Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4348 and GFF4349 are separated by 43 nucleotides GFF4349 and GFF4350 are separated by 7 nucleotides GFF4350 and GFF4351 are separated by 122 nucleotides GFF4351 and GFF4352 are separated by 346 nucleotides
GFF4348 - AmpG permease, at 128,812 to 130,287
GFF4348
GFF4349 - Hypothetical lipoprotein YajG precursor, at 130,331 to 130,963
GFF4349
GFF4350 - hypothetical protein, at 130,971 to 131,090
GFF4350
GFF4351 - Cell division protein BolA, at 131,213 to 131,530
GFF4351
GFF4352 - Cell division trigger factor (EC 5.2.1.8), at 131,877 to 133,175
GFF4352
Position (kb) 130
131
132 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 130.057 kb on + strand, within GFF4348 at 130.105 kb on - strand, within GFF4348 at 130.112 kb on + strand, within GFF4348 at 130.113 kb on - strand, within GFF4348 at 130.183 kb on + strand at 130.274 kb on + strand at 130.287 kb on + strand at 130.288 kb on - strand at 130.288 kb on - strand at 130.296 kb on - strand at 130.330 kb on - strand at 130.381 kb on + strand at 130.382 kb on - strand at 130.382 kb on - strand at 130.435 kb on + strand, within GFF4349 at 130.465 kb on - strand, within GFF4349 at 130.465 kb on - strand, within GFF4349 at 130.579 kb on - strand, within GFF4349 at 130.596 kb on - strand, within GFF4349 at 130.596 kb on - strand, within GFF4349 at 130.631 kb on - strand, within GFF4349 at 130.632 kb on + strand, within GFF4349 at 130.633 kb on - strand, within GFF4349 at 130.633 kb on - strand, within GFF4349 at 130.670 kb on + strand, within GFF4349 at 130.671 kb on - strand, within GFF4349 at 131.013 kb on - strand, within GFF4350 at 131.166 kb on + strand at 131.167 kb on - strand at 131.220 kb on + strand at 131.220 kb on + strand at 131.221 kb on - strand at 131.233 kb on - strand at 131.393 kb on + strand, within GFF4351 at 131.427 kb on + strand, within GFF4351 at 131.428 kb on - strand, within GFF4351 at 131.428 kb on - strand, within GFF4351 at 131.446 kb on + strand, within GFF4351 at 131.446 kb on + strand, within GFF4351 at 131.446 kb on + strand, within GFF4351 at 131.446 kb on + strand, within GFF4351 at 131.447 kb on - strand, within GFF4351 at 131.447 kb on - strand, within GFF4351 at 131.447 kb on - strand, within GFF4351 at 131.450 kb on + strand, within GFF4351 at 131.450 kb on + strand, within GFF4351 at 131.451 kb on - strand, within GFF4351 at 131.451 kb on - strand, within GFF4351 at 131.451 kb on - strand, within GFF4351 at 131.451 kb on - strand, within GFF4351 at 131.453 kb on - strand, within GFF4351 at 131.482 kb on + strand, within GFF4351 at 131.485 kb on + strand, within GFF4351 at 131.548 kb on + strand at 131.548 kb on + strand at 131.549 kb on - strand at 131.740 kb on + strand at 131.741 kb on - strand at 131.741 kb on - strand at 131.742 kb on + strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.820 kb on + strand at 131.996 kb on + strand at 131.996 kb on + strand at 131.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 130,057 + GFF4348 0.84 +0.0 130,105 - GFF4348 0.88 +0.3 130,112 + GFF4348 0.88 -1.7 130,113 - GFF4348 0.88 -0.3 130,183 + -2.1 130,274 + -0.4 130,287 + -1.0 130,288 - +0.2 130,288 - -0.6 130,296 - -0.7 130,330 - -0.0 130,381 + +0.1 130,382 - +0.2 130,382 - +0.3 130,435 + GFF4349 0.16 +0.1 130,465 - GFF4349 0.21 -0.1 130,465 - GFF4349 0.21 -0.0 130,579 - GFF4349 0.39 +0.1 130,596 - GFF4349 0.42 -2.1 130,596 - GFF4349 0.42 +0.3 130,631 - GFF4349 0.47 -1.6 130,632 + GFF4349 0.48 +0.2 130,633 - GFF4349 0.48 +0.3 130,633 - GFF4349 0.48 -0.5 130,670 + GFF4349 0.54 +0.5 130,671 - GFF4349 0.54 +0.1 131,013 - GFF4350 0.35 -1.0 131,166 + -0.3 131,167 - -0.9 131,220 + +0.1 131,220 + +1.1 131,221 - +0.2 131,233 - -0.2 131,393 + GFF4351 0.57 -0.2 131,427 + GFF4351 0.67 +0.7 131,428 - GFF4351 0.68 +0.1 131,428 - GFF4351 0.68 +0.0 131,446 + GFF4351 0.73 +0.4 131,446 + GFF4351 0.73 -0.0 131,446 + GFF4351 0.73 -0.0 131,446 + GFF4351 0.73 +0.8 131,447 - GFF4351 0.74 +1.0 131,447 - GFF4351 0.74 +0.5 131,447 - GFF4351 0.74 -0.7 131,450 + GFF4351 0.75 -0.8 131,450 + GFF4351 0.75 -0.4 131,451 - GFF4351 0.75 -0.7 131,451 - GFF4351 0.75 -1.1 131,451 - GFF4351 0.75 -0.2 131,451 - GFF4351 0.75 -2.0 131,453 - GFF4351 0.75 -3.2 131,482 + GFF4351 0.85 -0.9 131,485 + GFF4351 0.86 +1.3 131,548 + -0.7 131,548 + -0.7 131,549 - +0.1 131,740 + +0.8 131,741 - -1.1 131,741 - -0.1 131,742 + -2.9 131,743 - +0.1 131,743 - +0.2 131,820 + -0.8 131,996 + -0.7 131,996 + -0.6 131,997 - -1.9
Or see this region's nucleotide sequence