Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3430 and GFF3431 are separated by 377 nucleotides GFF3431 and GFF3432 are separated by 131 nucleotides GFF3432 and GFF3433 are separated by 44 nucleotides GFF3433 and GFF3434 are separated by 148 nucleotides GFF3434 and GFF3435 are separated by 338 nucleotides
GFF3430 - Periplasmic lysozyme inhibitor of c-type lysozyme, at 196,097 to 196,441
GFF3430
GFF3431 - hypothetical protein, at 196,819 to 196,944
GFF3431
GFF3432 - FIG01046599: hypothetical protein, at 197,076 to 197,249
GFF3432
GFF3433 - FIG074102: hypothetical protein, at 197,294 to 197,416
GFF3433
GFF3434 - Small heat shock protein, at 197,565 to 198,035
GFF3434
GFF3435 - FIG01046570: hypothetical protein, at 198,374 to 199,417
GFF3435
Position (kb) 197
198 Strain fitness (log2 ratio) -2
-1
0
1 at 196.305 kb on + strand, within GFF3430 at 196.346 kb on - strand, within GFF3430 at 196.351 kb on + strand, within GFF3430 at 196.351 kb on + strand, within GFF3430 at 196.442 kb on + strand at 196.493 kb on - strand at 196.521 kb on - strand at 196.586 kb on + strand at 196.732 kb on - strand at 196.835 kb on + strand, within GFF3431 at 196.835 kb on + strand, within GFF3431 at 196.974 kb on - strand at 197.331 kb on + strand, within GFF3433 at 197.502 kb on + strand at 197.502 kb on + strand at 197.502 kb on + strand at 197.724 kb on + strand, within GFF3434 at 197.724 kb on + strand, within GFF3434 at 197.724 kb on + strand, within GFF3434 at 197.798 kb on + strand, within GFF3434 at 198.122 kb on + strand at 198.142 kb on + strand at 198.142 kb on + strand at 198.146 kb on - strand at 198.244 kb on - strand at 198.264 kb on + strand at 198.305 kb on + strand at 198.356 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 196,305 + GFF3430 0.60 -1.4 196,346 - GFF3430 0.72 -0.5 196,351 + GFF3430 0.74 -1.7 196,351 + GFF3430 0.74 -0.5 196,442 + -0.1 196,493 - +1.4 196,521 - -2.5 196,586 + +0.1 196,732 - +0.1 196,835 + GFF3431 0.13 +0.1 196,835 + GFF3431 0.13 +0.2 196,974 - -0.7 197,331 + GFF3433 0.30 -0.7 197,502 + +0.1 197,502 + +0.3 197,502 + +0.1 197,724 + GFF3434 0.34 +0.2 197,724 + GFF3434 0.34 +0.1 197,724 + GFF3434 0.34 +0.4 197,798 + GFF3434 0.49 +0.1 198,122 + -0.1 198,142 + -0.2 198,142 + +0.8 198,146 - +0.3 198,244 - +1.0 198,264 + +0.1 198,305 + +0.6 198,356 + -0.0
Or see this region's nucleotide sequence