Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3757 and GFF3758 overlap by 1 nucleotides GFF3758 and GFF3759 are separated by 9 nucleotides
GFF3757 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 24,698 to 25,723
GFF3757
GFF3758 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 25,723 to 26,817
GFF3758
GFF3759 - ABC transporter, periplasmic substrate-binding protein, at 26,827 to 28,632
GFF3759
Position (kb) 25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 24.967 kb on + strand, within GFF3757 at 24.968 kb on - strand, within GFF3757 at 25.024 kb on - strand, within GFF3757 at 25.408 kb on + strand, within GFF3757 at 25.415 kb on - strand, within GFF3757 at 25.424 kb on - strand, within GFF3757 at 25.598 kb on - strand, within GFF3757 at 25.650 kb on - strand at 25.724 kb on + strand at 25.814 kb on + strand at 26.147 kb on + strand, within GFF3758 at 26.165 kb on + strand, within GFF3758 at 26.223 kb on - strand, within GFF3758 at 26.225 kb on + strand, within GFF3758 at 26.293 kb on - strand, within GFF3758 at 26.406 kb on + strand, within GFF3758 at 26.490 kb on - strand, within GFF3758 at 26.490 kb on - strand, within GFF3758 at 26.808 kb on - strand at 26.826 kb on - strand at 26.826 kb on - strand at 26.828 kb on + strand at 26.871 kb on - strand at 26.895 kb on + strand at 26.896 kb on - strand at 26.896 kb on - strand at 26.896 kb on - strand at 26.896 kb on - strand at 26.900 kb on - strand at 26.959 kb on - strand at 26.959 kb on - strand at 26.959 kb on - strand at 26.960 kb on + strand at 26.960 kb on + strand at 26.960 kb on + strand at 26.961 kb on - strand at 26.979 kb on - strand at 26.981 kb on + strand at 27.106 kb on - strand, within GFF3759 at 27.235 kb on - strand, within GFF3759 at 27.259 kb on - strand, within GFF3759 at 27.259 kb on - strand, within GFF3759 at 27.360 kb on + strand, within GFF3759 at 27.471 kb on - strand, within GFF3759 at 27.578 kb on + strand, within GFF3759 at 27.605 kb on + strand, within GFF3759 at 27.618 kb on + strand, within GFF3759
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 24,967 + GFF3757 0.26 -1.3 24,968 - GFF3757 0.26 +0.0 25,024 - GFF3757 0.32 -0.9 25,408 + GFF3757 0.69 -0.3 25,415 - GFF3757 0.70 +0.0 25,424 - GFF3757 0.71 +0.3 25,598 - GFF3757 0.88 -0.2 25,650 - +0.1 25,724 + -0.7 25,814 + +1.8 26,147 + GFF3758 0.39 -3.3 26,165 + GFF3758 0.40 +0.1 26,223 - GFF3758 0.46 -0.8 26,225 + GFF3758 0.46 -0.1 26,293 - GFF3758 0.52 -0.9 26,406 + GFF3758 0.62 -1.4 26,490 - GFF3758 0.70 +1.3 26,490 - GFF3758 0.70 -1.4 26,808 - -0.3 26,826 - -0.5 26,826 - +0.7 26,828 + -2.0 26,871 - -0.4 26,895 + +0.0 26,896 - +0.2 26,896 - -0.2 26,896 - +0.2 26,896 - +0.3 26,900 - +0.9 26,959 - -0.3 26,959 - +0.4 26,959 - +0.1 26,960 + -0.6 26,960 + +0.2 26,960 + -0.0 26,961 - +0.1 26,979 - -0.2 26,981 + -0.2 27,106 - GFF3759 0.15 +0.4 27,235 - GFF3759 0.23 +0.5 27,259 - GFF3759 0.24 -0.1 27,259 - GFF3759 0.24 -0.5 27,360 + GFF3759 0.30 -0.1 27,471 - GFF3759 0.36 +1.7 27,578 + GFF3759 0.42 -0.7 27,605 + GFF3759 0.43 -0.0 27,618 + GFF3759 0.44 +0.5
Or see this region's nucleotide sequence