Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3914 and GFF3915 are separated by 45 nucleotides GFF3915 and GFF3916 are separated by 285 nucleotides
GFF3914 - Nucleoside-diphosphate-sugar epimerases, at 195,598 to 196,422
GFF3914
GFF3915 - Hypothetical transcriptional regulator yeeY, at 196,468 to 197,382
GFF3915
GFF3916 - Putrescine importer, at 197,668 to 199,026
GFF3916
Position (kb) 196
197
198 Strain fitness (log2 ratio) -1
0
1 at 195.525 kb on + strand at 195.525 kb on + strand at 195.565 kb on + strand at 195.565 kb on + strand at 195.566 kb on - strand at 195.566 kb on - strand at 195.569 kb on + strand at 195.569 kb on + strand at 195.570 kb on - strand at 195.570 kb on - strand at 195.720 kb on + strand, within GFF3914 at 195.739 kb on + strand, within GFF3914 at 195.918 kb on + strand, within GFF3914 at 195.919 kb on - strand, within GFF3914 at 196.009 kb on - strand, within GFF3914 at 196.225 kb on + strand, within GFF3914 at 196.226 kb on - strand, within GFF3914 at 196.226 kb on - strand, within GFF3914 at 196.230 kb on + strand, within GFF3914 at 196.230 kb on + strand, within GFF3914 at 196.232 kb on + strand, within GFF3914 at 196.233 kb on - strand, within GFF3914 at 196.390 kb on + strand at 196.451 kb on - strand at 196.451 kb on - strand at 196.575 kb on + strand, within GFF3915 at 196.584 kb on + strand, within GFF3915 at 196.585 kb on - strand, within GFF3915 at 196.585 kb on - strand, within GFF3915 at 196.603 kb on - strand, within GFF3915 at 196.771 kb on - strand, within GFF3915 at 196.903 kb on - strand, within GFF3915 at 197.235 kb on - strand, within GFF3915 at 197.363 kb on - strand at 197.570 kb on + strand at 197.571 kb on - strand at 197.571 kb on - strand at 197.574 kb on + strand at 197.575 kb on - strand at 197.682 kb on + strand at 197.738 kb on + strand at 197.738 kb on + strand at 197.755 kb on + strand at 197.755 kb on + strand at 197.755 kb on + strand at 197.755 kb on + strand at 197.756 kb on - strand at 197.819 kb on + strand, within GFF3916 at 197.920 kb on + strand, within GFF3916 at 197.921 kb on - strand, within GFF3916 at 197.984 kb on - strand, within GFF3916 at 198.099 kb on + strand, within GFF3916 at 198.146 kb on + strand, within GFF3916
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 195,525 + +0.4 195,525 + +1.0 195,565 + -0.5 195,565 + +0.3 195,566 - +0.1 195,566 - -0.2 195,569 + +0.2 195,569 + +0.3 195,570 - +0.1 195,570 - +0.2 195,720 + GFF3914 0.15 +0.3 195,739 + GFF3914 0.17 -0.2 195,918 + GFF3914 0.39 +0.2 195,919 - GFF3914 0.39 +0.7 196,009 - GFF3914 0.50 +1.0 196,225 + GFF3914 0.76 -0.3 196,226 - GFF3914 0.76 -0.2 196,226 - GFF3914 0.76 -0.3 196,230 + GFF3914 0.77 -0.9 196,230 + GFF3914 0.77 -0.2 196,232 + GFF3914 0.77 -0.1 196,233 - GFF3914 0.77 +0.1 196,390 + +0.3 196,451 - -0.1 196,451 - +1.1 196,575 + GFF3915 0.12 -0.6 196,584 + GFF3915 0.13 +0.6 196,585 - GFF3915 0.13 +0.8 196,585 - GFF3915 0.13 +0.8 196,603 - GFF3915 0.15 +0.3 196,771 - GFF3915 0.33 -0.6 196,903 - GFF3915 0.48 -0.4 197,235 - GFF3915 0.84 -0.0 197,363 - +1.0 197,570 + +0.8 197,571 - -1.2 197,571 - +0.5 197,574 + -0.4 197,575 - -0.0 197,682 + -0.8 197,738 + +1.6 197,738 + -0.6 197,755 + -0.5 197,755 + +0.3 197,755 + +0.1 197,755 + +0.1 197,756 - +0.1 197,819 + GFF3916 0.11 +0.2 197,920 + GFF3916 0.19 +0.5 197,921 - GFF3916 0.19 +0.0 197,984 - GFF3916 0.23 -0.1 198,099 + GFF3916 0.32 -0.3 198,146 + GFF3916 0.35 +0.3
Or see this region's nucleotide sequence