Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2996 and GFF2997 are separated by 115 nucleotides GFF2997 and GFF2998 are separated by 163 nucleotides GFF2998 and GFF2999 are separated by 158 nucleotides
GFF2996 - Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress, at 12,052 to 13,032
GFF2996
GFF2997 - COG1272: Predicted membrane protein hemolysin III homolog, at 13,148 to 13,807
GFF2997
GFF2998 - RNA-binding domain protein, at 13,971 to 14,282
GFF2998
GFF2999 - 6-phospho-beta-glucosidase (EC 3.2.1.86), at 14,441 to 15,874
GFF2999
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1 at 13.150 kb on - strand at 13.150 kb on - strand at 13.164 kb on + strand at 13.170 kb on + strand at 13.170 kb on + strand at 13.170 kb on + strand at 13.170 kb on + strand at 13.171 kb on - strand at 13.234 kb on - strand, within GFF2997 at 13.239 kb on - strand, within GFF2997 at 13.338 kb on + strand, within GFF2997 at 13.374 kb on + strand, within GFF2997 at 13.374 kb on + strand, within GFF2997 at 13.375 kb on - strand, within GFF2997 at 13.379 kb on - strand, within GFF2997 at 13.464 kb on - strand, within GFF2997 at 13.512 kb on + strand, within GFF2997 at 13.512 kb on + strand, within GFF2997 at 13.513 kb on - strand, within GFF2997 at 13.513 kb on - strand, within GFF2997 at 13.513 kb on - strand, within GFF2997 at 13.724 kb on + strand, within GFF2997 at 13.744 kb on + strand at 13.747 kb on + strand at 13.807 kb on + strand at 13.807 kb on + strand at 13.808 kb on - strand at 13.812 kb on + strand at 13.813 kb on - strand at 13.895 kb on - strand at 14.057 kb on + strand, within GFF2998 at 14.166 kb on + strand, within GFF2998 at 14.167 kb on - strand, within GFF2998 at 14.211 kb on - strand, within GFF2998 at 14.322 kb on + strand at 14.382 kb on - strand at 14.382 kb on - strand at 14.422 kb on - strand at 14.518 kb on + strand at 14.612 kb on + strand, within GFF2999 at 14.860 kb on - strand, within GFF2999 at 14.944 kb on + strand, within GFF2999 at 14.945 kb on - strand, within GFF2999 at 14.945 kb on - strand, within GFF2999 at 14.955 kb on - strand, within GFF2999 at 15.035 kb on + strand, within GFF2999 at 15.068 kb on + strand, within GFF2999 at 15.069 kb on - strand, within GFF2999 at 15.078 kb on + strand, within GFF2999 at 15.078 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.177 kb on - strand, within GFF2999 at 15.190 kb on + strand, within GFF2999 at 15.245 kb on - strand, within GFF2999 at 15.278 kb on + strand, within GFF2999
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 13,150 - -0.1 13,150 - +0.1 13,164 + +0.4 13,170 + +0.5 13,170 + -0.1 13,170 + +0.5 13,170 + -0.4 13,171 - -0.1 13,234 - GFF2997 0.13 -0.1 13,239 - GFF2997 0.14 -0.1 13,338 + GFF2997 0.29 +0.2 13,374 + GFF2997 0.34 -0.6 13,374 + GFF2997 0.34 +0.4 13,375 - GFF2997 0.34 +0.6 13,379 - GFF2997 0.35 -0.3 13,464 - GFF2997 0.48 +0.0 13,512 + GFF2997 0.55 -0.5 13,512 + GFF2997 0.55 -0.2 13,513 - GFF2997 0.55 -1.0 13,513 - GFF2997 0.55 +0.2 13,513 - GFF2997 0.55 -0.2 13,724 + GFF2997 0.87 +0.2 13,744 + +0.2 13,747 + -0.8 13,807 + +1.4 13,807 + -0.4 13,808 - +0.8 13,812 + -0.2 13,813 - -0.6 13,895 - +0.9 14,057 + GFF2998 0.28 -1.9 14,166 + GFF2998 0.62 -0.4 14,167 - GFF2998 0.63 -1.4 14,211 - GFF2998 0.77 -1.8 14,322 + -0.4 14,382 - -0.5 14,382 - +0.3 14,422 - +0.1 14,518 + -1.2 14,612 + GFF2999 0.12 -0.1 14,860 - GFF2999 0.29 -1.8 14,944 + GFF2999 0.35 +0.2 14,945 - GFF2999 0.35 -1.4 14,945 - GFF2999 0.35 +0.4 14,955 - GFF2999 0.36 -0.7 15,035 + GFF2999 0.41 -0.8 15,068 + GFF2999 0.44 -0.1 15,069 - GFF2999 0.44 +1.2 15,078 + GFF2999 0.44 -0.2 15,078 + GFF2999 0.44 -0.2 15,154 + GFF2999 0.50 -0.0 15,154 + GFF2999 0.50 +0.3 15,154 + GFF2999 0.50 +0.6 15,154 + GFF2999 0.50 -0.5 15,177 - GFF2999 0.51 -0.5 15,190 + GFF2999 0.52 +0.1 15,245 - GFF2999 0.56 -0.7 15,278 + GFF2999 0.58 -1.4
Or see this region's nucleotide sequence