Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4049 and GFF4050 overlap by 4 nucleotides GFF4050 and GFF4051 are separated by 15 nucleotides GFF4051 and GFF4052 overlap by 4 nucleotides
GFF4049 - Uncharacterized protein ImpH/VasB, at 11,141 to 12,136
GFF4049
GFF4050 - Protein ImpG/VasA, at 12,133 to 14,016
GFF4050
GFF4051 - Uncharacterized protein ImpF, at 14,032 to 14,526
GFF4051
GFF4052 - Protein of avirulence locus ImpE, at 14,523 to 15,347
GFF4052
Position (kb) 12
13
14
15 Strain fitness (log2 ratio) -1
0
1
2 at 11.137 kb on - strand at 11.320 kb on + strand, within GFF4049 at 11.402 kb on - strand, within GFF4049 at 11.555 kb on + strand, within GFF4049 at 11.566 kb on - strand, within GFF4049 at 11.703 kb on + strand, within GFF4049 at 11.916 kb on + strand, within GFF4049 at 11.917 kb on - strand, within GFF4049 at 12.047 kb on - strand at 12.219 kb on + strand at 12.243 kb on + strand at 12.245 kb on + strand at 12.246 kb on - strand at 12.246 kb on - strand at 12.273 kb on - strand at 12.335 kb on + strand, within GFF4050 at 12.622 kb on + strand, within GFF4050 at 12.623 kb on - strand, within GFF4050 at 12.654 kb on + strand, within GFF4050 at 12.954 kb on - strand, within GFF4050 at 13.126 kb on + strand, within GFF4050 at 13.127 kb on - strand, within GFF4050 at 13.196 kb on + strand, within GFF4050 at 13.380 kb on + strand, within GFF4050 at 13.380 kb on + strand, within GFF4050 at 13.380 kb on + strand, within GFF4050 at 13.384 kb on + strand, within GFF4050 at 13.384 kb on + strand, within GFF4050 at 13.384 kb on + strand, within GFF4050 at 13.385 kb on - strand, within GFF4050 at 13.430 kb on + strand, within GFF4050 at 13.769 kb on + strand, within GFF4050 at 13.883 kb on + strand at 13.973 kb on + strand at 13.974 kb on - strand at 13.992 kb on - strand at 14.030 kb on + strand at 14.134 kb on + strand, within GFF4051 at 14.142 kb on + strand, within GFF4051 at 14.144 kb on + strand, within GFF4051 at 14.144 kb on + strand, within GFF4051 at 14.144 kb on + strand, within GFF4051 at 14.144 kb on + strand, within GFF4051 at 14.145 kb on - strand, within GFF4051 at 14.145 kb on - strand, within GFF4051 at 14.267 kb on + strand, within GFF4051 at 14.268 kb on - strand, within GFF4051 at 14.268 kb on - strand, within GFF4051 at 14.272 kb on - strand, within GFF4051 at 14.272 kb on - strand, within GFF4051 at 14.272 kb on - strand, within GFF4051 at 14.508 kb on + strand at 14.818 kb on - strand, within GFF4052 at 14.843 kb on - strand, within GFF4052
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 11,137 - +0.1 11,320 + GFF4049 0.18 -0.4 11,402 - GFF4049 0.26 -0.5 11,555 + GFF4049 0.42 -0.2 11,566 - GFF4049 0.43 +0.4 11,703 + GFF4049 0.56 -0.1 11,916 + GFF4049 0.78 +0.1 11,917 - GFF4049 0.78 +0.6 12,047 - -0.9 12,219 + -0.0 12,243 + +0.4 12,245 + +0.4 12,246 - +0.3 12,246 - +0.3 12,273 - +0.4 12,335 + GFF4050 0.11 -0.2 12,622 + GFF4050 0.26 +0.0 12,623 - GFF4050 0.26 +2.0 12,654 + GFF4050 0.28 +1.2 12,954 - GFF4050 0.44 +0.5 13,126 + GFF4050 0.53 +0.1 13,127 - GFF4050 0.53 +0.1 13,196 + GFF4050 0.56 -0.3 13,380 + GFF4050 0.66 +1.6 13,380 + GFF4050 0.66 -1.2 13,380 + GFF4050 0.66 -0.4 13,384 + GFF4050 0.66 -0.6 13,384 + GFF4050 0.66 +0.3 13,384 + GFF4050 0.66 +0.3 13,385 - GFF4050 0.66 +1.1 13,430 + GFF4050 0.69 +1.0 13,769 + GFF4050 0.87 -1.6 13,883 + -0.3 13,973 + +0.4 13,974 - +0.6 13,992 - -0.3 14,030 + -0.1 14,134 + GFF4051 0.21 -0.5 14,142 + GFF4051 0.22 +0.3 14,144 + GFF4051 0.23 +0.2 14,144 + GFF4051 0.23 +0.8 14,144 + GFF4051 0.23 +0.1 14,144 + GFF4051 0.23 +0.6 14,145 - GFF4051 0.23 -0.3 14,145 - GFF4051 0.23 +0.1 14,267 + GFF4051 0.47 +0.2 14,268 - GFF4051 0.48 -0.5 14,268 - GFF4051 0.48 +0.4 14,272 - GFF4051 0.48 +0.7 14,272 - GFF4051 0.48 -0.4 14,272 - GFF4051 0.48 +0.2 14,508 + +0.0 14,818 - GFF4052 0.36 -0.3 14,843 - GFF4052 0.39 +0.5
Or see this region's nucleotide sequence