Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4603 and GFF4604 overlap by 4 nucleotides GFF4604 and GFF4605 are separated by 397 nucleotides GFF4605 and GFF4606 are separated by 250 nucleotides
GFF4603 - Putrescine/proton symporter, putrescine/ornithine antiporter PotE, at 392,607 to 393,926
GFF4603
GFF4604 - Ornithine decarboxylase (EC 4.1.1.17), at 393,923 to 396,121
GFF4604
GFF4605 - hypothetical protein, at 396,519 to 396,632
GFF4605
GFF4606 - sensor histidine kinase, at 396,883 to 397,560
GFF4606
Position (kb) 393
394
395
396
397 Strain fitness (log2 ratio) -2
-1
0
1 at 393.215 kb on - strand, within GFF4603 at 393.215 kb on - strand, within GFF4603 at 393.327 kb on + strand, within GFF4603 at 393.393 kb on + strand, within GFF4603 at 393.397 kb on + strand, within GFF4603 at 393.397 kb on + strand, within GFF4603 at 393.397 kb on + strand, within GFF4603 at 393.398 kb on - strand, within GFF4603 at 393.398 kb on - strand, within GFF4603 at 393.398 kb on - strand, within GFF4603 at 393.476 kb on + strand, within GFF4603 at 393.477 kb on - strand, within GFF4603 at 393.494 kb on + strand, within GFF4603 at 393.497 kb on - strand, within GFF4603 at 393.589 kb on + strand, within GFF4603 at 393.656 kb on + strand, within GFF4603 at 393.658 kb on + strand, within GFF4603 at 393.659 kb on - strand, within GFF4603 at 393.738 kb on - strand, within GFF4603 at 393.800 kb on + strand at 393.872 kb on + strand at 393.873 kb on - strand at 393.920 kb on + strand at 394.018 kb on - strand at 394.122 kb on - strand at 394.122 kb on - strand at 394.219 kb on - strand, within GFF4604 at 394.219 kb on - strand, within GFF4604 at 394.224 kb on + strand, within GFF4604 at 394.224 kb on + strand, within GFF4604 at 394.225 kb on - strand, within GFF4604 at 394.225 kb on - strand, within GFF4604 at 394.225 kb on - strand, within GFF4604 at 394.246 kb on + strand, within GFF4604 at 394.315 kb on - strand, within GFF4604 at 394.351 kb on + strand, within GFF4604 at 394.394 kb on + strand, within GFF4604 at 394.466 kb on + strand, within GFF4604 at 394.865 kb on - strand, within GFF4604 at 395.012 kb on - strand, within GFF4604 at 395.083 kb on + strand, within GFF4604 at 395.184 kb on + strand, within GFF4604 at 395.230 kb on - strand, within GFF4604 at 395.245 kb on - strand, within GFF4604 at 395.377 kb on - strand, within GFF4604 at 395.646 kb on - strand, within GFF4604 at 395.769 kb on + strand, within GFF4604 at 395.770 kb on - strand, within GFF4604 at 395.848 kb on - strand, within GFF4604 at 396.456 kb on - strand at 396.554 kb on + strand, within GFF4605 at 396.922 kb on - strand at 396.977 kb on + strand, within GFF4606 at 396.977 kb on + strand, within GFF4606 at 396.978 kb on - strand, within GFF4606 at 397.030 kb on + strand, within GFF4606 at 397.065 kb on + strand, within GFF4606 at 397.065 kb on + strand, within GFF4606
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 393,215 - GFF4603 0.46 +0.1 393,215 - GFF4603 0.46 -2.1 393,327 + GFF4603 0.55 +1.6 393,393 + GFF4603 0.60 -1.3 393,397 + GFF4603 0.60 +0.6 393,397 + GFF4603 0.60 +0.1 393,397 + GFF4603 0.60 -0.2 393,398 - GFF4603 0.60 -0.2 393,398 - GFF4603 0.60 -0.0 393,398 - GFF4603 0.60 -0.1 393,476 + GFF4603 0.66 -0.1 393,477 - GFF4603 0.66 -0.1 393,494 + GFF4603 0.67 -0.4 393,497 - GFF4603 0.67 -1.1 393,589 + GFF4603 0.74 -0.2 393,656 + GFF4603 0.79 -0.4 393,658 + GFF4603 0.80 +0.6 393,659 - GFF4603 0.80 -0.4 393,738 - GFF4603 0.86 +1.0 393,800 + -0.9 393,872 + -0.2 393,873 - -1.3 393,920 + -1.7 394,018 - -0.4 394,122 - +1.0 394,122 - +1.0 394,219 - GFF4604 0.13 -0.2 394,219 - GFF4604 0.13 -0.3 394,224 + GFF4604 0.14 -0.4 394,224 + GFF4604 0.14 -0.7 394,225 - GFF4604 0.14 -0.2 394,225 - GFF4604 0.14 +0.2 394,225 - GFF4604 0.14 -0.3 394,246 + GFF4604 0.15 +1.4 394,315 - GFF4604 0.18 -1.1 394,351 + GFF4604 0.19 -0.9 394,394 + GFF4604 0.21 -1.4 394,466 + GFF4604 0.25 +0.6 394,865 - GFF4604 0.43 -0.1 395,012 - GFF4604 0.50 -0.3 395,083 + GFF4604 0.53 +0.4 395,184 + GFF4604 0.57 -0.6 395,230 - GFF4604 0.59 -0.7 395,245 - GFF4604 0.60 +0.9 395,377 - GFF4604 0.66 -1.4 395,646 - GFF4604 0.78 -0.0 395,769 + GFF4604 0.84 -2.6 395,770 - GFF4604 0.84 +0.3 395,848 - GFF4604 0.88 -0.5 396,456 - -0.4 396,554 + GFF4605 0.31 +0.7 396,922 - +0.1 396,977 + GFF4606 0.14 +1.0 396,977 + GFF4606 0.14 -0.5 396,978 - GFF4606 0.14 -0.2 397,030 + GFF4606 0.22 +0.3 397,065 + GFF4606 0.27 +1.6 397,065 + GFF4606 0.27 +0.0
Or see this region's nucleotide sequence