Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2961

Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2960 and GFF2961 are separated by 82 nucleotidesGFF2961 and GFF2962 overlap by 4 nucleotidesGFF2962 and GFF2963 overlap by 4 nucleotides GFF2960 - Cytosine deaminase (EC 3.5.4.1), at 44,554 to 45,834 GFF2960 GFF2961 - Putative sugar isomerase involved in processing of exogenous sialic acid, at 45,917 to 46,384 GFF2961 GFF2962 - N-acetylmannosamine kinase (EC 2.7.1.60), at 46,381 to 47,256 GFF2962 GFF2963 - N-acetylmannosamine-6-phosphate 2-epimerase (EC 5.1.3.9), at 47,253 to 47,942 GFF2963 Position (kb) 45 46 47Strain fitness (log2 ratio) -2 -1 0 1 2at 45.128 kb on - strand, within GFF2960at 45.173 kb on - strand, within GFF2960at 45.300 kb on + strand, within GFF2960at 45.549 kb on + strand, within GFF2960at 45.762 kb on - strandat 45.762 kb on - strandat 46.049 kb on - strand, within GFF2961at 46.163 kb on + strand, within GFF2961at 46.166 kb on + strand, within GFF2961at 46.182 kb on + strand, within GFF2961at 46.239 kb on - strand, within GFF2961at 46.368 kb on - strandat 46.384 kb on + strandat 46.384 kb on + strandat 46.384 kb on + strandat 46.384 kb on + strandat 46.385 kb on - strandat 46.385 kb on - strandat 46.421 kb on - strandat 46.467 kb on - strandat 46.762 kb on + strand, within GFF2962at 46.882 kb on + strand, within GFF2962at 46.916 kb on + strand, within GFF2962at 47.074 kb on + strand, within GFF2962at 47.224 kb on - strandat 47.250 kb on + strandat 47.251 kb on - strandat 47.251 kb on - strandat 47.382 kb on - strand, within GFF2963at 47.382 kb on - strand, within GFF2963at 47.383 kb on + strand, within GFF2963

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI
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45,128 - GFF2960 0.45 -0.1
45,173 - GFF2960 0.48 +0.1
45,300 + GFF2960 0.58 +2.7
45,549 + GFF2960 0.78 +0.3
45,762 - -0.1
45,762 - +1.3
46,049 - GFF2961 0.28 -2.5
46,163 + GFF2961 0.53 +0.4
46,166 + GFF2961 0.53 +0.5
46,182 + GFF2961 0.57 +0.5
46,239 - GFF2961 0.69 +0.0
46,368 - -1.1
46,384 + -0.2
46,384 + +0.4
46,384 + -0.7
46,384 + -1.1
46,385 - -2.2
46,385 - +2.6
46,421 - +0.1
46,467 - -0.4
46,762 + GFF2962 0.43 -0.0
46,882 + GFF2962 0.57 +0.4
46,916 + GFF2962 0.61 +0.3
47,074 + GFF2962 0.79 +0.4
47,224 - +1.9
47,250 + -0.2
47,251 - +0.5
47,251 - +0.2
47,382 - GFF2963 0.19 +1.6
47,382 - GFF2963 0.19 +0.1
47,383 + GFF2963 0.19 +0.1

Or see this region's nucleotide sequence