Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4379 and GFF4380 are separated by 22 nucleotides GFF4380 and GFF4381 are separated by 141 nucleotides GFF4381 and GFF4382 are separated by 124 nucleotides
GFF4379 - RND efflux system, inner membrane transporter CmeB, at 159,461 to 162,610
GFF4379
GFF4380 - Membrane fusion protein of RND family multidrug efflux pump, at 162,633 to 163,826
GFF4380
GFF4381 - Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family, at 163,968 to 164,621
GFF4381
GFF4382 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 164,746 to 168,102
GFF4382
Position (kb) 162
163
164 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 162.182 kb on - strand, within GFF4379 at 162.209 kb on - strand, within GFF4379 at 162.246 kb on - strand, within GFF4379 at 162.379 kb on + strand at 162.380 kb on - strand at 162.380 kb on - strand at 162.380 kb on - strand at 162.380 kb on - strand at 162.380 kb on - strand at 162.380 kb on - strand at 162.381 kb on + strand at 162.381 kb on + strand at 162.381 kb on + strand at 162.381 kb on + strand at 162.382 kb on - strand at 162.382 kb on - strand at 162.382 kb on - strand at 162.382 kb on - strand at 162.434 kb on - strand at 162.634 kb on + strand at 162.708 kb on - strand at 162.727 kb on - strand at 162.967 kb on - strand, within GFF4380 at 163.005 kb on - strand, within GFF4380 at 163.007 kb on + strand, within GFF4380 at 163.189 kb on + strand, within GFF4380 at 163.190 kb on - strand, within GFF4380 at 163.193 kb on + strand, within GFF4380 at 163.193 kb on + strand, within GFF4380 at 163.278 kb on - strand, within GFF4380 at 163.309 kb on + strand, within GFF4380 at 163.323 kb on - strand, within GFF4380 at 163.323 kb on - strand, within GFF4380 at 163.502 kb on + strand, within GFF4380 at 163.513 kb on + strand, within GFF4380 at 163.589 kb on + strand, within GFF4380 at 163.602 kb on - strand, within GFF4380 at 163.639 kb on + strand, within GFF4380 at 163.640 kb on - strand, within GFF4380 at 163.898 kb on - strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.928 kb on + strand at 163.929 kb on - strand at 163.934 kb on + strand at 163.935 kb on - strand at 163.967 kb on + strand at 164.113 kb on - strand, within GFF4381 at 164.141 kb on - strand, within GFF4381 at 164.231 kb on - strand, within GFF4381 at 164.291 kb on - strand, within GFF4381 at 164.304 kb on - strand, within GFF4381 at 164.411 kb on + strand, within GFF4381 at 164.571 kb on + strand at 164.572 kb on - strand at 164.572 kb on - strand at 164.626 kb on - strand at 164.746 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 162,182 - GFF4379 0.86 +0.4 162,209 - GFF4379 0.87 +0.4 162,246 - GFF4379 0.88 -1.3 162,379 + +0.2 162,380 - -0.1 162,380 - -0.3 162,380 - +0.2 162,380 - -0.8 162,380 - +0.1 162,380 - -0.1 162,381 + -0.0 162,381 + -0.9 162,381 + +0.1 162,381 + -0.5 162,382 - -1.0 162,382 - +0.2 162,382 - -0.8 162,382 - +0.3 162,434 - -0.7 162,634 + -1.1 162,708 - -0.1 162,727 - +0.4 162,967 - GFF4380 0.28 -0.6 163,005 - GFF4380 0.31 -1.1 163,007 + GFF4380 0.31 -0.5 163,189 + GFF4380 0.47 -0.2 163,190 - GFF4380 0.47 +1.0 163,193 + GFF4380 0.47 +0.1 163,193 + GFF4380 0.47 -0.7 163,278 - GFF4380 0.54 -1.6 163,309 + GFF4380 0.57 -0.4 163,323 - GFF4380 0.58 -0.0 163,323 - GFF4380 0.58 -0.9 163,502 + GFF4380 0.73 +0.6 163,513 + GFF4380 0.74 -0.8 163,589 + GFF4380 0.80 -2.9 163,602 - GFF4380 0.81 +0.5 163,639 + GFF4380 0.84 -1.5 163,640 - GFF4380 0.84 -0.8 163,898 - +0.5 163,920 + -0.5 163,920 + -3.2 163,920 + -1.5 163,920 + -1.5 163,920 + -0.1 163,920 + +0.1 163,921 - +0.5 163,921 - -0.8 163,921 - +0.8 163,921 - -0.1 163,921 - +0.3 163,921 - +0.3 163,928 + -0.9 163,929 - +1.4 163,934 + -0.2 163,935 - +0.2 163,967 + +0.1 164,113 - GFF4381 0.22 +0.2 164,141 - GFF4381 0.26 -0.2 164,231 - GFF4381 0.40 -0.0 164,291 - GFF4381 0.49 +0.4 164,304 - GFF4381 0.51 +0.4 164,411 + GFF4381 0.68 -0.1 164,571 + +1.3 164,572 - +0.5 164,572 - +0.0 164,626 - +0.1 164,746 - +0.2
Or see this region's nucleotide sequence