Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4355 and GFF4356 are separated by 208 nucleotides GFF4356 and GFF4357 are separated by 290 nucleotides GFF4357 and GFF4358 are separated by 149 nucleotides GFF4358 and GFF4359 are separated by 103 nucleotides
GFF4355 - ATP-dependent protease La (EC 3.4.21.53) Type I, at 135,754 to 138,108
GFF4355
GFF4356 - DNA-binding protein HU-beta, at 138,317 to 138,589
GFF4356
GFF4357 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 138,880 to 140,751
GFF4357
GFF4358 - FIG01200701: possible membrane protein, at 140,901 to 141,275
GFF4358
GFF4359 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 141,379 to 141,777
GFF4359
Position (kb)
138
139
140
141 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 137.947 kb on - strand at 138.179 kb on + strand at 138.186 kb on - strand at 138.390 kb on + strand, within GFF4356 at 138.401 kb on - strand, within GFF4356 at 138.582 kb on + strand at 138.587 kb on + strand at 138.620 kb on - strand at 138.763 kb on + strand at 138.848 kb on + strand at 139.000 kb on + strand at 139.068 kb on + strand, within GFF4357 at 139.074 kb on + strand, within GFF4357 at 139.272 kb on + strand, within GFF4357 at 139.359 kb on + strand, within GFF4357 at 139.436 kb on + strand, within GFF4357 at 139.470 kb on + strand, within GFF4357 at 139.543 kb on - strand, within GFF4357 at 139.577 kb on + strand, within GFF4357 at 139.582 kb on + strand, within GFF4357 at 139.582 kb on + strand, within GFF4357 at 139.642 kb on + strand, within GFF4357 at 139.642 kb on + strand, within GFF4357 at 139.642 kb on + strand, within GFF4357 at 139.892 kb on + strand, within GFF4357 at 140.032 kb on + strand, within GFF4357 at 140.276 kb on + strand, within GFF4357 at 140.355 kb on + strand, within GFF4357 at 140.915 kb on + strand at 141.183 kb on + strand, within GFF4358 at 141.251 kb on + strand at 141.273 kb on + strand at 141.274 kb on - strand at 141.336 kb on + strand at 141.337 kb on - strand at 141.347 kb on + strand at 141.352 kb on - strand at 141.354 kb on - strand at 141.354 kb on - strand at 141.365 kb on - strand at 141.551 kb on + strand, within GFF4359 at 141.584 kb on + strand, within GFF4359 at 141.585 kb on - strand, within GFF4359 at 141.632 kb on + strand, within GFF4359
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 137,947 - -0.3 138,179 + -0.4 138,186 - -0.1 138,390 + GFF4356 0.27 -0.1 138,401 - GFF4356 0.31 +0.1 138,582 + -0.1 138,587 + +0.3 138,620 - +0.0 138,763 + +0.1 138,848 + -0.1 139,000 + -0.1 139,068 + GFF4357 0.10 -1.3 139,074 + GFF4357 0.10 +0.1 139,272 + GFF4357 0.21 -0.4 139,359 + GFF4357 0.26 -0.3 139,436 + GFF4357 0.30 -0.5 139,470 + GFF4357 0.32 -0.5 139,543 - GFF4357 0.35 -3.8 139,577 + GFF4357 0.37 +0.1 139,582 + GFF4357 0.38 -1.7 139,582 + GFF4357 0.38 +0.1 139,642 + GFF4357 0.41 -0.2 139,642 + GFF4357 0.41 -0.4 139,642 + GFF4357 0.41 -0.6 139,892 + GFF4357 0.54 -0.2 140,032 + GFF4357 0.62 -0.3 140,276 + GFF4357 0.75 -0.6 140,355 + GFF4357 0.79 -0.6 140,915 + -0.4 141,183 + GFF4358 0.75 -1.0 141,251 + -0.1 141,273 + -1.0 141,274 - -1.7 141,336 + +0.5 141,337 - -0.5 141,347 + -0.3 141,352 - -0.5 141,354 - -0.2 141,354 - -0.3 141,365 - +0.5 141,551 + GFF4359 0.43 +0.3 141,584 + GFF4359 0.51 -0.4 141,585 - GFF4359 0.52 +1.0 141,632 + GFF4359 0.63 -0.2
Or see this region's nucleotide sequence