Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2073 and GFF2074 are separated by 11 nucleotides GFF2074 and GFF2075 overlap by 4 nucleotides
GFF2073 - Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7), at 35,270 to 36,631
GFF2073
GFF2074 - Ethanolamine utilization protein EutA, at 36,643 to 38,046
GFF2074
GFF2075 - Ethanolamine permease, at 38,043 to 39,269
GFF2075
Position (kb) 36
37
38
39 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 35.701 kb on + strand, within GFF2073 at 35.701 kb on + strand, within GFF2073 at 35.702 kb on - strand, within GFF2073 at 35.778 kb on + strand, within GFF2073 at 35.779 kb on - strand, within GFF2073 at 36.115 kb on + strand, within GFF2073 at 36.122 kb on + strand, within GFF2073 at 36.336 kb on + strand, within GFF2073 at 36.590 kb on - strand at 36.719 kb on + strand at 36.720 kb on - strand at 36.720 kb on - strand at 36.720 kb on - strand at 36.773 kb on - strand at 36.787 kb on + strand, within GFF2074 at 36.788 kb on - strand, within GFF2074 at 36.788 kb on - strand, within GFF2074 at 37.069 kb on - strand, within GFF2074 at 37.072 kb on - strand, within GFF2074 at 37.092 kb on + strand, within GFF2074 at 37.242 kb on - strand, within GFF2074 at 37.285 kb on - strand, within GFF2074 at 37.285 kb on - strand, within GFF2074 at 37.569 kb on - strand, within GFF2074 at 37.777 kb on - strand, within GFF2074 at 37.908 kb on + strand at 37.909 kb on - strand at 38.193 kb on + strand, within GFF2075 at 38.291 kb on + strand, within GFF2075 at 38.292 kb on - strand, within GFF2075 at 38.391 kb on + strand, within GFF2075 at 38.490 kb on - strand, within GFF2075 at 38.893 kb on - strand, within GFF2075 at 38.905 kb on + strand, within GFF2075 at 38.905 kb on + strand, within GFF2075 at 39.022 kb on - strand, within GFF2075
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 35,701 + GFF2073 0.32 -0.5 35,701 + GFF2073 0.32 +0.3 35,702 - GFF2073 0.32 +0.6 35,778 + GFF2073 0.37 +0.5 35,779 - GFF2073 0.37 -0.0 36,115 + GFF2073 0.62 -0.2 36,122 + GFF2073 0.63 +1.3 36,336 + GFF2073 0.78 +0.0 36,590 - +1.2 36,719 + +0.2 36,720 - -3.5 36,720 - +0.4 36,720 - +0.3 36,773 - +0.8 36,787 + GFF2074 0.10 -0.8 36,788 - GFF2074 0.10 -1.2 36,788 - GFF2074 0.10 -0.1 37,069 - GFF2074 0.30 +0.1 37,072 - GFF2074 0.31 +0.2 37,092 + GFF2074 0.32 +0.2 37,242 - GFF2074 0.43 +0.3 37,285 - GFF2074 0.46 +1.2 37,285 - GFF2074 0.46 +0.9 37,569 - GFF2074 0.66 +0.5 37,777 - GFF2074 0.81 +0.1 37,908 + -0.3 37,909 - +1.1 38,193 + GFF2075 0.12 -0.7 38,291 + GFF2075 0.20 +0.4 38,292 - GFF2075 0.20 +0.1 38,391 + GFF2075 0.28 +0.2 38,490 - GFF2075 0.36 +0.8 38,893 - GFF2075 0.69 +1.8 38,905 + GFF2075 0.70 +1.1 38,905 + GFF2075 0.70 +1.8 39,022 - GFF2075 0.80 +0.9
Or see this region's nucleotide sequence