Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4834 and GFF4835 are separated by 767 nucleotides GFF4835 and GFF4836 overlap by 29 nucleotides GFF4836 and GFF4837 are separated by 152 nucleotides
GFF4834 - Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8), at 130,576 to 131,376
GFF4834
GFF4835 - ParE toxin protein, at 132,144 to 132,434
GFF4835
GFF4836 - Transcriptional regulator, ArsR family, at 132,406 to 132,693
GFF4836
GFF4837 - 'GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)' transl_table=11, at 132,846 to 135,080
GFF4837
Position (kb) 132
133 Strain fitness (log2 ratio) -2
-1
0
1 at 131.247 kb on - strand, within GFF4834 at 131.247 kb on - strand, within GFF4834 at 131.263 kb on - strand, within GFF4834 at 131.368 kb on - strand at 131.394 kb on - strand at 131.913 kb on + strand at 131.955 kb on + strand at 132.191 kb on + strand, within GFF4835 at 132.191 kb on + strand, within GFF4835 at 132.330 kb on + strand, within GFF4835 at 132.708 kb on + strand at 132.769 kb on + strand at 132.791 kb on + strand at 132.791 kb on + strand at 132.826 kb on - strand at 132.947 kb on + strand at 133.122 kb on + strand, within GFF4837 at 133.386 kb on - strand, within GFF4837
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 131,247 - GFF4834 0.84 +0.2 131,247 - GFF4834 0.84 +0.5 131,263 - GFF4834 0.86 +0.2 131,368 - +0.0 131,394 - -0.2 131,913 + +0.2 131,955 + +0.1 132,191 + GFF4835 0.16 -0.3 132,191 + GFF4835 0.16 -0.5 132,330 + GFF4835 0.64 -1.8 132,708 + +0.6 132,769 + +0.6 132,791 + -0.1 132,791 + -0.0 132,826 - +0.6 132,947 + +0.1 133,122 + GFF4837 0.12 +0.0 133,386 - GFF4837 0.24 -1.0
Or see this region's nucleotide sequence