.6 3,425,499 + PP_3041 0.10 -0.7 3,425,500 - PP_3041 0.10 +0.0 3,425,500 - PP_3041 0.10 +0.2 3,425,763 + PP_3041 0.53 -0.6 3,425,793 + PP_3041 0.58 -0.7 3,425,897 - PP_3041 0.75 +0.6 3,425,897 - PP_3041 0.75 -1.5 3,426,035 + +1.4 3,426,051 + -0.1 3,426,051 + -0.7 3,426,052 - -0.6 3,426,129 - +0.7 3,426,129 - +0.2 3,426,129 - -0.5 3,426,154 + +0.1 3,426,154 + +0.1 3,426,154 + -0.2 3,426,155 - -0.0 3,426,213 + -0.0 3,426,213 + +2.2 3,426,214 - +0.5 3,426,220 + +0.6 3,426,234 + -0.9 3,426,235 - -0.8 3,426,235 - +1.4
Or see this region's nucleotide sequence