Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF741 and GFF742 are separated by 346 nucleotides GFF742 and GFF743 overlap by 4 nucleotides GFF743 and GFF744 are separated by 375 nucleotides
GFF741 - D-beta-hydroxybutyrate permease, at 100,242 to 100,667
GFF741
GFF742 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 101,014 to 101,694
GFF742
GFF743 - Uncharacterized glutathione S-transferase-like protein, at 101,691 to 102,317
GFF743
GFF744 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 102,693 to 103,763
GFF744
Position (kb)
101
102 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 100.177 kb on + strand at 100.177 kb on + strand at 100.178 kb on - strand at 100.178 kb on - strand at 100.178 kb on - strand at 100.295 kb on + strand, within GFF741 at 100.296 kb on - strand, within GFF741 at 100.377 kb on + strand, within GFF741 at 100.480 kb on - strand, within GFF741 at 100.538 kb on + strand, within GFF741 at 100.542 kb on + strand, within GFF741 at 100.678 kb on - strand at 100.801 kb on - strand at 101.010 kb on + strand at 101.140 kb on + strand, within GFF742 at 101.197 kb on + strand, within GFF742 at 101.197 kb on + strand, within GFF742 at 101.197 kb on + strand, within GFF742 at 101.198 kb on - strand, within GFF742 at 101.353 kb on + strand, within GFF742 at 101.354 kb on - strand, within GFF742 at 101.416 kb on + strand, within GFF742 at 101.419 kb on + strand, within GFF742 at 101.596 kb on - strand, within GFF742 at 101.596 kb on - strand, within GFF742 at 101.599 kb on + strand, within GFF742 at 101.599 kb on + strand, within GFF742 at 101.626 kb on + strand, within GFF742 at 101.630 kb on + strand at 101.630 kb on + strand at 101.630 kb on + strand at 101.630 kb on + strand at 101.631 kb on - strand at 101.631 kb on - strand at 101.631 kb on - strand at 101.631 kb on - strand at 101.807 kb on - strand, within GFF743 at 101.822 kb on + strand, within GFF743 at 101.904 kb on - strand, within GFF743 at 102.057 kb on - strand, within GFF743 at 102.127 kb on - strand, within GFF743 at 102.171 kb on - strand, within GFF743 at 102.311 kb on + strand at 102.312 kb on - strand at 102.318 kb on - strand at 102.372 kb on - strand at 102.377 kb on + strand at 102.385 kb on - strand at 102.434 kb on + strand at 102.465 kb on + strand at 102.513 kb on - strand at 102.557 kb on + strand at 102.560 kb on + strand at 102.570 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 100,177 + -1.9 100,177 + +0.1 100,178 - +0.2 100,178 - +0.2 100,178 - -1.1 100,295 + GFF741 0.12 +0.4 100,296 - GFF741 0.13 +0.6 100,377 + GFF741 0.32 +0.2 100,480 - GFF741 0.56 -0.6 100,538 + GFF741 0.69 -0.4 100,542 + GFF741 0.70 +1.9 100,678 - -0.2 100,801 - -0.1 101,010 + -2.3 101,140 + GFF742 0.19 +1.1 101,197 + GFF742 0.27 +0.8 101,197 + GFF742 0.27 +0.9 101,197 + GFF742 0.27 +0.3 101,198 - GFF742 0.27 +1.0 101,353 + GFF742 0.50 +0.8 101,354 - GFF742 0.50 -0.1 101,416 + GFF742 0.59 +0.2 101,419 + GFF742 0.59 -0.4 101,596 - GFF742 0.85 +0.6 101,596 - GFF742 0.85 -0.1 101,599 + GFF742 0.86 -0.5 101,599 + GFF742 0.86 +0.6 101,626 + GFF742 0.90 -0.2 101,630 + -0.1 101,630 + +1.7 101,630 + -0.8 101,630 + -1.3 101,631 - -0.9 101,631 - -0.5 101,631 - -0.4 101,631 - -1.2 101,807 - GFF743 0.19 -0.3 101,822 + GFF743 0.21 -0.5 101,904 - GFF743 0.34 -0.6 102,057 - GFF743 0.58 +0.4 102,127 - GFF743 0.70 +0.1 102,171 - GFF743 0.77 +0.1 102,311 + -0.0 102,312 - +0.1 102,318 - -0.5 102,372 - -0.8 102,377 + -0.2 102,385 - +0.1 102,434 + -0.3 102,465 + +0.3 102,513 - -1.7 102,557 + +0.1 102,560 + +0.0 102,570 + +0.5
Or see this region's nucleotide sequence