Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF209 and GFF210 are separated by 53 nucleotides GFF210 and GFF211 are separated by 108 nucleotides GFF211 and GFF212 are separated by 76 nucleotides
GFF209 - Crotonobetaine carnitine-CoA ligase (EC 6.3.2.-), at 204,973 to 206,526
GFF209
GFF210 - Carnitine racemase (EC 5.-.-.-) / Carnitinyl-CoA dehydratase (EC 4.2.1.-), at 206,580 to 207,365
GFF210
GFF211 - Carnitine operon protein CaiE, at 207,474 to 208,070
GFF211
GFF212 - Transcriptional activatory protein CaiF, at 208,147 to 208,542
GFF212
Position (kb) 206
207
208 Strain fitness (log2 ratio) -2
-1
0
1
2 at 205.583 kb on - strand, within GFF209 at 205.709 kb on + strand, within GFF209 at 205.750 kb on - strand, within GFF209 at 206.013 kb on + strand, within GFF209 at 206.014 kb on - strand, within GFF209 at 206.017 kb on + strand, within GFF209 at 206.018 kb on - strand, within GFF209 at 206.018 kb on - strand, within GFF209 at 206.086 kb on - strand, within GFF209 at 206.106 kb on + strand, within GFF209 at 206.107 kb on - strand, within GFF209 at 206.122 kb on + strand, within GFF209 at 206.122 kb on + strand, within GFF209 at 206.122 kb on + strand, within GFF209 at 206.123 kb on - strand, within GFF209 at 206.171 kb on + strand, within GFF209 at 206.332 kb on - strand, within GFF209 at 206.379 kb on + strand at 206.380 kb on - strand at 206.560 kb on + strand at 206.627 kb on + strand at 206.776 kb on + strand, within GFF210 at 207.211 kb on - strand, within GFF210 at 207.402 kb on + strand at 207.467 kb on + strand at 207.468 kb on - strand at 207.473 kb on + strand at 207.473 kb on + strand at 207.473 kb on + strand at 207.474 kb on - strand at 207.474 kb on - strand at 207.474 kb on - strand at 207.481 kb on - strand at 207.481 kb on - strand at 207.579 kb on + strand, within GFF211 at 207.580 kb on - strand, within GFF211 at 207.581 kb on + strand, within GFF211 at 207.641 kb on + strand, within GFF211 at 207.663 kb on - strand, within GFF211 at 207.663 kb on - strand, within GFF211 at 207.672 kb on + strand, within GFF211 at 207.731 kb on - strand, within GFF211 at 207.782 kb on + strand, within GFF211 at 207.896 kb on - strand, within GFF211 at 207.948 kb on + strand, within GFF211 at 208.069 kb on - strand at 208.069 kb on - strand at 208.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 205,583 - GFF209 0.39 +0.5 205,709 + GFF209 0.47 +0.6 205,750 - GFF209 0.50 +0.3 206,013 + GFF209 0.67 -0.2 206,014 - GFF209 0.67 -1.4 206,017 + GFF209 0.67 +0.1 206,018 - GFF209 0.67 -0.9 206,018 - GFF209 0.67 +1.1 206,086 - GFF209 0.72 +0.3 206,106 + GFF209 0.73 -1.1 206,107 - GFF209 0.73 +0.2 206,122 + GFF209 0.74 +0.7 206,122 + GFF209 0.74 +0.7 206,122 + GFF209 0.74 -0.2 206,123 - GFF209 0.74 +0.3 206,171 + GFF209 0.77 +0.1 206,332 - GFF209 0.87 +0.6 206,379 + +0.5 206,380 - -0.9 206,560 + +0.1 206,627 + +0.6 206,776 + GFF210 0.25 +0.1 207,211 - GFF210 0.80 +0.8 207,402 + +0.0 207,467 + +0.7 207,468 - +0.0 207,473 + -0.3 207,473 + -0.4 207,473 + +0.1 207,474 - +2.7 207,474 - +0.1 207,474 - +0.0 207,481 - +0.0 207,481 - -0.2 207,579 + GFF211 0.18 -2.5 207,580 - GFF211 0.18 +0.2 207,581 + GFF211 0.18 +0.2 207,641 + GFF211 0.28 +0.4 207,663 - GFF211 0.32 +0.5 207,663 - GFF211 0.32 -0.2 207,672 + GFF211 0.33 +0.4 207,731 - GFF211 0.43 +0.3 207,782 + GFF211 0.52 -0.1 207,896 - GFF211 0.71 -0.2 207,948 + GFF211 0.79 -0.1 208,069 - +0.6 208,069 - -0.5 208,114 + -0.5
Or see this region's nucleotide sequence