Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2165 and GFF2166 are separated by 35 nucleotides GFF2166 and GFF2167 are separated by 4 nucleotides
GFF2165 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 10,712 to 12,799
GFF2165
GFF2166 - Exopolyphosphatase (EC 3.6.1.11), at 12,835 to 14,376
GFF2166
GFF2167 - Polyphosphate kinase (EC 2.7.4.1), at 14,381 to 16,447
GFF2167
Position (kb) 12
13
14
15 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 11.858 kb on - strand, within GFF2165 at 11.858 kb on - strand, within GFF2165 at 11.860 kb on + strand, within GFF2165 at 11.861 kb on - strand, within GFF2165 at 11.918 kb on + strand, within GFF2165 at 12.131 kb on - strand, within GFF2165 at 12.155 kb on + strand, within GFF2165 at 12.155 kb on + strand, within GFF2165 at 12.310 kb on + strand, within GFF2165 at 12.311 kb on - strand, within GFF2165 at 12.335 kb on - strand, within GFF2165 at 12.539 kb on + strand, within GFF2165 at 12.649 kb on + strand at 12.676 kb on + strand at 12.762 kb on + strand at 12.890 kb on - strand at 13.145 kb on + strand, within GFF2166 at 13.215 kb on + strand, within GFF2166 at 13.216 kb on - strand, within GFF2166 at 13.234 kb on + strand, within GFF2166 at 13.401 kb on - strand, within GFF2166 at 13.401 kb on - strand, within GFF2166 at 13.475 kb on - strand, within GFF2166 at 13.717 kb on - strand, within GFF2166 at 13.759 kb on - strand, within GFF2166 at 13.850 kb on + strand, within GFF2166 at 13.984 kb on - strand, within GFF2166 at 14.101 kb on + strand, within GFF2166 at 14.106 kb on + strand, within GFF2166 at 14.116 kb on + strand, within GFF2166 at 14.122 kb on - strand, within GFF2166 at 14.150 kb on - strand, within GFF2166 at 14.178 kb on + strand, within GFF2166 at 14.178 kb on + strand, within GFF2166 at 14.215 kb on - strand, within GFF2166 at 14.254 kb on + strand at 14.304 kb on + strand at 14.304 kb on + strand at 14.410 kb on - strand at 14.410 kb on - strand at 14.440 kb on + strand at 14.638 kb on + strand, within GFF2167 at 14.659 kb on + strand, within GFF2167 at 14.659 kb on + strand, within GFF2167 at 14.904 kb on + strand, within GFF2167
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 11,858 - GFF2165 0.55 -0.3 11,858 - GFF2165 0.55 +0.2 11,860 + GFF2165 0.55 +0.5 11,861 - GFF2165 0.55 -0.4 11,918 + GFF2165 0.58 -0.4 12,131 - GFF2165 0.68 +0.5 12,155 + GFF2165 0.69 -1.5 12,155 + GFF2165 0.69 +0.8 12,310 + GFF2165 0.77 +0.7 12,311 - GFF2165 0.77 +0.9 12,335 - GFF2165 0.78 -0.6 12,539 + GFF2165 0.88 -1.5 12,649 + -0.2 12,676 + +0.1 12,762 + +0.4 12,890 - -0.3 13,145 + GFF2166 0.20 -0.9 13,215 + GFF2166 0.25 -0.5 13,216 - GFF2166 0.25 -0.9 13,234 + GFF2166 0.26 -1.5 13,401 - GFF2166 0.37 +0.4 13,401 - GFF2166 0.37 -0.9 13,475 - GFF2166 0.42 -2.7 13,717 - GFF2166 0.57 +0.1 13,759 - GFF2166 0.60 -0.9 13,850 + GFF2166 0.66 -2.9 13,984 - GFF2166 0.75 -0.6 14,101 + GFF2166 0.82 -1.5 14,106 + GFF2166 0.82 -2.1 14,116 + GFF2166 0.83 -2.8 14,122 - GFF2166 0.83 +0.0 14,150 - GFF2166 0.85 +0.4 14,178 + GFF2166 0.87 +0.2 14,178 + GFF2166 0.87 -1.1 14,215 - GFF2166 0.89 -1.2 14,254 + -1.3 14,304 + -0.6 14,304 + -0.1 14,410 - -0.1 14,410 - +1.1 14,440 + -1.5 14,638 + GFF2167 0.12 -0.7 14,659 + GFF2167 0.13 -0.3 14,659 + GFF2167 0.13 +1.0 14,904 + GFF2167 0.25 +1.1
Or see this region's nucleotide sequence