Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4820 and GFF4821 are separated by 324 nucleotides GFF4821 and GFF4822 are separated by 30 nucleotides GFF4822 and GFF4823 are separated by 87 nucleotides GFF4823 and GFF4824 overlap by 2 nucleotides
GFF4820 - Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2), at 120,677 to 122,476
GFF4820
GFF4821 - FIG01047384: hypothetical protein, at 122,801 to 122,971
GFF4821
GFF4822 - Queuosine biosynthesis QueD, PTPS-I, at 123,002 to 123,262
GFF4822
GFF4823 - STY3078 protein, at 123,350 to 124,147
GFF4823
GFF4824 - FIG01046827: hypothetical protein, at 124,146 to 124,400
GFF4824
Position (kb) 123
124 Strain fitness (log2 ratio) -1
0
1
2
3 at 122.278 kb on + strand, within GFF4820 at 122.278 kb on + strand, within GFF4820 at 122.484 kb on + strand at 122.561 kb on - strand at 122.561 kb on - strand at 122.917 kb on + strand, within GFF4821 at 122.921 kb on + strand, within GFF4821 at 122.972 kb on - strand at 123.092 kb on + strand, within GFF4822 at 123.114 kb on - strand, within GFF4822 at 123.575 kb on - strand, within GFF4823 at 123.634 kb on - strand, within GFF4823 at 123.793 kb on - strand, within GFF4823 at 123.934 kb on - strand, within GFF4823 at 124.037 kb on - strand, within GFF4823 at 124.037 kb on - strand, within GFF4823 at 124.128 kb on - strand at 124.208 kb on + strand, within GFF4824 at 124.239 kb on + strand, within GFF4824 at 124.251 kb on - strand, within GFF4824
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 122,278 + GFF4820 0.89 +3.2 122,278 + GFF4820 0.89 +0.3 122,484 + +1.0 122,561 - +0.3 122,561 - +0.3 122,917 + GFF4821 0.68 +0.2 122,921 + GFF4821 0.70 +0.3 122,972 - +0.4 123,092 + GFF4822 0.34 -0.9 123,114 - GFF4822 0.43 +0.4 123,575 - GFF4823 0.28 +0.2 123,634 - GFF4823 0.36 -0.1 123,793 - GFF4823 0.56 +0.0 123,934 - GFF4823 0.73 +0.5 124,037 - GFF4823 0.86 +0.3 124,037 - GFF4823 0.86 -0.7 124,128 - -1.4 124,208 + GFF4824 0.24 -0.0 124,239 + GFF4824 0.36 +0.1 124,251 - GFF4824 0.41 -0.0
Or see this region's nucleotide sequence