Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2180 and GFF2181 are separated by 16 nucleotides GFF2181 and GFF2182 are separated by 174 nucleotides
GFF2180 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 26,463 to 27,341
GFF2180
GFF2181 - Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor, at 27,358 to 28,392
GFF2181
GFF2182 - Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), at 28,567 to 29,280
GFF2182
Position (kb) 27
28
29 Strain fitness (log2 ratio) -1
0
1 at 27.364 kb on + strand at 27.365 kb on - strand at 27.550 kb on + strand, within GFF2181 at 27.550 kb on + strand, within GFF2181 at 27.675 kb on - strand, within GFF2181 at 27.948 kb on + strand, within GFF2181 at 28.074 kb on - strand, within GFF2181 at 28.077 kb on + strand, within GFF2181 at 28.078 kb on - strand, within GFF2181 at 28.195 kb on + strand, within GFF2181 at 28.264 kb on + strand, within GFF2181 at 28.265 kb on - strand, within GFF2181 at 28.286 kb on + strand, within GFF2181 at 28.329 kb on + strand at 28.329 kb on + strand at 28.390 kb on + strand at 28.391 kb on - strand at 28.906 kb on - strand, within GFF2182 at 28.965 kb on + strand, within GFF2182 at 29.049 kb on - strand, within GFF2182 at 29.116 kb on - strand, within GFF2182 at 29.240 kb on - strand at 29.278 kb on + strand at 29.344 kb on + strand at 29.344 kb on + strand at 29.385 kb on + strand at 29.386 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 27,364 + +0.2 27,365 - +1.4 27,550 + GFF2181 0.19 -1.0 27,550 + GFF2181 0.19 -0.5 27,675 - GFF2181 0.31 -0.1 27,948 + GFF2181 0.57 -0.7 28,074 - GFF2181 0.69 +1.7 28,077 + GFF2181 0.69 -0.1 28,078 - GFF2181 0.70 -0.6 28,195 + GFF2181 0.81 -0.6 28,264 + GFF2181 0.88 +1.0 28,265 - GFF2181 0.88 -0.5 28,286 + GFF2181 0.90 -0.7 28,329 + -0.3 28,329 + +0.5 28,390 + +0.1 28,391 - -0.5 28,906 - GFF2182 0.47 -0.2 28,965 + GFF2182 0.56 +0.7 29,049 - GFF2182 0.68 +0.3 29,116 - GFF2182 0.77 -0.3 29,240 - +1.0 29,278 + -0.2 29,344 + -1.5 29,344 + +0.7 29,385 + -0.3 29,386 - -0.3
Or see this region's nucleotide sequence