Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF576

Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF575 and GFF576 overlap by 4 nucleotidesGFF576 and GFF577 are separated by 14 nucleotides GFF575 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 304,797 to 306,983 GFF575 GFF576 - Glycogen debranching enzyme (EC 3.2.1.-), at 306,980 to 308,956 GFF576 GFF577 - Glucose-1-phosphate adenylyltransferase (EC 2.7.7.27), at 308,971 to 310,266 GFF577 Position (kb) 306 307 308 309Strain fitness (log2 ratio) -2 -1 0 1 2at 306.023 kb on + strand, within GFF575at 306.030 kb on + strand, within GFF575at 306.119 kb on + strand, within GFF575at 306.231 kb on + strand, within GFF575at 306.333 kb on + strand, within GFF575at 306.454 kb on - strand, within GFF575at 306.454 kb on - strand, within GFF575at 306.455 kb on + strand, within GFF575at 306.456 kb on + strand, within GFF575at 306.457 kb on - strand, within GFF575at 306.624 kb on + strand, within GFF575at 306.635 kb on + strand, within GFF575at 306.636 kb on - strand, within GFF575at 306.636 kb on - strand, within GFF575at 306.767 kb on + strandat 306.929 kb on + strandat 307.025 kb on + strandat 307.026 kb on - strandat 307.026 kb on - strandat 307.026 kb on - strandat 307.077 kb on + strandat 307.077 kb on + strandat 307.290 kb on + strand, within GFF576at 307.385 kb on + strand, within GFF576at 307.386 kb on - strand, within GFF576at 307.440 kb on - strand, within GFF576at 307.466 kb on + strand, within GFF576at 307.494 kb on + strand, within GFF576at 307.505 kb on + strand, within GFF576at 307.505 kb on + strand, within GFF576at 307.642 kb on + strand, within GFF576at 307.642 kb on + strand, within GFF576at 307.643 kb on - strand, within GFF576at 307.768 kb on - strand, within GFF576at 307.770 kb on + strand, within GFF576at 307.834 kb on + strand, within GFF576at 307.838 kb on + strand, within GFF576at 307.838 kb on + strand, within GFF576at 307.838 kb on + strand, within GFF576at 307.839 kb on - strand, within GFF576at 307.839 kb on - strand, within GFF576at 307.839 kb on - strand, within GFF576at 307.841 kb on + strand, within GFF576at 308.396 kb on + strand, within GFF576at 308.396 kb on + strand, within GFF576at 308.443 kb on - strand, within GFF576at 308.443 kb on - strand, within GFF576at 308.557 kb on + strand, within GFF576at 308.671 kb on - strand, within GFF576at 308.680 kb on + strand, within GFF576at 308.681 kb on - strand, within GFF576at 308.767 kb on + strandat 308.767 kb on + strandat 308.768 kb on - strandat 308.836 kb on + strandat 308.836 kb on + strandat 308.836 kb on + strandat 308.836 kb on + strandat 308.839 kb on - strandat 308.982 kb on - strandat 309.000 kb on - strandat 309.038 kb on + strandat 309.039 kb on - strandat 309.144 kb on - strand, within GFF577at 309.193 kb on + strand, within GFF577at 309.193 kb on + strand, within GFF577at 309.220 kb on - strand, within GFF577at 309.291 kb on + strand, within GFF577at 309.351 kb on + strand, within GFF577at 309.351 kb on + strand, within GFF577at 309.352 kb on - strand, within GFF577at 309.374 kb on - strand, within GFF577at 309.400 kb on - strand, within GFF577at 309.400 kb on - strand, within GFF577at 309.403 kb on + strand, within GFF577at 309.404 kb on - strand, within GFF577at 309.424 kb on - strand, within GFF577at 309.424 kb on - strand, within GFF577at 309.472 kb on - strand, within GFF577at 309.640 kb on + strand, within GFF577at 309.641 kb on - strand, within GFF577at 309.800 kb on + strand, within GFF577at 309.800 kb on + strand, within GFF577at 309.801 kb on - strand, within GFF577at 309.801 kb on - strand, within GFF577at 309.804 kb on + strand, within GFF577at 309.868 kb on + strand, within GFF577at 309.868 kb on + strand, within GFF577at 309.868 kb on + strand, within GFF577at 309.868 kb on + strand, within GFF577at 309.869 kb on - strand, within GFF577at 309.869 kb on - strand, within GFF577at 309.869 kb on - strand, within GFF577at 309.869 kb on - strand, within GFF577at 309.891 kb on + strand, within GFF577at 309.892 kb on - strand, within GFF577at 309.898 kb on - strand, within GFF577at 309.951 kb on + strand, within GFF577

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI
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306,023 + GFF575 0.56 +0.4
306,030 + GFF575 0.56 -0.7
306,119 + GFF575 0.60 -0.3
306,231 + GFF575 0.66 +0.4
306,333 + GFF575 0.70 +0.1
306,454 - GFF575 0.76 +0.1
306,454 - GFF575 0.76 -1.2
306,455 + GFF575 0.76 -0.1
306,456 + GFF575 0.76 +0.7
306,457 - GFF575 0.76 +0.4
306,624 + GFF575 0.84 +0.0
306,635 + GFF575 0.84 +0.3
306,636 - GFF575 0.84 +0.3
306,636 - GFF575 0.84 -0.3
306,767 + -0.1
306,929 + -1.6
307,025 + -0.2
307,026 - +0.6
307,026 - +0.7
307,026 - -0.7
307,077 + +0.2
307,077 + +0.6
307,290 + GFF576 0.16 -1.3
307,385 + GFF576 0.20 -0.0
307,386 - GFF576 0.21 -0.4
307,440 - GFF576 0.23 -0.1
307,466 + GFF576 0.25 +0.3
307,494 + GFF576 0.26 +0.5
307,505 + GFF576 0.27 +0.0
307,505 + GFF576 0.27 +0.7
307,642 + GFF576 0.33 +0.6
307,642 + GFF576 0.33 -0.4
307,643 - GFF576 0.34 -1.5
307,768 - GFF576 0.40 -1.6
307,770 + GFF576 0.40 -0.3
307,834 + GFF576 0.43 +0.4
307,838 + GFF576 0.43 +0.6
307,838 + GFF576 0.43 +1.1
307,838 + GFF576 0.43 +0.6
307,839 - GFF576 0.43 +0.4
307,839 - GFF576 0.43 -0.3
307,839 - GFF576 0.43 +0.7
307,841 + GFF576 0.44 +0.4
308,396 + GFF576 0.72 +0.1
308,396 + GFF576 0.72 -2.6
308,443 - GFF576 0.74 -0.1
308,443 - GFF576 0.74 +0.4
308,557 + GFF576 0.80 +0.5
308,671 - GFF576 0.86 +0.1
308,680 + GFF576 0.86 +1.0
308,681 - GFF576 0.86 +0.0
308,767 + -0.6
308,767 + +0.3
308,768 - +0.7
308,836 + +0.2
308,836 + +0.3
308,836 + -1.2
308,836 + +0.0
308,839 - +0.5
308,982 - +0.1
309,000 - -0.3
309,038 + -0.7
309,039 - -0.1
309,144 - GFF577 0.13 -0.3
309,193 + GFF577 0.17 +0.2
309,193 + GFF577 0.17 -0.1
309,220 - GFF577 0.19 +0.1
309,291 + GFF577 0.25 +0.2
309,351 + GFF577 0.29 -0.5
309,351 + GFF577 0.29 -0.4
309,352 - GFF577 0.29 +0.1
309,374 - GFF577 0.31 +0.9
309,400 - GFF577 0.33 -0.5
309,400 - GFF577 0.33 +0.2
309,403 + GFF577 0.33 +1.4
309,404 - GFF577 0.33 +0.6
309,424 - GFF577 0.35 +1.8
309,424 - GFF577 0.35 +0.7
309,472 - GFF577 0.39 +0.0
309,640 + GFF577 0.52 +0.2
309,641 - GFF577 0.52 +0.6
309,800 + GFF577 0.64 +0.1
309,800 + GFF577 0.64 +0.1
309,801 - GFF577 0.64 +0.2
309,801 - GFF577 0.64 +0.0
309,804 + GFF577 0.64 +0.0
309,868 + GFF577 0.69 +0.7
309,868 + GFF577 0.69 +0.1
309,868 + GFF577 0.69 +0.9
309,868 + GFF577 0.69 +0.7
309,869 - GFF577 0.69 -0.1
309,869 - GFF577 0.69 -0.4
309,869 - GFF577 0.69 -0.0
309,869 - GFF577 0.69 +0.0
309,891 + GFF577 0.71 +0.8
309,892 - GFF577 0.71 +0.3
309,898 - GFF577 0.72 +0.2
309,951 + GFF577 0.76 +1.7

Or see this region's nucleotide sequence