Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4361

Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4359 and GFF4360 are separated by 105 nucleotidesGFF4360 and GFF4361 are separated by 64 nucleotidesGFF4361 and GFF4362 are separated by 100 nucleotidesGFF4362 and GFF4363 are separated by 49 nucleotides GFF4359 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 141,379 to 141,777 GFF4359 GFF4360 - Queuosine Biosynthesis QueC ATPase, at 141,883 to 142,578 GFF4360 GFF4361 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 142,643 to 144,343 GFF4361 GFF4362 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 144,444 to 145,262 GFF4362 GFF4363 - Cysteine synthase B (EC 2.5.1.47), at 145,312 to 146,367 GFF4363 Position (kb) 142 143 144 145Strain fitness (log2 ratio) -3 -2 -1 0 1at 141.821 kb on - strandat 141.836 kb on + strandat 141.890 kb on - strandat 142.003 kb on - strand, within GFF4360at 142.087 kb on - strand, within GFF4360at 142.099 kb on + strand, within GFF4360at 142.506 kb on - strand, within GFF4360at 142.559 kb on - strandat 142.579 kb on - strandat 142.579 kb on - strandat 142.579 kb on - strandat 142.582 kb on + strandat 142.750 kb on + strandat 142.917 kb on - strand, within GFF4361at 142.933 kb on + strand, within GFF4361at 143.096 kb on + strand, within GFF4361at 143.109 kb on + strand, within GFF4361at 143.201 kb on - strand, within GFF4361at 143.242 kb on + strand, within GFF4361at 143.254 kb on + strand, within GFF4361at 143.255 kb on - strand, within GFF4361at 143.255 kb on - strand, within GFF4361at 143.258 kb on + strand, within GFF4361at 143.280 kb on - strand, within GFF4361at 143.313 kb on + strand, within GFF4361at 143.333 kb on - strand, within GFF4361at 143.338 kb on + strand, within GFF4361at 143.338 kb on + strand, within GFF4361at 143.339 kb on - strand, within GFF4361at 143.339 kb on - strand, within GFF4361at 143.342 kb on + strand, within GFF4361at 143.342 kb on + strand, within GFF4361at 143.343 kb on - strand, within GFF4361at 143.484 kb on + strand, within GFF4361at 143.589 kb on + strand, within GFF4361at 143.590 kb on - strand, within GFF4361at 143.590 kb on - strand, within GFF4361at 143.710 kb on + strand, within GFF4361at 143.764 kb on + strand, within GFF4361at 143.962 kb on - strand, within GFF4361at 143.986 kb on + strand, within GFF4361at 144.066 kb on - strand, within GFF4361at 144.335 kb on + strandat 144.381 kb on - strandat 144.468 kb on - strandat 144.481 kb on + strandat 144.484 kb on + strandat 144.485 kb on - strandat 144.625 kb on - strand, within GFF4362at 145.243 kb on + strandat 145.243 kb on + strandat 145.244 kb on - strandat 145.244 kb on - strandat 145.249 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI
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141,821 - -0.3
141,836 + +0.4
141,890 - -0.0
142,003 - GFF4360 0.17 -0.2
142,087 - GFF4360 0.29 -0.2
142,099 + GFF4360 0.31 -0.2
142,506 - GFF4360 0.90 -0.1
142,559 - +0.3
142,579 - -0.1
142,579 - +0.7
142,579 - -0.1
142,582 + +0.9
142,750 + +0.1
142,917 - GFF4361 0.16 -2.1
142,933 + GFF4361 0.17 -0.9
143,096 + GFF4361 0.27 -1.1
143,109 + GFF4361 0.27 -0.2
143,201 - GFF4361 0.33 +0.6
143,242 + GFF4361 0.35 -2.8
143,254 + GFF4361 0.36 -0.3
143,255 - GFF4361 0.36 +0.3
143,255 - GFF4361 0.36 -0.3
143,258 + GFF4361 0.36 -0.9
143,280 - GFF4361 0.37 +0.1
143,313 + GFF4361 0.39 +0.7
143,333 - GFF4361 0.41 -0.5
143,338 + GFF4361 0.41 +0.2
143,338 + GFF4361 0.41 +1.0
143,339 - GFF4361 0.41 -1.4
143,339 - GFF4361 0.41 -0.9
143,342 + GFF4361 0.41 -0.4
143,342 + GFF4361 0.41 +0.3
143,343 - GFF4361 0.41 -0.1
143,484 + GFF4361 0.49 +0.1
143,589 + GFF4361 0.56 -0.2
143,590 - GFF4361 0.56 -0.1
143,590 - GFF4361 0.56 -1.1
143,710 + GFF4361 0.63 +0.3
143,764 + GFF4361 0.66 +0.1
143,962 - GFF4361 0.78 -1.1
143,986 + GFF4361 0.79 -0.8
144,066 - GFF4361 0.84 +1.1
144,335 + +1.2
144,381 - -0.1
144,468 - +0.1
144,481 + +0.5
144,484 + +1.0
144,485 - +0.5
144,625 - GFF4362 0.22 +0.9
145,243 + +0.7
145,243 + +0.3
145,244 - +1.7
145,244 - +0.2
145,249 - -0.2

Or see this region's nucleotide sequence