Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF380 and GFF381 overlap by 8 nucleotides GFF381 and GFF382 are separated by 55 nucleotides GFF382 and GFF383 are separated by 69 nucleotides
GFF380 - UDP-glucose:(heptosyl) LPS alpha1,3-glucosyltransferase WaaG (EC 2.4.1.-), at 95,216 to 96,340
GFF380
GFF381 - Lipopolysaccharide core biosynthesis protein WaaP (EC 2.7.-.-), heptosyl-I-kinase, at 96,333 to 97,130
GFF381
GFF382 - UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,3-glucosyltransferase WaaO (EC 2.4.1.-), at 97,186 to 97,395
GFF382
GFF383 - Lipopolysaccharide 1,6-galactosyltransferase (EC 2.4.1.-), at 97,465 to 98,544
GFF383
Position (kb) 96
97
98 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 95.417 kb on + strand, within GFF380 at 95.576 kb on + strand, within GFF380 at 95.634 kb on + strand, within GFF380 at 95.635 kb on - strand, within GFF380 at 95.650 kb on + strand, within GFF380 at 95.651 kb on - strand, within GFF380 at 95.909 kb on + strand, within GFF380 at 95.910 kb on - strand, within GFF380 at 96.015 kb on + strand, within GFF380 at 96.036 kb on + strand, within GFF380 at 96.050 kb on + strand, within GFF380 at 96.137 kb on + strand, within GFF380 at 96.138 kb on - strand, within GFF380 at 96.139 kb on + strand, within GFF380 at 96.139 kb on + strand, within GFF380 at 96.139 kb on + strand, within GFF380 at 96.139 kb on + strand, within GFF380 at 96.266 kb on + strand at 96.285 kb on + strand at 96.319 kb on + strand at 96.319 kb on + strand at 96.320 kb on - strand at 96.330 kb on + strand at 96.364 kb on + strand at 96.488 kb on + strand, within GFF381 at 96.488 kb on + strand, within GFF381 at 96.533 kb on - strand, within GFF381 at 96.533 kb on - strand, within GFF381 at 96.536 kb on + strand, within GFF381 at 96.537 kb on - strand, within GFF381 at 96.602 kb on + strand, within GFF381 at 96.662 kb on + strand, within GFF381 at 96.663 kb on - strand, within GFF381 at 96.705 kb on + strand, within GFF381 at 96.725 kb on + strand, within GFF381 at 96.776 kb on + strand, within GFF381 at 96.776 kb on + strand, within GFF381
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 95,417 + GFF380 0.18 +3.5 95,576 + GFF380 0.32 +4.2 95,634 + GFF380 0.37 +4.5 95,635 - GFF380 0.37 +2.8 95,650 + GFF380 0.39 +3.7 95,651 - GFF380 0.39 +2.6 95,909 + GFF380 0.62 +4.7 95,910 - GFF380 0.62 +3.1 96,015 + GFF380 0.71 +5.6 96,036 + GFF380 0.73 +5.2 96,050 + GFF380 0.74 +5.5 96,137 + GFF380 0.82 +4.9 96,138 - GFF380 0.82 +2.4 96,139 + GFF380 0.82 +4.5 96,139 + GFF380 0.82 +4.6 96,139 + GFF380 0.82 +5.2 96,139 + GFF380 0.82 +4.9 96,266 + +5.5 96,285 + +0.2 96,319 + +0.3 96,319 + -0.2 96,320 - -2.7 96,330 + -2.0 96,364 + -3.1 96,488 + GFF381 0.19 -3.9 96,488 + GFF381 0.19 -2.6 96,533 - GFF381 0.25 -4.1 96,533 - GFF381 0.25 -3.5 96,536 + GFF381 0.25 -1.4 96,537 - GFF381 0.26 -1.3 96,602 + GFF381 0.34 -2.1 96,662 + GFF381 0.41 -3.1 96,663 - GFF381 0.41 -5.4 96,705 + GFF381 0.47 -2.1 96,725 + GFF381 0.49 -1.3 96,776 + GFF381 0.56 -1.3 96,776 + GFF381 0.56 -1.2
Or see this region's nucleotide sequence