Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4376 and GFF4377 are separated by 178 nucleotides GFF4377 and GFF4378 are separated by 27 nucleotides GFF4378 and GFF4379 are separated by 495 nucleotides
GFF4376 - Maltose O-acetyltransferase (EC 2.3.1.79), at 157,615 to 158,166
GFF4376
GFF4377 - Haemolysin expression modulating protein, at 158,345 to 158,563
GFF4377
GFF4378 - FIG00948312: hypothetical protein, at 158,591 to 158,965
GFF4378
GFF4379 - RND efflux system, inner membrane transporter CmeB, at 159,461 to 162,610
GFF4379
Position (kb) 158
159 Strain fitness (log2 ratio) -2
-1
0
1
2 at 157.734 kb on + strand, within GFF4376 at 157.789 kb on + strand, within GFF4376 at 157.842 kb on - strand, within GFF4376 at 157.842 kb on - strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.850 kb on - strand, within GFF4376 at 157.984 kb on - strand, within GFF4376 at 158.057 kb on + strand, within GFF4376 at 158.057 kb on + strand, within GFF4376 at 158.058 kb on - strand, within GFF4376 at 158.063 kb on + strand, within GFF4376 at 158.253 kb on - strand at 158.254 kb on + strand at 158.333 kb on - strand at 158.337 kb on + strand at 158.698 kb on - strand, within GFF4378 at 158.785 kb on + strand, within GFF4378 at 158.803 kb on + strand, within GFF4378 at 158.934 kb on - strand at 158.936 kb on - strand at 158.936 kb on - strand at 159.018 kb on - strand at 159.023 kb on + strand at 159.023 kb on + strand at 159.755 kb on + strand at 159.779 kb on - strand, within GFF4379 at 159.782 kb on + strand, within GFF4379 at 159.783 kb on - strand, within GFF4379 at 159.834 kb on + strand, within GFF4379 at 159.835 kb on - strand, within GFF4379 at 159.835 kb on - strand, within GFF4379 at 159.922 kb on + strand, within GFF4379 at 159.936 kb on + strand, within GFF4379 at 159.937 kb on - strand, within GFF4379
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 157,734 + GFF4376 0.22 +0.5 157,789 + GFF4376 0.32 -1.2 157,842 - GFF4376 0.41 -1.9 157,842 - GFF4376 0.41 -0.5 157,845 + GFF4376 0.42 -0.0 157,845 + GFF4376 0.42 -1.2 157,845 + GFF4376 0.42 -0.6 157,845 + GFF4376 0.42 -0.4 157,845 + GFF4376 0.42 -0.9 157,846 - GFF4376 0.42 +1.9 157,846 - GFF4376 0.42 +0.5 157,846 - GFF4376 0.42 -0.6 157,846 - GFF4376 0.42 +2.4 157,846 - GFF4376 0.42 -0.0 157,846 - GFF4376 0.42 -0.1 157,846 - GFF4376 0.42 -0.9 157,846 - GFF4376 0.42 -0.5 157,846 - GFF4376 0.42 -0.1 157,846 - GFF4376 0.42 -0.4 157,850 - GFF4376 0.43 -0.6 157,984 - GFF4376 0.67 +0.6 158,057 + GFF4376 0.80 -0.4 158,057 + GFF4376 0.80 -0.4 158,058 - GFF4376 0.80 -1.5 158,063 + GFF4376 0.81 +0.1 158,253 - -0.5 158,254 + +0.1 158,333 - -0.6 158,337 + +0.5 158,698 - GFF4378 0.29 -0.9 158,785 + GFF4378 0.52 -1.0 158,803 + GFF4378 0.57 +0.6 158,934 - -0.1 158,936 - -0.4 158,936 - +0.7 159,018 - +0.1 159,023 + +0.7 159,023 + +0.7 159,755 + -1.5 159,779 - GFF4379 0.10 +2.2 159,782 + GFF4379 0.10 +1.0 159,783 - GFF4379 0.10 +0.8 159,834 + GFF4379 0.12 -0.2 159,835 - GFF4379 0.12 +0.1 159,835 - GFF4379 0.12 -0.0 159,922 + GFF4379 0.15 -0.2 159,936 + GFF4379 0.15 -0.1 159,937 - GFF4379 0.15 -0.1
Or see this region's nucleotide sequence