Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3205 and GFF3206 are separated by 207 nucleotides GFF3206 and GFF3207 are separated by 69 nucleotides
GFF3205 - Serine transporter, at 208,486 to 209,925
GFF3205
GFF3206 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 210,133 to 211,497
GFF3206
GFF3207 - 2-ketobutyrate formate-lyase (EC 2.3.1.-) / Pyruvate formate-lyase (EC 2.3.1.54), at 211,567 to 213,861
GFF3207
Position (kb) 210
211
212 Strain fitness (log2 ratio) -2
-1
0
1
2 at 209.136 kb on + strand, within GFF3205 at 209.136 kb on + strand, within GFF3205 at 209.151 kb on + strand, within GFF3205 at 209.200 kb on - strand, within GFF3205 at 209.200 kb on - strand, within GFF3205 at 209.202 kb on - strand, within GFF3205 at 209.202 kb on - strand, within GFF3205 at 209.245 kb on + strand, within GFF3205 at 209.388 kb on + strand, within GFF3205 at 209.388 kb on + strand, within GFF3205 at 209.397 kb on - strand, within GFF3205 at 209.477 kb on - strand, within GFF3205 at 209.557 kb on - strand, within GFF3205 at 209.580 kb on - strand, within GFF3205 at 209.599 kb on - strand, within GFF3205 at 209.601 kb on + strand, within GFF3205 at 209.601 kb on + strand, within GFF3205 at 209.601 kb on + strand, within GFF3205 at 209.602 kb on - strand, within GFF3205 at 209.602 kb on - strand, within GFF3205 at 209.692 kb on - strand, within GFF3205 at 209.692 kb on - strand, within GFF3205 at 209.716 kb on - strand, within GFF3205 at 209.792 kb on - strand at 209.803 kb on - strand at 209.838 kb on - strand at 210.094 kb on - strand at 210.106 kb on - strand at 210.313 kb on - strand, within GFF3206 at 210.321 kb on + strand, within GFF3206 at 210.321 kb on + strand, within GFF3206 at 210.335 kb on - strand, within GFF3206 at 210.399 kb on + strand, within GFF3206 at 210.400 kb on - strand, within GFF3206 at 210.521 kb on + strand, within GFF3206 at 210.521 kb on + strand, within GFF3206 at 210.522 kb on - strand, within GFF3206 at 210.565 kb on - strand, within GFF3206 at 210.600 kb on - strand, within GFF3206 at 210.705 kb on + strand, within GFF3206 at 211.068 kb on - strand, within GFF3206 at 211.070 kb on + strand, within GFF3206 at 211.113 kb on + strand, within GFF3206 at 211.114 kb on - strand, within GFF3206 at 211.161 kb on + strand, within GFF3206 at 211.278 kb on + strand, within GFF3206 at 211.497 kb on + strand at 211.497 kb on + strand at 211.497 kb on + strand at 211.497 kb on + strand at 211.568 kb on + strand at 211.569 kb on - strand at 211.582 kb on - strand at 211.597 kb on - strand at 211.750 kb on + strand at 211.751 kb on - strand at 211.751 kb on - strand at 211.751 kb on - strand at 212.066 kb on + strand, within GFF3207 at 212.150 kb on + strand, within GFF3207 at 212.336 kb on + strand, within GFF3207 at 212.351 kb on + strand, within GFF3207 at 212.352 kb on - strand, within GFF3207 at 212.378 kb on + strand, within GFF3207 at 212.378 kb on + strand, within GFF3207 at 212.432 kb on + strand, within GFF3207 at 212.432 kb on + strand, within GFF3207 at 212.433 kb on - strand, within GFF3207 at 212.433 kb on - strand, within GFF3207
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 209,136 + GFF3205 0.45 +1.3 209,136 + GFF3205 0.45 +0.6 209,151 + GFF3205 0.46 +0.1 209,200 - GFF3205 0.50 +0.4 209,200 - GFF3205 0.50 -0.1 209,202 - GFF3205 0.50 +0.6 209,202 - GFF3205 0.50 +0.5 209,245 + GFF3205 0.53 +0.2 209,388 + GFF3205 0.63 +1.3 209,388 + GFF3205 0.63 -0.8 209,397 - GFF3205 0.63 -0.1 209,477 - GFF3205 0.69 -0.4 209,557 - GFF3205 0.74 +0.4 209,580 - GFF3205 0.76 +0.1 209,599 - GFF3205 0.77 +0.4 209,601 + GFF3205 0.77 +1.2 209,601 + GFF3205 0.77 -0.1 209,601 + GFF3205 0.77 +0.4 209,602 - GFF3205 0.78 +0.6 209,602 - GFF3205 0.78 +0.1 209,692 - GFF3205 0.84 -1.2 209,692 - GFF3205 0.84 +0.2 209,716 - GFF3205 0.85 +0.2 209,792 - -1.2 209,803 - -1.8 209,838 - -0.0 210,094 - +0.4 210,106 - -0.4 210,313 - GFF3206 0.13 -0.1 210,321 + GFF3206 0.14 +0.1 210,321 + GFF3206 0.14 +0.2 210,335 - GFF3206 0.15 +0.1 210,399 + GFF3206 0.19 -0.4 210,400 - GFF3206 0.20 +0.0 210,521 + GFF3206 0.28 +0.1 210,521 + GFF3206 0.28 -0.1 210,522 - GFF3206 0.28 +0.2 210,565 - GFF3206 0.32 -0.0 210,600 - GFF3206 0.34 +0.9 210,705 + GFF3206 0.42 -1.0 211,068 - GFF3206 0.68 +0.0 211,070 + GFF3206 0.69 -0.2 211,113 + GFF3206 0.72 +0.6 211,114 - GFF3206 0.72 +0.3 211,161 + GFF3206 0.75 +0.7 211,278 + GFF3206 0.84 +0.6 211,497 + +0.8 211,497 + +0.5 211,497 + -0.0 211,497 + -0.1 211,568 + -0.8 211,569 - -0.2 211,582 - +2.2 211,597 - +0.1 211,750 + +0.5 211,751 - +0.6 211,751 - +0.2 211,751 - -1.1 212,066 + GFF3207 0.22 +0.1 212,150 + GFF3207 0.25 +0.6 212,336 + GFF3207 0.34 -0.8 212,351 + GFF3207 0.34 +0.0 212,352 - GFF3207 0.34 +0.2 212,378 + GFF3207 0.35 -0.2 212,378 + GFF3207 0.35 +1.2 212,432 + GFF3207 0.38 -0.1 212,432 + GFF3207 0.38 -1.4 212,433 - GFF3207 0.38 +0.3 212,433 - GFF3207 0.38 +0.1
Or see this region's nucleotide sequence